IDENTIFICATION OF STAINS FROM MEDICO LEGAL ASPECTS

• Project/Area Number: 03455017
• Research Category: Grant-in-Aid for General Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: 広領域
• Research Institution: KYUSHU UNIVERSITY
• Principal Investigator: NAGATA Takeaki Kyushu Univ., Fac. of Med., Professor, 医学部, 教授 (00078586)
• Co-Investigator(Kenkyū-buntansha): MIYAJIMA Ikuo Kyushu Univ., Fac. of Agri., Assistant, 農学部, 助手 (20182024) ; URAKAWA Narumi Kyushu Univ., Fac. of Med., Assistant, 医学部, 助手 (80232916) ; IMAMURA Tohru Kyushu Univ., Fac. of Med., Assistant, 医学部, 助手 (00193681) ; KUDO Keiko Kyushu Univ., Fac. of Med., Assistant, 医学部, 助手 (10186405) ; KIMURA Kojiro Kyushu Univ., Fac. of Med., Assistant Professor, 医学部, 講師 (30153191)
• Project Period (FY): 1991 – 1992
• Project Status: Completed (Fiscal Year 1992)
• Budget Amount: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
• Keywords: forensic science / criminology / phytochemistry / high performance liquid chromatography / flavonoids / stains / blade of dried grass / 高速液体クロマトグラフィ-

Research Abstract

A study was designed to identify stains derived from plant pigments. Using high performance liquid chromatography (HPLC), 13 species of common weeds were examined, and 12 flavonoids were detected from as low as 100 to 200 ug of dried leaves and from their stains. Identification of plant species should be made, based on the various chromatographic patterns of the flavonoids with different retention indicies, using rutin and rhamnetin as the reference standards.
The established method was applied to three crime cases. Stains on clothing from a victim or a suspect which were later on determined as grass stains, and a blade of dried grass taken from another were examined to identify the sites of crimes. The species of each sample was identified based on the characteristic distribution pattern of flavonoids seen on high pergormance liquid chromatography. The scene of the crime was clearly traced from the evidential material and this method was proved to be useful for the criminological application.

Research Products

• [Publications] Nagata T.: "Identfication of species of weeds using High Performance Liguid Chromtography in three crime cases" International Journal of Legal Medicine. 104. 285-287 (1991)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] NAGATA T: "Identification of species of weeds using high performance liquid chromatography in three crime cases" Int. J. Legal Med. 104. 285-287 (1991)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nagata T.: "Identification of species of weeds using High Performance Liguid Chromatography in three crime cases" International Journal of Legal Medicine. 104. 285-287 (1991)