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Molecular architecture of light-harvesting complexes of chromophytes

Research Project

Project/Area Number 03455014
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KATOH Tetzuya  Dept.Botany, Fac.Sci., Kyoto Univ., Ass.Prof., 大学院・理学研究科, 助教授 (30025308)

Co-Investigator(Kenkyū-buntansha) TANAKA Ayumi  Dept.Botany, Fac.Sci., Kyoto Univ., Lecturer, 大学院・理学研究科, 講師 (10197402)
MIMURO Mamoru  National Institute for Basic Biology, Assistant, 基礎生物学研究所, 助手 (40142004)
辻 英夫  京都大学, 理学部, 教授 (20025323)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥4,400,000 (Direct Cost: ¥4,400,000)
KeywordsCarotenoid / Fucoxanthin / light-harvesting complexes / Brown algae / Chromophyte / Excitation Energy Transfer / Fucoxanthin Chlorophyll Protein / フコキサンチン / カロテノイド / 光捕捉 / 光合成 / 励起エネルギー / フコキサンチン・クロロフィル複合体 / 光捕捉蛋白 / エネルギー転移 / エネルギ-転移
Research Abstract

Fucoxanthin-Chl a/c protein (FCP), the major light harvesting protein of brown algae and diatoms, is very unstable and its excitation energy transfer between pigments was very readily injured when exposed to detergents conventionally used for isolating various photoactive protens from green plants. We studied on the conditions of FCP isolation from the thalli of brown algae, and revealed that the assemblies of FCP are bound with photochemical reaction center to form complexes which are embedded in thylakoids, like LHCP in green algae. Monomeric form of FCP consisted of a protein with MW 20,226 (in the case of Ectocarpus), 4 Chl a, 1 Chl c and 4 fucoxanthin, and its native form showed an highly efficient energy transfer (higher than 95%) between fucoxanthin*Chl a, Chl c*Chl a. Fucoxanthin bound in FCP showed more red-shifted absorbance than liberated one, showing an absorbance spectrum similar to that dissoved in imidoN-containing, polarsolvets. Exposed to warm temperature (40゚C), the red-shifted FCP turned to green reflecting the blue-shift of fucoxanthin with concomitant loss of CD signals, indicating the disarrangement pigment molecules. However, the resonance Raman spectrum of blue-shifted FCP stayd unaltered from the native FCP,suggesting that fucoxanthin active in excitation energy transfer is highly interacted with Chl a in FCP,and this interaction is responsible to the red shift of fucoxanthin.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report

Research Products

(52 results)

All Other

All Publications (52 results)

  • [Publications] T.Katoh: "Fluorescence of allenic carolenoid,fucoxanthin, its implication to energy transfer in photosynthelic pigment system." Photosynthesis Res.27. 221-226 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Mimuro: "Fluorescence properties ofallenic carotenoid,fucoxanthin.Analysis of the effect of keto carbonyl group by using a model compound,all-trans-β-apo-8′carotenal." J.Luminescence. 51. 1-10 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Nagae: "Calculation of the excitation transfer matrix elements between the state of carotenoid and the S2 or S1 state of bacteriochlorophyll." J.Chem Phys.98. 8012-8023 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Mimuro: "Direct measurement of the low-lying singlet excited (2′Ag) state of a linear carotenoid,neurosporene,in solution." Chemical Physice Letters. 204. 101-105 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Mimuro: "The effect of molecular structure on the relaxation processes of carotenoids containing a carbonyl group." Chemical Physice Letters. 213. 576-580 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Ogata: "A novel peridnin-chorophyll a protein (PCP) from the marine dinoflagellate,Alexandrimm cohorticula : a high pigment content and plural spectral forms of peridinin and chlorophylla." FEBS Letters. 356. 367-371 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M,Mimuro: "Molecular structure and optical properties of carotenoids for the in vivo 271-energy transfer function in the algal photosynthetic pigment system." Biochim. Biophys. Acta. 1098. 274- (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M,Mimuro: "Quantitative analysis of the solvent effect on the relaxation processes of carotenoids showing dual emissive characteristics." Chemical Physics Letters. 191. 219-224 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tanaka,Y: "Stabilization of apoproteins of light-harvesting chlorophyll a/b-protein complexes by fccding 5-aminolevulinic ncid under intermittent illumination." Plant Physiol. Biochem. 30. 123-128 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mimuro,M: "Mnalysis of the excitation energy transfer processes from carotenoids to chlorophyll a in algal photosynthesis pigment systems." Photochem. Photobiol. 55. 125- (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Katoh,T: "Xanthosomes : suptamolecular assemblics of xanthophyll-chlorophyll a/c protein complexcs." Methods in Enzymology Carotenoids Part B,Academic Press. 214. 402-412 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tanaka,Y: "Preferential accumulation of apoproteins of the light harvesting chlorophyll a/b protein complex in greening barley leaves treated with 5-aminolevulinic acid." Planta. 192. 92-97 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Katoh,T: "Fucoxanthin- chlorophyll a/c protein in brown algae: their molecular assembly and energy transfer." Frontiers of Photobiology Excerpta Medica(Netherlands). 21-24 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Katoh: "Frontiers of Photobiology" Excerpta Medica(Netherlands),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 加藤哲也: "海洋生物のカロテノイド代謝と生物活性" 日本水産学会,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Katoh, T., Nagashima, U., & Minoru, M.: "Fluorescence properiteis of the allenic carotenoid fucoxanthin : Implication for energy transfer in photosynthetic pigment systems" Photosynthesis Research. 27. 221-226 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mimuro, M., Nagashima, U., Takaichi, S., Nishimura, I., Yamazaki, I.& Katoh, T.: "Molecular structure and optical properties of carotenoids for the in vivo 271-energy transfer function in the algal photosynthetic pigment system." Biochim.Biophys.Acta. 1098. 274 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mimuro, M., Nishimura, I., Yamazaki, I., Katoh, T.& Nagashima, U.: "Fluorescence properties of the allenic carotenoid fucoxanthin. Analysis of the effect of keto carbonyl group by using a model compound, all-trans-b-apo-8' carotenal." J.Luminescence. 51. 1-10 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mimuro, M., Nagashima, U., Nagaoka, S., Takaichi, S., Yamazaki, I., Nishimura, I.& Katoh, T.: "Quantitative analysis of the solvent effect on the relaxation processes of carotenoids showing dual emissive characteristics." Chemical Physics Letters. 191. 219-224 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tanaka, Y., Tanaka, A.& Tsuji, H.: "abilization of apoproteins of light -harvesting chlorophyll a/b-protein complexes by feeding 5-aminolevulinic and under intermittent illumination." Plant Physiol. Biochem. 30. 123-128 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mimuro, M., & Katoh, T.: "Analysis of the excitation energy transfer processes from carotenoids to chlorophyll a in algal photosynthesis pigment systems." Photochem. Photobiol. 55. 125 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mimuro, M., Nagashima, U., Nagaoka, S., Takaichi, S., Yamazaki, I., Nishimura, I.& Katoh, T.: "Direct measurement of the low-lying singlet excited (2'Ag) state of a linear carotenoid, neurosporene, in solution." Chemical Physics Letters. 204. 101-105 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nagae, H., Kakitani, T., Katoh, T,& Mimuro, M.: "Calculation of the excitation transfer matrix elements between the state of carotenoid and the S2 or S1 state of bacteriochlorophyll." J.Chem Phys.98. 8012-8023 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mimuro, M., Nishimura, Y., Takaichi, S., Yamano, Y., Ito, M., Nagaoka, S., Yamazaki, I., Katoh, T.Nagashima, U: "The effect of molecular structure on the relaxation processes of rotenoids containing a carbonyl group." Chem.Phys.Letters. 213. 576-580 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ogata, T., Kodama, M., Nomura, S., Kobayashi., Nozawa, T., Katoh, T & Mimuro, M.: "A novel peridinin-chlorophyll a protein (PCP) from the marine dinoflagellate, Alexandrium cohorticula : a high pigment content and plural spectral forms of peridinin and chlorophyll a." FEBS Letters. 356. 367-371 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tanaka, A.Tanaka, Y.and Tsuji, H.: "Preferential accumulation of apoproteins of the ligh harvesting chlorophyll a/b protein complex in greening barley leaves treated with 5-aminolevulinic acid." Planta. 192. 92-97 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Katoh, T: "Fucoxanthin-chlorophyll a/c protein in brown algae : their molecular assembly and energy transfer." Frontiers of Photobiology Excerpta Medica (Netherlands). 21-24 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Katoh, T., Tanaka, A.& Mimuro, M.: "Xanthosomes : supramolecular assemblies of xanthophyll-chlorophyll a/c protein complexes." Methods in Enzymology Vol.214 Carotenoids Part B,Academic Press. 402-412

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Katoh: Frontiers of Photoboology. Excerpta Medica (Netherlands),

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Katoh: Biological activities of carotenoids physiological function in algae.ed.Japanese Society of ary Science, 87-96 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Mimuro: "Quantitative analysis of the solvent effect on the relaxation processes of carotenoids showing dual emissive characteristics." Chemical Physics Letter. 191. 219-224 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Mimuro: "Direct measurement of the low-lying singlet excited(2^1Ag)state of a linear carotenoid,neurosporene,in solution." Chemical Physics Letter. 204. 101-105 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Nagae: "Calculation of the excitation transfer matrix elements between the state of carotenoid and the S_2 or S_1 state of bacteriochlorophyll." J.Chemical Physics.98. 8012-8023 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Mimuro: "The effect of molecular structure on the relaxation processes of carotenoids containing a carbonyl group." Chemical Physics Letters. 213. 576-580 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] A.Tanaka: "Preferential accumulation of apoproteins of the light harvesting chlorophyll a/b-protein complex in greening barley leaves treated with 5-aminolevulinic acid" Planta. 192. 92-97 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Ito: "Conversion of chlorophyll b to chlorophyll a by isolated cucumber etioplasts" Archives of Biochem.Biophys. 306. 148-151 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Katoh: "Research in Photosynthesis." Kluwer Academic Publishers,Dordrecht.The Netherlands, 4 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Katoh: "Frontiers of Photobiology." Excerpta Medica.Amsterdam,The Netherlands, 4 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Katoh,T.: "Spectral form of fucoxanthin involved in energy transfer to chlorophyll α in the light-harvesting proteins of brown algae" Proceeding of 9th International Congress on Photosynthesis,Nagoya,Aug 1992(in press),to be published from Kluwer Academic Publishers,The Netherlands(1993).

    • Related Report
      1992 Annual Research Report
  • [Publications] Katoh,T.: "Fucoxanthin-chlorophyll a/c protein in brown algae:their molecular assembly and energy transfer" Proceeding of 11th International Congress on Photobiology,Kyoto,Sept.1992(in press),to be published from Elsevier Publishers,Amsterdam (1993).

    • Related Report
      1992 Annual Research Report
  • [Publications] Nagae,H.: "Theoretical study on the interaction between carotenoid and bacteriochlorophyll. Calculations of the excitation transfer matrix elements between the S_2 or S_1 state of neurosporene and the S_2 or S_1 state of bateriochlorophyll α" J.Chem.Physics(in press).

    • Related Report
      1992 Annual Research Report
  • [Publications] Mimuro,M.: "Molecular structure and optical properties of carotenoids for the in vivo energy transfer function in the algal photosynthetic pigment system." Biochim.Biophys.Acta.1098. 271-274 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] "Quantiative analysis of the solvent effect on the relaxation processes of carotenoids showing dual emissive characteristics." Chemical Physics Letter. 191. 219-224 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 加藤 哲也: "褐藻の光捕捉装置"

    • Related Report
      1992 Annual Research Report
  • [Publications] Katoh,T.: "Methods in Enzymology,Vol.214,Carotenoids(ed.L.Packer)" Academic Press(New York),

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Mimuro: "Carotenoids in photosynthesis:absorption,transfer and dissipation of light energy" Pure&Appl.Chem.63. 123-130 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Katoh: "Fluorescence of allenic carotenoid,fucoxathin,its implication to energy transfer in photosynthetic pigment system" Photosynthesis Res.27. 221-226 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Mimuro: "Fluorescence properties of the allenic carotenoid fucoxanthin:Analysis of the effect of keto carbonyl group by using a model compound,allーtransーapoー8'ーcarotenal" J.Luminescence.

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Mimuro: "Molecular structure and optical properties of carotenoids for the in vivo energy transfer function in the algal photosynthetic pigment system" Biochim.Biophys.Acta. 1098. 271-274 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Mimuro: "A special molecular structure in carotenoids is necessary for the in vivo energy transfer function in algal photosynthetic pigment system" Biochim.Biophys.Acta.

    • Related Report
      1991 Annual Research Report
  • [Publications] 加藤 哲也: "褐藻の光合成光捕捉装置" Plant Science Tomorrow.

    • Related Report
      1991 Annual Research Report
  • [Publications] T.katoh: "Methods Enzymology,Vol.110,Carotenoids." Academic Press,New York,

    • Related Report
      1991 Annual Research Report

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Published: 1991-03-31   Modified: 2016-04-21  

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