| Project/Area Number |
03454545
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
代謝生物化学
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
FUJIMOTO Daisaburo Tokyo Univ. of Arg. Tech., Professor, 農学部, 教授 (40004288)
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| Co-Investigator(Kenkyū-buntansha) |
SAKANO Yoshiyuki Tokyo Univ. of Arg. Tech., Professor, 農学部, 教授 (70014959)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | Immunoglobuln / Tumour cell / Natural antibody / beta-galactoside / がん / α-ガラクトシド / 抗ーαーガラクトシル基 / 抗βーガラクトシル基 |
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| Research Abstract |
A kind of immunoglobulin M was isolated from normal human serum by affinity chromatography on an elastin-Sepharose column. This immunoglobulin M has a tumouricidal activity in combination with an glutamine analogue, DON.
A kind of immunoglobulin G with anti-beta-galactosyl specificity was isolated from normal human serum by affinity chromatography on an lactose-Sepharose column. The sugar specificity of this immunoglobulin was found to be different from that of the known anti-galactosyl antibodies, indicating that this immunoglobullin G was developed, and the concentration of this immunoglobulin G in sera from 7 cancer patients and 50 normal subjects were determined. The concentration in the serum from one cancer patients was found to be markedly high as compared with that of normal subject. Since expression of the terminal beta-galactosyl groups on the cell surface is closely associated with metastatic ability of tumor cell, this immunoglobulin might have potential in various fields of cancer research.
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