Construction and expression of gene families

• Project/Area Number: 03454561
• Research Category: Grant-in-Aid for General Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: 分子遺伝学・分子生理学
• Research Institution: Fujita Health University
• Principal Investigator: KUROSAWA Yoshikazu Fujita Health Univ., Comprehensive Med. Sci., Professor, 総合医科学研究所, 教授 (10109259)
• Co-Investigator(Kenkyū-buntansha): NAITO Kazuhiro Fujita Health Univ. College, , Assistant, 助手 (80132257) ; OKAMURA Kazuhiko Fujita Health Univ. College, , Assistant, 助手 (60132255) ; MATSUI Toshikazu Fujita Health Univ., Comprehensive Med. Sci., Assistant Professor, 医学部, 講師 (50181681) ; HASHIMOTO Keiichiro Fujita Health Univ., Comprehensive Med. Sci., Assistant Professor, 総合医科学研究所, 講師 (70192268) ; 黒沢 良和 藤田保健衛生大学, 総合医科学研究所, 教授 (10109259)
• Project Period (FY): 1991 – 1992
• Project Status: Completed (Fiscal Year 1992)
• Budget Amount: ¥6,400,000 (Direct Cost: ¥6,400,000); Fiscal Year 1992: ¥2,300,000 (Direct Cost: ¥2,300,000); Fiscal Year 1991: ¥4,100,000 (Direct Cost: ¥4,100,000)
• Keywords: MHC / Complement / Immune system / Homeobox / Sponge / 抗体 / 円口類 / 補体第二経路 / 進化

Research Abstract

It has been believed that the most primitive animal that produces antibodies is cyclostome. We isolated the gene which encodes hagfish "antibodies" and determined the nucleotide sequence. Surprisingly, it turned out to be a gene encoding not immunoglobulin but complement C3. We developed a new method using PCR for isolation of MHC genes from primitive vertebrates. By this method, we have succeeded to isolate genes encoding MHC classI and classIIbeta from carp and also a gene encoding MHC classI from shark. We also showed that at the level of bony fish MHC classI genes have been expanded to at least several tens and their sequences are quite heterogeneous.
We examined homeobox-containing genes from various phyla of animals. We isolated two homeobox genes, prox1 and prox2 from sponge. The structure of prox1 and prox2 indicated that they have gained the properties characteristics for each class of homeobox genes isolated from higher animals. We isolated four homeobox genes from hydra. A new kind of homeobox gene was identified from C. elegans. Two kinds of new homeobox genes, GBX1 and GBX2, were isolated from human. We mapped GBX1 to chromosome 7q36.1.

Research Products

• [Publications] K.Hashimoto: "Identification of a shark sequcnce resembling the major histocompatibility complex class I α3 domain." Proc.Natl.Acad.Sci.USA. 89. 2209-2212 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] H.Ishiguro: "Isolation of hagfish genes encoding a complement component." EMBO J.11. 829-837 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M.Naito: "Identification of a homeobox-containing gene located between lin-45 and unc-24 at chromosome IV in the Cacnorhabditis clcgans." Nucl.Acids Rcs.20. 2967-2969 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K.Okamura: "Expansion of genes the encode major histocompatibility complex class I-like molecules in cyprinid fishes." J.Immunology.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M.Scimiya: "Homcobox-containing genes in the most primitigve mctazoan spongc."
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] T.Matsui: "Expression of a novel human homeobox-containing gene mapped to chromosome 7q36.1 in hematopoictic cclls."
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K. Hashimoto: "Identification of a shark sequence resembling the major histocompatibility complex class I alpha3 domain." Proc. Natl. Acad. Sci. USA. 89. 2209-2212 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] H. Ishiguro: "Isolation of hagfish genes encoding a complement component." EMBO J.11. 829-837 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] M. Naito: "Identification of a homeobox-containing gene located between lin-45 and unc-24 at chromosome IV in the nematode Caenorhabditis elegans." Nuc. Acids Res.20. 2967-2969 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K. Okamura: "Expansion of genes that encode major histocompatibility complex class I-like moleucles in cyprinid fishes." Immunology.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] M. Seimiya: "Homeobox-containing genes in the most primitive metazoan sponge."
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] T. Matsui: "Expression of a novel human homeobox-containing gene mapped to chromosome 7q36.1 in hematopoietic cells."
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K.Hashimoto: "Idenification of a shark sequence resembing the major histocompatiblity complex class I α3 domain." Proc.Natl.Acad.Sci.USA. 89. 2209-2212 (1992)
• [Publications] H.Ishiguro: "Isolation of hagfish genes encoding a complement component." EMBO J.11. 829-837 (1992)
• [Publications] M.Naito: "Identification of a homeobox-containing gene located between lin-45 and unc-24 at chromosome IV in the nematode Caenorhabditis elegans." Nucl.Acids Res.20. 2967-2969 (1992)
• [Publications] K.Okamura: "Expansion of genes that encode major histocompatibility complex classI-like molecules in cyprinid fishes." J.Immunology.
• [Publications] M.Seimiya: "Homeobox-containing genes in the most primitigve metazoan sponge."
• [Publications] T.Matsui: "Expression of a novel human homeobox-containing gene mapped to chromosome 7q36.1 in hematopoietic cells."
• [Publications] K.Hashimoto: "Identification of a shark sequence resembling the major histocompatibility complex class I α3 domain." Proc.Natl.Acad.Sci.USA.
• [Publications] H.Ishiguro: "Isolation of hagfish genes encoding a complement component." EMBOJ.11. 829-837 (1992)
• [Publications] M.Naito: "Identification of a homebox-containing gene between lin-45 and unc-24 at chromosome IV in the nematode Caenorhabditis elegans." Nuc.Acids Res.
• [Publications] K.Okamura: "Comparison of nucleotide sequences from upstream of DQ52 gene to Sμ region of immunoglobulin heavy chain gene loci between Suncus murinus,mouse and human." Nucl.Acids Res.
• [Publications] K.Hashimoto: "Evolution of MHC domains:Strategy for isolation of MHC genes from primitive animals.Molecular evolution of the major histocompatibility complex." Springer-Verlag, (1992)