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The study of using cultured fibroblast cells to the preiodontal tissue regeneration.

Research Project

Project/Area Number 03454444
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionSHOWA UNIVERSITY

Principal Investigator

MIYASHIMA Hajime  Showa Univ. Periodontics, Professor, 歯学部, 教授 (60014122)

Co-Investigator(Kenkyū-buntansha) TACHIKAWA Testuhiko  Showa Univ. Oral Pathology, Assistant Professor, 歯学部, 助教授 (10085772)
TANAKA Hiroko  Showa Univ. Periodontics, Assistant, 歯学部, 助手 (30217070)
犬井 清孝  昭和大学, 歯学部, 助手 (00213132)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsbone regeneration / cell cultured / cell maker / cultured cell implant / alteration of periodontal ligament cell / 再生 / 組織学的観察 / GTR法
Research Abstract

It's necessary to keep more over absolute number of cells for the regeneration of periodontally destructed defects.
Especialy, fibroblast like cells sourced from periodontal ligaments have important part for the regeneration of alveolar bone, cementum and periodontal ligament.
In this study, it had taked the periodontal ligament cells from extraeted teeth surface of the dog.
The fibloblast like cells were dyed by fluorescent marker kit (Zynaxis, USA) after 4-6 successive cultured in vitro. The fibroblast like cells were well dyed.
The cells that dyed had been cultured futher ore, it was assumed that devided nucleus of these cells were almost the same dyed as originals.
These cells were in placed the defects of the furcation area of the same dog.
Flap procedure were made from vestiblular side to keep epithelial attachment.
The bone of implanted collagen gel by itself was higher than control.
Fibroblast like cells mixed into collagen gel was higher than collagen gel by itself.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2018-02-02  

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