| Project/Area Number |
03454560
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
NISHIMOTO Takeharu Kyushu University, Graduate School of Medical Science, Professor, 大学院医学系研究科, 教授 (10037426)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1993: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1991: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | CELL CYCLE / CCG1 / RCC1 / RPS4X / DAD1 / CDC25 / PCC / G2-BLOCK / 温度感受性変異 / DADl / CCGl / 温度感受性変異株 / CCG2(RPS4X) / アポトーシス / CCG2 |
|---|
| Research Abstract |
We have isolated temperature sensitive mutants for cell growth from the BHK21 cell lines derived from golden hamsters. Using these mutants as a receipient of DNA-mediated gene transfer, we have been cloning human genes complementing ts mutation. So far, we have cloned human genes, RCC1, CCG1, RPS4X and DAD1. Under the present research project, we have cloned the RPS4X and DAD1 genes, and characterized the function of RCC1 and CCG1 gene-products. Additionaly, we have proved that the human cDNA encoding ubiquitin-activating enzyme E1 complements tsBN75, a temperature sensitive mutant of the BHK21 cell line which shows G2 block at restrictive temperature.
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