| Project/Area Number |
03454492
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SATOH Masamichi Kyoto University, Facl. Pham. Sci., Professor, 薬学部, 教授 (80025709)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Shuji Kyoto University, Facl. Pharm. Sci., Assoc. Prof., 薬学部, 助教授 (60177516)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥6,400,000 (Direct Cost: ¥6,400,000)
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| Keywords | Xenopus oocyte / mRNA / antisense DNA / G-protein / GTP-binding / muscarinic receptor / serotonin receptor / hybrid arrest / ムスカリンレセプタ- / セロトニンレセプタ- / ホスホリパ-ゼC / ラット脳 |
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| Research Abstract |
To Assign GTP-binding protein (G-protein) subtyps involved in the signal transduction from exogenous receptors to phospholipase C in the Xenopus oocyte translation system, antisense DNA complementary to rat G-protein alpha-subunit mRNA was designed and injected together with rat brain poly(A)^+mRNA. Current responses of mRNA-injected oocytes to acetylcholine (ACh) was suppressed by a co-injection of Gilalpha-antisense DNA dose-dependently, but response of the same oocytes to serotonin (5-HT) was not inhibited. In the oocytes co-injected with Goalpha-antisense DNA, the 5-HT response was more effectively suppressed than the ACh response. These result suggest that Goalpha but not Gilalpha intermediates brain 5-HTlc receptor function, and in contrast, muscarinic receptors derived from rat brain utilize Gilalpha rather than Goalpha to activate phospholipase C. Putative effects of G-protein on voltage-dependent calcium channels were also suggested.
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