| Project/Area Number |
03556016
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
製造化学・食品
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| Research Institution | Okayama University |
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Principal Investigator |
KAWAZU Kazuyoshi Okayama Univ., Fac. Agr. Prof, 農学部, 教授 (30026520)
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| Co-Investigator(Kenkyū-buntansha) |
KANZAKI Hiroshi Okayama Univ. The graduate School Of Natural Science and Technology Assistant, 大学院・自然科学研究科, 助手 (60183787)
KOBAYASHI Akio Okayama Univ. Agr. Prof., 農学部, 教授 (30115844)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 1992: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1991: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | Nematicide / Bursaphelenchus xylophilus / Paired-ion chromatography / Dehydrosinefungin / nucleoside antibiotic / trunk injection |
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| Research Abstract |
Dehydrosinefungin is the potent nematicidal compound, but the productivity of this compound by Strepromyces sp. H-114 is very low. Thus, an improvement of the productivity is needed. We developed the quantitative method for dehydrosinefungin to find the best conditions for production of this compound. Paired-ion chromatography using an HPLC aparatus resulted in
the best separation of dehydrosinefungin and its analogs, sinefungin and amidinosinefungin. Guided by the productivity of dehydrosinefungin, we determined the best culture conditions. Using the best conditions, the productivity reached more than 20 mg/l, that is, ten times as high as that of original conditions. Large scale cultivation using a jar-fermentor gave us more than 200 mg of pure dehydrosinefugin. This enabled us to carry out the assay using three-year pine trees.
On the other hand, we established the method for a trunk injection of the active compound using morantel tartarate as a test material. Using this method, dehydrosinefungin was injected to three-year pine trees before the treatment of nematodes. We found that 0.5 mg dehydrosinefungin per a three-year pine tree resulted in the resistance of tested trees against wilting. This result indicated that dehydrosinefungin was the promising agent for a pine-wilt desease.
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