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Molecular Immunological Studies on The Prevention and Diagnoses of Protozoan Infection

Research Project

Project/Area Number 03556041
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionOBIHIRO UNIVERSITY, RES.CENTER FOR PROTOZOAN MOLECULAR IMMUNOLOGY

Principal Investigator

SUZUKI Naoyoshi  Prof., Res.Cent.Protozool., Obihiro University, 原虫免疫研, 教授 (10003071)

Co-Investigator(Kenkyū-buntansha) 三浦 弘之  帯広畜産大, 教授 (90003079)
HIROSE Tsuneo  Prof., Vet.Radiology, Obihiro Univ., 獣医放射線学, 教授 (60003076)
OMATA Yoshitaka  Assoc.Prof., Vet.Physiol., Obihiro Univ., 獣医生理学, 助教授 (10132987)
IGARASHI Ikuo  Assoc.Prof., Res.Cent.Protozool., Obihiro Univ., 原虫免疫研, 助教授 (80159582)
SAITO Atushi  Prof., Vet.Physiol., Obihiro University, 獣医生理学, 教授 (10002263)
佐藤 基佳  帯広畜大, 獣医放射線学, 助教授 (50003140)
品川 森一  帯広畜産大学, 畜産学部・獣医公衆衛生学講座, 教授 (00001537)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥16,600,000 (Direct Cost: ¥16,600,000)
Fiscal Year 1993: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1992: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1991: ¥7,800,000 (Direct Cost: ¥7,800,000)
KeywordsImmunoregulator / Lymphokines / Obiopeptides / Toxoplasma / TLA / Immunodeficiency / Blood Cell Stimulating Factor / Physio-Active substance / 免疫調査物質 / リンホカイン / 免疫抑制剤 / サイトカイン / 免疫賦活物質 / 合成ペプチド
Research Abstract

Using newly synthesized peptides on the activity units which were isolated from lymphokines as an immunoregulator, Obiopeptide, the effect of immunosuppression caused by cyclophosphamide to animals cronically infected with Toxoplasma gondii was examined. Mice chronically infected with Toxoplasma, were treated with cyclophosphamide or anti-CD4 monoclonal antibody to identify the effect of these immunosuppressive reagents on the cysts in the brain of infected mice. The effect of obiopeptide as an immunomodulator treatment of the immunosuppressed mice was also analyzed. In the brain of non-treated and cyclophosphamide-treated, chronically Toxoplasma infected mice mainly typicallarge tissue cysts, and sometimes divided cysts, were detected after staining with anti-Toxoplasma ABC technique. In contrast, the brain from anti-CD4 treated, chronically infected mice, contained multiple deeneated Toxoplasma tissue cysts of different size in some partialregions in the brain. Mice chronicaly infect … More ed with Toxoplasma and treated with a combination of cyclophosphamide and obiopeptide showed a significantly higher survival rate than those treated with cyclophosphamide alone. The percentage of neutrophils and lymphocytes in mice treated with a combination of Obiopeptide and anti-CD4, or obiopeptide and cyclophosphamide, was higher than that of mice treated with anti-CD4 or cyclophosphamide alone. These results indicate that reactivaion or rupture of tissue cysts in mice treated with cyclophosphamide, chronically infected with Toxoplasma, might be mainly mediated by CD4 positive cells rather than other immunocomponent cells. The increase of neutrophilic leukocytes might contribute tothe induction of the resistance to Toxoplasma gondii in mice, after treatment with obiopeptide and cyclophosphamide in combination.
On the other hand, an immunopotentiator such as TLA (Toxoplasma Lysate Antigens) was tested in our experimental studies. Growth of the tumor autoinduced by 20-methylcholantherne (MC) in rats was inhibited after administration of TLA.The antitumor activity of TLA was most obvious in the early stage of tumoral growth. When TLA was administered to rats before the appearance of tumor, tumor formation was delayd slightly. According to the immunohistological examination of tumor tissue with anti-Thy-a antibody, the rats treated with TLA showed large Thy-1 positive or Lymphokine-Activated Killer (LAK) cells, whereas the untreated rats indicated only a few small Thy-a positive or NK cells. These observation indicate that TLA is a useful modifier of biological responses to MC-induced tumors. In a series of these studies, TLA induces NK cells, cytotoxic T-lymphocytes and LAK cells in animals after injection, however, the induction of these killer cells needs to combine with monocyte-macrophages in in vivo. Less

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Saito,A.,et.al.: "Effects of reactive oxygen intermediate scavengers on antitoxoplasmic activity of activated macrophages" Parasitology Research. 78. 28-31 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujii,Y.,et.al.: "Restorative effects of a newly synthesized peptide in cyclophosphamide or carragenan pretreated mice infected with opportunistic bacteria" Journal of Protozoology Research. 2. 74-83 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Omata,Y.,et.al.: "Toxoplasma gondii:antibody independent binding of human complement C1q to the parasite" Journal of Protozoology Research. 2. 141-148 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Itou,M.,et.al.: "Studies related to immunosuppression in mice with chronic toxoplasmosis" Journal of Protozoology Research. 3. 99-109 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takahashi,M.,et.al.: "Protective immune response of Isospora felisinfected mice against Babesiamicroti infection" Journal of Vet.Medical Science. 55. 587-590 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Omata,Y.et.al.: "Correlation between antibody levels in T.gondii infected pigs and pathogenicity of the isolated parasites" Veterinary Parasitology. (In press). (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 五十嵐郁男、鈴木直義: ":トキソプラズマ症の感染と免疫" Modern Media(モダンメヂイア), 10 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 鈴木直義: "免疫の仕組み-人・動物共通原虫病から" 大学保健管理業務職員研修会, 7 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Omata, Y., Oikawa, H., Kanda, M., Mikazuki, K., Claveria, F.G., Takehara, T., Saito, A.& Suzuki, N.: "Humoral immune response to Isospora felis and Toxoplasma gondii in cats experimentally infected with Isospora felis" J.Vet.Med.Sci.53. 163-165 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsuki, N., Tamura, S., Ono, K., Watari, T., Goitsuka, R., Yamanobe, A., Hiraga, A., Kubo, K., Takagi, S., Hasegawa, A.& Suzuki, N.: "Excercise-induced phospholipid degradation in the equine skeletal muscle and erythrocytes" J.Vet.Med.Sci.53. 1001-1007 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Omata, Y., Oikawa, H., Kanda, M., Mikazuki, K., Takehara, T., Venturini, C., Saito, A.& Suzuki, N.: "Enhancement of humoral immune response of Isospora felis-infected cats after inoculation with Toxoplasma ghondii" J.Vet.Med.Sci.53. 1071-1073 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shimada, T., Igarasih, I., Maki, Y., Claveria, F.G., Saito, A.& Suzuki, N.: "Cellular subsets involved in protective immunity to Babesia rodhaini infection in BALB/c mice" J.Protozool.Res.1. 35-44 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Miyahara, K., Honda, U., Tose, S., Sakurai, H., Igarashi, I., Saito, A., Hirose, T.& Suzuki, N.: "Therapeutic effects of TLA on 20-methylcholanthrene induced BALB/c mouse tumors" J.Vet.Med.Sci.54. 7-12 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Miyahara, K., Matsumoto, T., Tose, S., Sakurai, H., Igarashi, I., Saito, A., Hirose, T.& Suzuki, N.: "Characteristics of cytotoxic cells induced by Toxoplasma lysate antigen (TLA) in mouse spleen" J.Vet.Med.Sci.54. 87-93 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Miyahara, K., Yokoo, N., Sakurai, H., Igarashi, I., Sakata, Y., Yoshida, Y., Saito, A., Hirose, T.& Suzuki, N.: "Antitumor activity of TLA in methycholanthrene induced tumor-bearing rats" J.Vet.Med.Sci.54. 221-228 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujii, Y., Maki, Y., Claveria, F.G., Igarashi, I., Saito, A., Ono, K.& Suzuki, N.: "Effects of Obiopeptide and Obioactin on the toxoplasmacidal activity, glucose consumption and rufle formation in mouse macrophages" J.Vet.Med.Sci.54. 351-353 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kono, M., Fujii, Y., Shikano, S., Ono, K.& Suzuki, N.: "Influence of a newly synthesized peptide, Obiopeptide-1, as a biological response modifier, upon the lysosomal enzyme activities and chemotactic responses of mouse macrophages" J.Vet.Med.Sci.54. 793-795 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shimada, T., Shikano, S., Ono, K., Saito, A.& Suzuki, N.: "Changes of splenic lymphocyte subpopulation in mice inoculated with Babesia microti and Babesia rodhaini" J.Vet.Med.Sci.54. 1071-1075 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Saito, A., Igarashi, I., Miyahara, K., Ventrurini, C., Claveria, F.G., Hirose, T., Suzuki, N.& Ono, K.: "Effects of reactive oxygen intermediate scavengers on antitoxoplasmic activity of activated macrophages" Parasitol.Res.78. 28-31 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujii, Y., Maki, Y., Ito, M., Saito, A., Igarashi, I., Ono, K., Itoh, K., & Suzuki, N.: "Restorative effects of a newly synthesized peptide, Obiopeptide-1, in cyclophosphamide or carragenan-pretreated mice infected with opportunistic bacteria" J.Protozool.Res.2. 74-83

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Claveria, F.G., Omata, Y., Takehara, T., Saito, A., Maki, Y., Igarashi, I., & Suzuki, N.: "Anti-Babesia rodhaini monoclonal antibodies : effect against Babesia rodhaini and cross-reactivity with human strain of Babesia microti" J.Protozool.Res.2. 112-122 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Itou,M.,et.al.: "Studies related to immunosuppression in mice with chronic toxoplasmosis" Journal of Protozoology Research. 3. 99-109 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takahashi,M.,et.al.: "Protective immune response of Isospora felisinfected mice against Babesiamicroti infection" Journal of Vet.Medical Science. 55. 587-590 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Omata,Y.et.al.: "Correlation between antibody levels in T.gondii infected pigs and pathogenicity of the isolated parasites" Veterinary Parasitology. (In press). (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 五十嵐郁男,鈴木直義: ":トキソプラズマ症の感染と免疫" Modern Meadia(モダンメヂイア), 10 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 鈴木直義: "免疫の仕組み一人・動物共通原虫病から" 大学保健管理業務職員研修会, 7 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shimada,T.,et al: "Changes of splenic lymphocyte subpopulation in mice inoculated with B.microti and B.rodhaini" J.Vet.Med.Sci.54. 1071-1075 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Claveria,F.C.et al: "Anti-Babesia rodhaini nonoclonal antibodies" J.Protozool.Res.2. 112-122 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Fujii,Y.et al: "Restorative effects of a newly synthesized peptide,Obiopeptide-1,in cyclophpsphamide or carrageenan pretreated mice" J.Protozool.Res.2. 74-83 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Saito,A.,et al: "Effects of reactive oxygen intermediate scavengers on antitoxoplasmic activety of activated macrophages" Parasitol.Res. 78. 28-31 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Miyahara,K.,et al: "Antirumor activity of TLA in methylcholanthrer-induced tumor-bearing rats" J.Vet.Med Sci.54. 221-228 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Miyahara,K.,et al: "Characteristics of cytotoxic cells induced by TLA in mouse spleen" J.Vet.Med.Sce.54. 87-93 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Suzuki,Naoyoshi et al: "In vitro cytocidal effect of novel synthetic obiopeptide 1,2 and 3 on Toxoplasma gondii" Allergy & Immunology. 9. 149-158 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sakurai,Haruhisa et al: "In vitro and in vivo studies of the growth inhibitory effect of a newly synthesized peptide,Obiopeptide-1,on mice-bearing methylcholanthrene induced tumour" Journal of Veterinary Medical Science. 53. 823-831 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Fujii,Yasuhiro: "Effect of obiopeptide and obioactin on the toxoplasmacidal activity,glucose consumption and ruffle formation" Journal of Veterinary Medical Science. 54. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Fujii,Yasuhiro: "Restorative effects of an immunoregulator,obiopeptide,in cyclophosphamide treated mice with bacteria infection" Journal of Protozoology Research. 2. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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