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Development of superbioreactors

Research Project

Project/Area Number 03555179
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 有機工業化学
Research InstitutionKyushu Institute of Technology

Principal Investigator

KONDO Hiroki  Department of Biochemical Engineering and Science, Kyushu Institute of Technology, professor, 情報工学部, 教授 (60038057)

Co-Investigator(Kenkyū-buntansha) KURASHIGE Jun  Ajinomoto Corp.,vice manager, 中央研究所油脂研究所, 副部長
SONOMOTO Kenji  Department of Biochemical Engineering and Science, associate professor, 情報工学部, 助教授 (10154717)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 1992: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1991: ¥9,200,000 (Direct Cost: ¥9,200,000)
Keywordsorganic solvent systems / chemical modification of enzymes / lipases / conversion of enzyme functions / organic silicon compounds / stereoselective esterification / support-free bioreactors / tubular / 酵素機能交換 / リパ-ゼ / 光学分割 / テルペンアルコ-ル / 2-(4-クロロフェノキシ)プロピオン酸 / トリメチルシリルメタノ-ル / 有機溶媒 / ケイ素置換反応
Research Abstract

A.Development of catalytic devices (1)Conversion of enzyme functions by chemical modification: Upon chemical modification of tyrosine residues of a Pseudomonaslipase, its esterase activity was reduced drastically, while lipase activity was little affected. This phenomenon was observed when two of the tyrosine residues of this enzyme were modified. (2) Search of biocatalysts effective for transformation of unnatural compounds: Enzymatic optical resolution of 2-trimethylsily(TMS)-1- propanol(A),1-TMS-2-propanol(B) and 1-TMS-1-propanol(C) was attempted. Unlike chemical methods, enzymatic methods were found to be superior for esterifying A and B with 5-phenylvaleric acid. Furthermore, efficient optical resolution was achieved for B with Pseudomonas sp. lipoprotein lipase. Because of a steric bulk, C reacted more slowly than A and B. but a significant stereoselectivity was observed for C as well.
B.Development of support-free biocatalyst
Stereoselective esterification of dl-2-(4-chlorophenoxy) propionic acid with myristyl alcohol was carried out in water-saturated carbon tetrachloride-issoctanol (8:2) in the presence of yeast lipase OF360 at 30゚C. A 3-fold higher rate and high stereoselectivity was observed for enzyme powder than that for celite-adsorbed enzyme. As an application a continuous reactor was developed in which a glass column was filled with enzyme powder. This system yielded a 4-fold higher productivity than the celite-adsorbed enzyme.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Shigeori TAKENAKA: "Interaction of novel tris-intercalators with DNA.Spectroflucrometric studies." Nucleic Acids Res.,Symposium Series. 71-72 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Masaya KAWASE: "Inspection of an acyl-enzyme intermediate in a lipase reaction by gas chromatography-mass spectrometry and modeling of the reaction mechanism." Biocatalysis. 6. 43-50 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kenji SONOMOTO: "Enzymatic conversion of artificial organosilicon compounds by alcohol dehydrogenase." Enzyme Microb.Technol.14. 640-643 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kenji SONOMOTO: "Effects of sila-substitution of substrates on activity of alcohol dehydrogenase." Ann.N.Y.Acad.Sci.672. 66-70 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Chang-Sheng Rui: "Amperometric assay of creatinine in urine by flow injection analysis based on conjugated reactions of immobilized enzymes.Simultaneous compensation of endogenous ammonia." Ann.N.Y.Acad.Sci.672. 264-271 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shigeori TAKENAKA: "Synthesis and characterization of novel tris-intercalators having potentially two different DNA binding modes." Supramol.Chem.(1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 田中 渥夫: "バイオリアクターの世界-実践者のためのその基礎と応用-" ハリオ研究所, 329 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shigeori Takenaka et al.: "Synthesis of characterization of novel tris-intecaltors having potentially two different DNA binding modes" Supramol.Chem. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Chang-Sheng Ruo et al.: "Amperometric assay of creation in urine by flow injection analysis based on conjugated reactions of immobilized enzymes. Simulataneous compensation of endogenou ammonia" Ann.N.Y.Acad.Sci.672,264 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kenji Sonomoto et al.: "Effects of sila-substitution of substrates on activity of alcohol dehydrogenase" Ann N.Y.Acad Sci.672,66 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kenji Sonomoto et al.: "Enzymatic conversion of artificial organosilicon compounds by alcohol dehydrogenase" Enzyme Microb.Technol. 14,640 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Masaya Kawase et al.: "Inspection of an acyl-enzyme intermediate in a lipase reaction by gas chromatography-mass spectrometry and modeling of the reaction mechanism,Biocatalysis" 6,43 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shigeori Takenaka et al.: "Interaction of novel tris-intercalators with DNA. Spectroscopic studies" Nucleic Acids, Symposium Series. 71 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shigeori Takenaka: "Interaction of novel tris-intercalators with DAN,Spectroflucromctric studis." Nucleic Acids Res.,Symposium Seris. 71-72 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Masaya Kawase: "Inspcction of an acyl-enzyme intermediate in a lipase reaction by gas Chromatography-mass spcctrometry and modeling of the reaction mechanism." Biocatalysis. 6. 43-50 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kenji Sonomoto: "Enzymatic conversion of artificial organosilicon compounds by alcohol dehydrogenase." Enzyme Microb.Technol.14. 640-643 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kenji Sonomoto: "Effects of sila-substitution of sudstrates on activity of alcohol Dehydrogenase." Ann.N.Y.Acad.Sci.672. 66-70 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Chang-Sheng Rui: "Amperometric assay of creatinine in urine by flow injection analysis based on conjugated reactions of immobilized enzymes.Simultaneous compensation of endogenous ammonia." Ann.N.Y.Acad.Sci.672. 264-271 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shigeori Takenaka: "Synthesis and characterization of novel tris intercalators having potentially two different DNA binding modes." Supramol.Chem.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 田中 渥夫: "バイオリアクターの世界ー実践者のためのその基礎の応用ー" ハリオ研究所, 329 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hiroki Kondo: "Acetyl-CoA carboxylase from Escherichia coli:Gene organization and nucleotide sequence of the biotin carboxylase subunit." Proc.Natl.Acad.Sci.USA. 88. 9730-9733 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiroki Kondo: " ^<15>N NMR studies on aspartate aminotransferase reconstituted with [ ^<15>N] PLP" Enzymes dependent on pyridoxal phosphate and other carbonyl compounds as cofactors. 145-147 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tadashi Tanabe: "Structure,reaction and molecular evolution of biotin carboxylase." Enzymes dependent on pyridoxal phosphate and other carbonyl compounds as cofactors. 637-639 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takuo Kawamoto: "Efficient optical resolution of 2-(4-chlorophenoxy)propanoic acid with lipase by the use of organosilicon compounds as substrate:the role of silicon atom in enzymatic recognition." J.Biotechnol.18. 85-92 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Toshiaki Fukui: "Construction of a non-support bioreactor:optical resolution of 2-(4-chlorophenoxy)propanoic acid in an organic solvent system." Appl.Microbiol.Biotechnol.35. 563-567 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Gerhard Mittenhuber: "Identification of the DNA region responsible for sulfur-oxidizing ability of Thiosphaera pantotropha." J.Bacteriol.173. 7340-7344 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 園元 謙二: "バイオリアクタ-の世界" 柴田ハリオ硝子, (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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