| Project/Area Number |
03454457
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
外科・放射線系歯学
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| Research Institution | TOKYO MEDICAL AND DENTAL UNIVERSITY |
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Principal Investigator |
SUZUKI Nagaaki Tokyo Medical and Dental University, School of Dentistry, Associate Professor, 歯学部, 助教授 (80014108)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1991: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | pain / central nervous system / cerebral cortex / deoxyglucose / デオキシグルコ-ス法 |
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| Research Abstract |
The effect of electrical stimulation of the trigeminal nerve on the C-14 deoxyglucose concentration in the somatosensory cortex was studied in thiamylal anesthetized rats. Experiments were carried out on 12 Wistar albino rats weighing 500 to 550 g. Each rat was anesthetied with thiamylal sodium given intraperitoneally at a dose of 200 mg/kg. C-14 deoxyglucose was injected intravenously at a dose of 50 muCi. The rats were divided into two groups. A bipolar stimulating electrode (interpolar distance : 2mm) of stainless steel wire was inserted into the right upper lip of 6 rats. The upper lip was stimulated electrically by rectangular pulses with a duration of 0.1 ms at 1Hz for 45 minutes. The remaining 6 rats were not electrically stimulated. Animals were killed with an overdose of thiamylal sodium. The brains were removed and frozen in isopentane (-50ﾟC). Coronal sections, 20 mum thick, of brain were taken serially and exposed, along with a set of previously calibrated autoradiographic C-14 micro-scale, on X-ray film for 3 weeks. Optical density was measured with a microdensitometer. In the stimulated group, an increase in optical density was evident in the contralateral (left) somatosentsory cortex. C-14 deoxyglucose concentrations in the contralateral somatosensory cortex were 9-29% higher than those in the ipsilateral somatosensory cortex. In the unstimulated group, there were no differences in C-14 deoxyglucose concentrations in the somatosensory cortex between the left and right sides. The present results indicate that electrical stimulation of the trigeminal nerve produced an increase in glucose utilization and neuronal activity in the contralatelal somatosensory cortex.
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