| Project/Area Number |
03557011
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Kobe University School of Medicine |
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Principal Investigator |
TANAKA Chikako Kobe Univ. Sch. Med., Dept Pharmacol., Professor, 医学部, 教授 (20025571)
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| Co-Investigator(Kenkyū-buntansha) |
KASE Hiroshi Kyowa Hakko Kogyo Co. Ltd., Pharmaceuticals Res., Chief Researcher, 医薬研究所, 主任研究員
SHUNTOH Hisato Kobe Univ. Sch. Allied Med., Dept. Occupational Therapy. Associate Professor, 医療短大部, 助教授 (70206259)
SAITO Naoaki Kobe Univ. Sch. Med., Dept. Pharmacol., Assistant Professor, 医学部, 講師 (60178499)
KUNO Takayoshi Kobe Univ. Sch. Med., Dept. Pharmacol., Associate Professor, 医学部, 助教授 (50144564)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥18,100,000 (Direct Cost: ¥18,100,000)
Fiscal Year 1992: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1991: ¥16,300,000 (Direct Cost: ¥16,300,000)
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| Keywords | Protein Kinase / Protein Kinase C / Protein Kinase A / GABA_B receptor / Calcineurin / Expression / Subtype / Protein phosphatase / 蛋白質燐酸化反応 / 脱燐酸化反応 / 細胞内情報伝達 / カルシニュ-リン / cDNAクロ-ニング / プロテインキナ-ゼC / 大量発現 |
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| Research Abstract |
Protein Kinases and protein phosphatases have been thought to play pivotal roles in cellular signal transduction. Recent advances in molecular biological techniques have revealed that many of these enzymes have several subtypes coded by distinct genes. In this study, we aimed to establish cell lines which specifically express a subtype of these enzymes to clarify the physiological role of these subtypes. Obtained results are as follows: 1) Two subtypes of the catalytic subunit of calcineurin (alpha and beta) were expressed in E. coli and specific antibodies were prepared by injecting them to rabbit. Distribution of these two subtypes in the rat brain were studied immunohistochemically using these specific antibodies. Effects of FK506/FKBP complex on the activity of calcineurin containing these subtypes were also studied using immunoprecipitated samples by these antibodies. 2) We cloned a cDNA which encodes a novel testis-specific subtype of the regulatory subunit of calcineurin, providing the evidence that there are at least two distinct genes coding the regulatory subunit of calcineurin. 3) We cloned two subtypes of the catalytic subunit of protein kinase A (alpha and beta), and their distribution in various rat tissues and various cell lines were studied. 4) Distribution of various subtypes of protein kinase C in nigro-striatal pathway was studied. 5) Change in the expression of protein kinase C subtypes during development of nervous tissue was studied and it is suggested that ontogeny of each subtype is different. 6) GABAB receptor and protein kinase C were expressed in Xenopus oocyte and it is shown that the function of GABA_B receptor is regulated by protein kinase C.
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