| Project/Area Number |
03454494
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Kumamoto University |
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Principal Investigator |
FURUISHI Kazuchika (1992) Kumamoto Univ., Pharmaceutical Sciences, Assistant Professor, 薬学部, 助手 (40238663)
久保田 幸穂 (1991) 熊本大学, 薬学部, 教授 (30040299)
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| Co-Investigator(Kenkyū-buntansha) |
FUNAKOSHI Takayuki Kumamoto Univ., Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (90150549)
SHOJI Shozo Kumamoto Univ., Pharmaceutical Sciences, Professor, 薬学部, 教授 (60040317)
古石 和親 熊本大学, 薬学部, 助手 (40238663)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1991: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Myristoylation / AIDS / HIV-1 / p17^<gag> / Precursor 55^<gag> / N-Myr-Gly / p17^<8α8> / Pr55^<8α8> / HIVー1 / レトロウイルス / Nーミリストイルグリシン / 抗Nーミリストイルグリシン抗体 |
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| Research Abstract |
Novel antibodies were raised against a synthetic NH_2-terminal myristoyl (N-Myr-) glycine that is characteristic of all N-myristoylated proteins. Antibody raised against N-Myr-Gly-hemocyanin reacted specifically with N-Myr-Gly moiety of N-Myr-Gly-protein. The immunoreaction was competed by hemocyanin as well as albumin conjugated with N-Myr-Gly, while underivatized proteins or various fatty acids except myristic acid had no effect. The antibody specifically reacted with the N-myristoylated p17^<gag> and precursor 55^<gag> of the HIV-1 infected cell lysate. Therefore, the antibody is shown to be very useful tool for resolution of HIV-1 virus life cycle.
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