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An analysis on oncogene mutations in oral leukoplakia with PCR-SSCP

Research Project

Project/Area Number 03454465
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionOsaka University

Principal Investigator

ISHIDA Takeshi  Osaka Univ.Dent.Sch.Hosp., Clicical Laboratory, Assoc.Prof., 歯学部・附属病院, 助教授 (50028768)

Co-Investigator(Kenkyū-buntansha) KISHINO Mitsunobu  Osaka Univ.Dent.Sch.Hosp., Clicical Laboratory, Resident., 歯学部・附属病院, 医員
FUKUDA Yasuo  Osaka Univ.Dent.Sch.Hosp., Clicical Laboratory, Research Assist., 歯学部・附属病院, 助手 (20238489)
ISHIDA Takeshi  Osaka Univ.Dent.Sch.Hosp., Clicical Laboratory, Assoc.Prof. (50028768)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsTongue / Squamous cell carcinoma / Leukoplakia / p53 / Gene mutations / DNA増幅 / PCR
Research Abstract

The role of p53 tumor-suppressor gene in the carcinogenesis of oral squamous cell carcinoma is still unclear. The purpose of this research was to investigate the frequencies of p53 gene mutations in oral squamous cell carcinoma and leukoplakia by means of PCR-SSCP.
The template DNA was extracted from formalin-fixed, paraffin-embedded specimens (11 cases of tongue squamous cell carcinoma, 9 of tongue leukoplakia and 2 normal gingiva) by Proteinase K digestion method. PCR was performed on each sample with 4 primer with pairs for exon 5,6,7 and 8 of p53 gene. PCR products were electrophoresed in native gradient polyacrylamide gel and detected by silver staining (PCR-SSCP). Extra bands indicating the presence of mutations were detected in 1 case of squamous cell carcinoma and 1 case of leukoplakia.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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