Functional expression and utilization of bacterial cytochrome C__-
| Project/Area Number |
03556010
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用微生物学・発酵学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KODAMA Tohru The university of Tokyo, Department of Agriculture, Professor, 農学部, 教授 (30011901)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBANO Yuzi Suntory, Institute for fundamental research, Head manager, 基礎研究所微生物科学研究室, 室長
MIYAWAKI Osato The university of Tokyo Department of Agriculture, Associate professor, 農学部, 助教授 (80012053)
天知 輝夫 サントリー(株), 基礎研究所, 所長
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥15,800,000 (Direct Cost: ¥15,800,000)
Fiscal Year 1992: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1991: ¥11,900,000 (Direct Cost: ¥11,900,000)
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| Keywords | cytochrome C__- / electro chemistry / シトロクロムC / 電気化学反応 |
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| Research Abstract |
Cytochrome c__-_<552> gene was cloned from the total DNA of the cells of Hydrogen obacter thermophilus. The gene was shown to contain a signal sequence. As a next step, the gene without the signal sequence was prepared. When Escherichia coli was transformed with the plasmid containing this gene and was cultivated under nitrate respiration, it produced cytochrome c__-_<552> within cytoplasm fraction.
Cytochrome c__-_<551> gene was also cloned from the total DNA of the cells of Pseudomonas aeruginosa. When Pseudomonas aeruginosa was transformed with the plasmid containig this gene, it produced cytochrome c__-_<551> within periplasm fraction. The production rate was as high as 0.7 % of the total protein of the cell-free extract. An attempt was made to produce cytochrome c__-_<551> by using the other microorganisms (Escherichia coli or Pseudomonas putida), however, successful production could not be obtained.
Reactivity of the two proteins (cytochrome c__-_<552> and cytochrome c__-_<551>) toward electrode was also investigated. Both cytochromes were shown to react with Pt-electrode in a solution state. Cytochrome c__-_<552> protein could react with Au-electrode in the presence of promotor, however, in its absence reactivity was not observed.
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Report
(3 results)
Research Products
(3 results)
