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Biological and clinical study on regulation of gene expression in osteoblast and its osteogenesis

Research Project

Project/Area Number 03454468
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionThe University of Tokushima

Principal Investigator

NAGAYAMA Masaru  Tokushima University School of Dentistry Professor, 歯学部, 教授 (30022867)

Co-Investigator(Kenkyū-buntansha) NAKANISHI Hiroaki  Tokushima University School of Dontistry Research Associate, 歯学部・附属病院, 助手 (00243717)
TAMAI Kenichiro  Tokushima University School of Dentistry Research Associate, 歯学部, 助手 (90243716)
SATOMURA Kazuhito  Tokushima University School of Dentistry Research Associate, 歯学部, 助手 (80243715)
GOTOH Yuji  Tokushima University School of Dentistry Research Associate, 歯学部, 助手 (60225670)
RIKIMARU Koichi  Tokushima University School of Dentistry Associate Professor, 歯学部, 助教授 (40220800)
滝下 幸夫  徳島大学, 歯学部, 助手 (30226964)
平岩 清貴  徳島大学, 歯学部附属病院, 講師 (30173214)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1992: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1991: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsHuman Osteoblast / Bone Formation / Mineralization / PDGF / Osteopetrosis / Bone Marrow Stromal Cell / chondrocyte / 石炭化 / 骨芽細胞 / Mineralized Nodule / サイトカイン / リン酸化 / 遺伝子発現調節
Research Abstract

We investigated the effect of PDGF on growth stimulation in human osteoblastic cells. PDGF stimulated the proliferation of these cells and this mechanism was mediated by tyrosin kinase activity of the PDGFrecepter followed by phosphorylation of cellular proteins. We are also studying the induction of gene expression by PDGF in these cells.
Biological bone formation system is necessary for studying on a role of osteoblast during bone remodeling. Osteoblasts derived from human bone were injected in diffusion chamber and implanted intraperitoneally into nude mouse. After 6-8 weeks, calcified body was formed in the cell layer touched membrane filter. Ultrastructually, we showed this calcified body had the similar process to bone formation in vivo.
Bone marrow tissue is known to contain the cells which can differentiate to the osteogenic direction. On this culture system, mineralized nodules which embedded osteoblasts and osteocytes were formed. By electron-microscopic study, this mineralization was considered to be initiated in association with the matrix vesicles and to progress along the collagen fibrils.
Osteopetrosis, characterized by a systemic osteosclerosis, has been given attention to the osteoclast differentiation and bone remodering. We studied the structures of the mandible of a man who had osteopetrosis. The bone marrow cavity was almost occupied by sclerotic bone with very irregular lamination. There was no sign of normal bone remodeling in cortical bone.
Not only osteoblast but also chondrocyte concerns the skeletal growth. Using terminal differentiated chondrocyte, we intestigated how retionic acid stimulates their proliferation and induces dedifferentiation. From our experiments, we suggested that protein kinase C activity mediates the effect of retinoic acid in this chondrocyte.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Fumihiro Matsumoto: "Light and electron-microscopic observations on the manbibular condylar cartilages in growing rats on a low-calcium diet." Acta Anatomica. 142. 41-48 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kazuhito Satomura: "Mineralized nodule formation in rat bone marrow stromal cell culture without β-glycerophosphate." Bone adn Mineral. 14. 41-54 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kazuhito Satomura: "Ultrastructure of minaralized noduled formed in rat bone marrow stromal cell culture in vitro" Acta Anatomica. 142. 97-104 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 玉井 健一郎: "培養軟骨細胞におけるレチノイン酸作用機構へのプロテインキナーゼCの関与について" 四国歯学会雑誌. 5. 77-88 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 中西 宏彰: "ヒト顎骨由来骨芽細胞様細胞の増殖におよぼす血小板由来成長因子の影響" 四国歯学会雑誌. 5. 107-119 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Fumihiro Matsumoto: "Light and electron-microscopic observations on the mandibular condylar cartilages in growing rats on a low-calcium diet" Acta Anatomica. 142. 41-48 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kazuhito Satomura: "Mineralized nodule formation in rat bone marrow stromal cell culture without beta-glycerophosphate" Bone and Mineral. 14. 41-54 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kazuhito Satomura: "Ultrastructure of mineralized nodules formed in rat bone marrow stromal cell culture in vitro" Acta Anatomica. 142. 97-104 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kenichiro Tamai: "Involvement of protein kinase C on proliferation and dedifferentiation of retinoic acid -treated cultured chondrocyte" Shikoku Dental Research. 5. 77-88 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hiroaki Nakanishi: "Effect of platelet-derived growth factor on the proliferation of cultured human osteoblastic cells" Shikoku Dental Research. 5. 107-119 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 玉井 健一郎: "培養軟骨細胞におけるレチノイン酸作用機構へのプロテインキナーゼCの関与について" 四国歯学会雑誌. 5. 77-88 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kazuhito SATOMURA: "Mineralized nodule formation in rat bone marrow stromal cell culture without βーglycerophosphate" Bone and Mineral. 14. 41-54 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kazuhito SATOMURA: "Ultrastructure of Mineralized Nodules Formed in Rat Bone Marrow Stromal Cell Culture in vitro" Acta Anatomica. 142. 97-104 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 中西 宏彰: "ヒト顎骨由来骨芽細胞様細胞の増殖におよぼす血小板由来成長因子の影響" 四国歯学会雑誌.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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