| Project/Area Number |
03454441
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Okayama University Dental School |
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Principal Investigator |
MURAYAMA Yoji Okayama Univ. Dental Sch., Professor, 歯学部, 教授 (50029972)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Hideki Okayama Univ. Dental Sch., Assistant Professor, 歯学部附属病院, 講師 (70170983)
TAKASHIBA Shogo Okayama Univ. Dental Sch., Assistant, 歯学部附属病院, 助手 (50226768)
TAKAHASHI Keiso Okayama Univ. Dental Sch., Assistant, 歯学部附属病院, 助手 (70243475)
ISOSHIMA Osamu Okayama Univ. Dental Sch., Assistant Professor, 歯学部附属病院, 講師 (90176256)
KURIHARA Hidemi Okayama Univ. Dental Sch., Associate Professor, 歯学部, 助教授 (40161765)
滝川 雅之 岡山大学, 歯学部・附属病院, 助手 (60243474)
永井 淳 岡山大学, 歯学部・附属病院, 講師 (70252989)
阿久津 功 岡山大学, 歯学部附属病院, 助手 (80231851)
野村 慶雄 岡山大学, 歯学部, 助教授 (50107075)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1991: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | early-onset periodontitis / CD11 / CD18 / aggregation / mRNA |
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| Research Abstract |
We analyzed the cell-cell adherence related to CD11/CD18 and CD18 mRNA of the individuals with decreased CD11/CD18 expression on the surface of their neutrophils. Epstein Barr virus-transformed B cell lines were developed from one localized juvenile periodontitis (LIP) patient characterized by decreased CD11/CD18 on the neutrophils in peripheral blood and without any remarkable systemic diseases, two siblings with generalized prepubertal periodontitis (GPP) caused from leukocyte adhesion deficiency (LAD), another LIP patient, one localized prepubertal periodontitis (LPP) patient, and two healthy subjects. Adhesion of leukocytes to each other was measured as cluster formation by aggregation assay. The length and the amount of CD18 mRNA expressed in the cell lines were analyzed by Northern blotting using the 32p-labeled CD18 cDNA.The coding region of the mRNA was analyzed by the reverse transcription-polymerase chain reaction method. Base-mismatches between CD18 mRNA and the 32p-labeled RNA probe synthesized from Cd18 cDNA were analyzed by RNase protection assay. In the adherence assay, cells from the LIP patients with decreased CD11/CD18 formed more clusters of smaller size and with fewer cells than those of did the patients and healthy subjects, while the cells from both GPP patients were found not to aggregate and not to form clusters either in the absence or presence of PMA.There were no differences in the length and the amount of mRNA between the LIP patient and the other patients and healthy subjects, while GPP patients expressed a small amount of long mRNA.The whole coding region (2,313 base pairs) was amplified from all of subjects except the GPP Patients, while the 5'-region (1,119 base pairs) was amplified from all subjects. Base-mismatches were detected on the coding region from 965 to 1,450 nucleotides of CD18 mRNA in GPPpatients. No mismatch was detected on other regions of CD18 mRNA in any subject. These results suggest that the LIP patient with an anom
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