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Production of the two enzymes concerned in peptide C-terminal alpha-amidation by genetic engineering and in vitro reconstitution of peptide C-terminal alpha-amidation reaction

Research Project

Project/Area Number 03557016
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

OKAMOTO Hiroshi  Tohoku University, School of Medicine, Department of Biochemistry, Professor, 医学部, 教授 (60025632)

Co-Investigator(Kenkyū-buntansha) NATA Koji  Tohoku University, School of Medicine, Department of Biochemistry, Research Asso, 医学部, 助手 (90202233)
YONEKURA Hideto  Tohoku University, School of Medicine, Department of Biochemistry, Lecturer, 医学部, 講師 (80240373)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥16,200,000 (Direct Cost: ¥16,200,000)
Fiscal Year 1993: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1992: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1991: ¥7,200,000 (Direct Cost: ¥7,200,000)
KeywordsC-terminal alpha-amidation / Two-step mechanism / Reconstitution / Peptidylglycine hydroxylase / Monooxygenase / Peptidyamidoglycolate lyase / Genetic engineering / Vasoactive Intestinal Peptide / 水酸化グリシン脱アルキルアミド化酵素 / PHM-27 / ペプチドC末端アミド化酵素 / ヒスチジン / 部位特異的変異法 / 発現ベクター / 発現ベクタ- / cDNA / トランスフェクション / 分泌
Research Abstract

Many peptide hormones and neurotransmitters have the C-terminal alpha-amide structure and these alpha-amidated peptides are enzymatically synthesized from their glycine-extended precursors. The C-terminal alpha-amidation reaction proceeds via a two step reaction through a peptidyl alpha-hydroxylglycine as an intermediate and these steps of the peptide alpha-amidation reaction are catalyzed by two distinct enzymes (peptidylglycine hydroxylase and peptidylamidoglycolate lyase). The aim of this project was to produce the two enzymes concerned in peptide alpha-amidation by genetic engineering and reconstitute the peptide alpha-amidation system in vitro.
In this project,
(1) By functional expression of rat "peptidylglycine alpha-amidating enzyme" cDNA, we found that peptidylglycine hydroxylase and peptidylamidoglycolate lyase are synthesized from a single mRNA.
(2) We found that peptidylglycine hydroxylase and peptidylamidoglycolate lyase are encoded in the 5'-half and 3'-half of the mRNA, res … More pectively.
(3) We constructed expression vectors carrying the 5'-half or 3'-half of the cDNA, which directed peptidylglycine hydroxylase or peptidylamidoglycolate lyase to secrete into the culture medium, and produced the two enzymes by transfection of the expression vectors into COS-7 cells.
(4) By experiments using ^<18>O_2, we demonstrated that the peptidylglycine alpha-hydroxylation is a monooxygenase reaction.
(5) By site-directed mutagenesis, we demonstrated that the five histidine residues at position 107, 108, 172, 242 and 244 are essential for the peptidylglycine hydroxylase activity.
(6) We found that the peptidylamidoglycolate lyase exhibited a pH optimum at 5.5 and required a divalent cation but neither O_2 nor ascorbate for the enzyme activity.
(7) We demonstrated that, in the combined presence of peptidylglycine hydroxylase and peptidylamidoglycolate lyase, the mature alpha-amidated peptide was successfully produced from the glycine-extended precursor in vitro.
(8) We produced transgenic mice carrying human Vosoactive Intestinal Peptide (VIP)/PHM-27 gene under the control of insulin promoter and found that VIP and PHM-27 produced from the transgenic pancreatic beta-cells efficiently enhanced the glucose-induced insulin secretion, indicating that VIP and PHM-27 from the transgene were efficiently alpha-amidated by peptidylglycine hydroxylase and peptidylamidoglycolate lyase in the beta-cell. Less

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (234 results)

All Other

All Publications (234 results)

  • [Publications] Okamoto,H.: "Peptide α-amidation is achieved by two distinct enzyme molecules encoded in a single mRNA" Proceeding of the Yamada Conference. 27. 113-116 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kitagawa,M.: "rig encodes ribosomal protein S15.:The primary structure of mammalian ribosomal protein S15." FEBS Letters. 283. 210-214 (1991)

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      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sugawara,A.: "Sequence of the chicken rig gene encoding ribosomal protein S15." Gene. 108. 313-314 (1991)

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    • Related Report
      1993 Final Research Report Summary
  • [Publications] Okamoto,S.: "A diurnal variation of vasoactive intestinal peptide(VIP) mRNA under a daily light-dark cycle in the rat suprachiasmatic nucleus." Histochemistry. 95. 525-528 (1991)

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      1993 Final Research Report Summary
  • [Publications] Hasegawa,S.: "Isolation of a cDNA encoding chicken insulin precursor." Anim.Sci.Technol.(Jpn.). 62. 867-869 (1991)

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      1993 Final Research Report Summary
  • [Publications] Noguchi,M.: "The sorce of oxygen atom in the α-hydroxyglycine intermediate of peptidylglycin α-amidating reaction." Proceeding of the first biennial international conference on amino acid research frontiers and new horizons.61 (1991)

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      「研究成果報告書概要(和文)」より
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      1993 Final Research Report Summary
  • [Publications] 加藤一郎: "ペプチドC末端アミド化反応を触媒する2つの酵素は一本のmRNAより合成される." 生化学. 63. 1209-1213 (1991)

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      1993 Final Research Report Summary
  • [Publications] Okamoto,H.: "The reaction mechanism and the enzymes concerned in the carboxyl-terminal α-amidation of VIP and related peptides" Biomedical Research,Suppl.1. 12. 3 (1991)

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      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 那谷耕司: "膵ホルモン前駆体の構造と進化." 分子糖尿病学. 2. 27-32 (1991)

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      1993 Final Research Report Summary
  • [Publications] 米倉秀人: "reg遺伝子の構造と組織特異的発現." 日本臨床,増刊号. 49. 752-759 (1991)

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      「研究成果報告書概要(和文)」より
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      1993 Final Research Report Summary
  • [Publications] 志賀清人: "rig遺伝子の構造と発現" 日本臨床,増刊号. 49. 760-768 (1991)

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      1993 Final Research Report Summary
  • [Publications] 高沢伸: "rig(rat insulinoma gene)のコードする細胞内蛋白の同定." 糖尿病,Supplement. 34. 159 (1991)

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      「研究成果報告書概要(和文)」より
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      1993 Final Research Report Summary
  • [Publications] 渡辺琢夫: "胎生期および生後ラット膵におけるregの発現." 糖尿病,Supplement. 34. 161 (1991)

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      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 那谷耕司: "ニワトリPancreatic Polypeptide cDNA及び遺伝子の構造決定;Pancreatic Polypeptide遺伝子のモザイク進化." 糖尿病,Supplement. 34. 174 (1991)

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      1993 Final Research Report Summary
  • [Publications] 飯田年以: "ペプチドC末端アミド化反応に関与する脱アルキル化酵素の性質." 生化学. 63. 759 (1991)

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      「研究成果報告書概要(和文)」より
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      1993 Final Research Report Summary
  • [Publications] 野口正人: "ペプチドホルモンのC末端アミド化反応においてα-ヒドロキシグリシン中間体にとりこまれる酸素原子の由来." 生化学. 63. 761 (1991)

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      1993 Final Research Report Summary
  • [Publications] 高沢伸: "酵母rig遺伝子の構造決定とその産物の同定." 生化学. 63. 847 (1991)

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      1993 Final Research Report Summary
  • [Publications] 志賀清人: "ヒトrig遺伝子の転写調節因子." 生化学. 63. 900 (1991)

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      1993 Final Research Report Summary
  • [Publications] 海野倫明: "2種のマウスreg遺伝子(reg-I,reg-II)の構造と発現." 生化学. 64. 1012 (1991)

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      1993 Final Research Report Summary
  • [Publications] 那谷耕司: "Pancreatic Polypeptide遺伝子の分子進化." 生化学. 64. 1012 (1991)

    • Description
      「研究成果報告書概要(和文)」より
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      1993 Final Research Report Summary
  • [Publications] 宮崎修吉: "食道癌における原癌遺伝子c-myc発現の多角的解析." Proceedings of the Japanese Cancer Association(Jpn.J.Cancer Res.). 115 (1991)

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  • [Publications] 木村伯子: "Human reg mRNAの局在とその蛋白の膵腫瘍診断における有用性." Proceedings of the Japanese Cancer Association(Jpn.J.Cancer Res.). 309 (1991)

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      1993 Final Research Report Summary
  • [Publications] 日高浩史: "Pancreatic Polypeptide遺伝子のモザイク進化;ニワトリPancreatic Polypeptide cDNAおよび遺伝子の構造決定." 生化学. 63. 1259 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 志賀清人: "ヒトrig遺伝子の転写調節領域と転写因子." 生化学. 63. 1259 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Noguchi,M.: "The source of oxygen atom in the α-hydroxyglycine intermediate of the peptidylglycine α-amidating reaction." Biochemical Journal. 283. 883-888 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Okamoto,H.: "Cyclic ADP-ribose,a novel second messenger for intracellular Ca^<++> mobilization in pancreatic islets." 1st IUBMB Conference Abstracts. 218 (1992)

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      1993 Final Research Report Summary
  • [Publications] Takasawa,S.: "Structural determination of Saccharomyces cerevisiae rig gene and identification of its product as ribosomal protein S21." FEBS Letters. 307. 318-323 (1992)

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      1993 Final Research Report Summary
  • [Publications] Takasawa,S.: "The primary structure of rat rig/ribosomal protein S15 gene." Biochimica et Biophysica Acta. 1132. 228-230 (1992)

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      1993 Final Research Report Summary
  • [Publications] Kimura,N.: "Detection of multiple hormones and their mRNAs in human neuroblastoma cell line NB-1 using in situ hybridization,immunocytochemistry and radioimmunoassay." Virchows Archiv.B Cell Pathol.62. 321-327 (1992)

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      1993 Final Research Report Summary
  • [Publications] Kimura,N.: "Expression of reg mRNA and its product in human normal and neoplastic pancreas." Cancer. 70. 1857-1863 (1992)

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      1993 Final Research Report Summary
  • [Publications] 海野倫明: "The reg gene family(regIとregII)の構造と発現." 分子糖尿病学. 3. 19-25 (1992)

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      1993 Final Research Report Summary
  • [Publications] 海野倫明: "膵ラ島再生蛋白(reg蛋白)とPancreatic stone protein." 最新医学. 47. 2096-2097 (1992)

    • Description
      「研究成果報告書概要(和文)」より
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      1993 Final Research Report Summary
  • [Publications] 海野倫明: "reg遺伝子ファミリーの構造と発現の組織特異性." 糖尿病,Supplement. 35. 138 (1992)

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      1993 Final Research Report Summary
  • [Publications] 那谷耕司: "Pancreatic Polypeptide family遺伝子の分子進化:ニワトリNeuropeptide Y cDNAの構造決定." 糖尿病,Supplement. 35. 143 (1992)

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      1993 Final Research Report Summary
  • [Publications] 安斎高穂: "変異体を用いたペプチドホルモンC末端アミド化酵素活性中心の解析." 生化学. 64. 1162 (1992)

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      1993 Final Research Report Summary
  • [Publications] 東郷暁: "ラットInitiation Factor3(IF3) γ subunitの精製と構造決定." 生化学. 64. 1163 (1992)

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      1993 Final Research Report Summary
  • [Publications] 海野倫明: "マウス膵・胆嚢におけるreg遺伝子ファミリーの発現." 膵臓-日本膵臓学会誌(J.Jpn.Panc.Soc.). 7. 395 (1992)

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  • [Publications] 鈴木由久: "reg遺伝子ファミリーに属する新しい遺伝子の分離とその発現." 生化学. 64. 681 (1992)

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      1993 Final Research Report Summary
  • [Publications] 飯田年以: "ペプチドC末端アミド化反応に関与するリアーゼの構造と機能." 生化学. 64. 715 (1992)

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      1993 Final Research Report Summary
  • [Publications] 東郷暁: "いわゆる“68kDaラミニン結合蛋白質のcDNA"の実体について." 生化学. 64. 746 (1992)

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      1993 Final Research Report Summary
  • [Publications] 安斎高穂: "ヒト神経芽細胞腫NB-1におけるペプチドホルモンC末端アミド化酵素遺伝子の発現誘導" 生化学. 64. 865 (1992)

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      1993 Final Research Report Summary
  • [Publications] 米倉秀人: "ペプチドホルモンC末端アミド化酵素活性中心.-部位特異的変異法を用いた解析-" 生化学. 64. 866 (1992)

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  • [Publications] Takasawa,S.: "Synthesis and hydrolysis of cyclic ADP-ribose by human leukocyte antigen CD38 and inhibition of the hydrolysis by ATP." Journal of Biological Chemistry. 268. 26052-26054 (1993)

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  • [Publications] Wulff,B.S.: "Efficient amidation of C-peptide deleted NPY precursors by non-endocrine cells is affected by the presence of Lys-Arg at the C-terminus." Molecular and Cellular Endocrinology. 91. 135-141 (1993)

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  • [Publications] Nata,K.: "Structure determination and evolution of the chicken cDNA and gene encoding prepropancreatic polypeptide" Gene. 130. 183-189 (1993)

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  • [Publications] Miyazaki,S.: "Analysis of c-myc oncogene in human esophageal carcinoma:immunohistochemistry,in situ hybridization and northern and Southern blot studies." Anticancer Reseach. 12. 1745-1755 (1992)

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  • [Publications] Kimura,N.: "Multiple-hormone gene expression in ganglioneuroblastoma with watery diarrhea,hypokalemia and achlorhydria syndrome." Cancer. 71. 2841-2846 (1993)

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  • [Publications] Sasaki,T.: "Cyclic ADP-ribose,a candidate for a novel Ca^<2+>-mobilizing second messenger,induced Ca^<2+>-dependent current responses in airway submucosal gland cells." American Review of Respiratory Disease. 147. A936 (1993)

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  • [Publications] 郡和宏: "ヒトPeptide YY前駆体cDNAの単離と構造決定." 生化学. 64. 909 (1992)

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  • [Publications] 高沢伸: "cyclic ADP-ribose:インスリン産生細胞の新しい細胞内情報伝達物質." 生化学. 64. 921 (1992)

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      1993 Final Research Report Summary
  • [Publications] 那谷耕司: "cyclic ADP-ribose合成酵素(ADP-ribosyl cyclase)の精製." 生化学. 64. 921 (1992)

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  • [Publications] Takasawa,S.: "Cyclic ADP-ribose in insulin secretion from pancreatic β cells." Science. 259. 370-373 (1993)

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  • [Publications] Unno,M.: "Structure,chromosomal localization,and expression of mouse reg genes,reg I and reg II:A novel type of reg gene,reg II,exists in the mouse genome." Journal of Biological Chemistry. 268. 15974-15982 (1993)

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  • [Publications] Takasawa,S.: "Cyclic ADP-ribose in β cells." Science. 262. 584-586 (1993)

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  • [Publications] Kohri,K.: "Cloning and structural determination of human peptide YY cDNA and gene." Biochimica et Biophysica Acta. 1173. 345-349 (1993)

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  • [Publications] Satomura,Y.: "The immunohistochemical evaluation of PSP/reg-protein in normal and diseased human pancreatic tissues." International Journal of Pancreatology. 13. 59-67 (1993)

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  • [Publications] Okamoto,H.: "ADP-ribosyl cyclase that synthesizes cyclic ADP-ribose,a second messenger for Ca2+ mobilization in β-cells." Diabetes. 42. 76A (1993)

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  • [Publications] Okamoto,H.: "B-cell regeneration:the role of the reg gene" 1st International Meeting of the Pancreatic Islet Study group Abstract. 111 (1993)

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  • [Publications] Watanabe,T.: "Pancreatic β-cell replication and amelioration of surgical diabetes by reg protein." Proceedings of the National Academy of Sciences,U.S.A.(in press). (1994)

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  • [Publications] Moriizumi,S.: "Isolation,structural determination and expression of a novel reg gene,human regIβ." Biochimica et Biophysica Acta. 1217. 199-202 (1994)

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  • [Publications] Tohgo,A.: "Structural determination and characterization of a 40 kDa protein isolated from rat 40S ribosomal subunit." FEBS Letters. 340. 133-138 (1994)

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  • [Publications] Suzuki,Y.: "Structure and expression of a novel rat regIII gene." Gene. (in press). (1994)

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  • [Publications] 森泉茂樹: "膵の再生とreg遺伝子ファミリー" 医学の歩み. 別冊. 135-138 (1993)

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      1993 Final Research Report Summary
  • [Publications] 高沢伸: "膵β細胞の新しいセカンドメッセンジャーcyclic ADP-ribose." 分子糖尿病学. 4. 1-6 (1993)

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      1993 Final Research Report Summary
  • [Publications] 高沢伸: "膵β細胞機能発現の新しい細胞内情報伝達物質cyclic ADP-ribose." 糖尿病記録号1993. 247-251 (1994)

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  • [Publications] 加藤一郎: "膵ランゲルハンス島β細胞でVIP遺伝子を過剰発現するトランスジェニックマウスの作製と導入遺伝子による血糖降下作用." 分子糖尿病学. 5(印刷中). (1994)

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  • [Publications] 海野倫明: "ラット膵切除後・実験膵炎時に発現する新しいreg遺伝子について." 日本外科学会誌,総会号. 17. 152 (1993)

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      1993 Final Research Report Summary
  • [Publications] 高沢伸: "膵β細胞機能発現の新しい細胞内情報伝達物質cyclic ADP-ribose." 糖尿病,Supplement. 36. 139 (1993)

    • Description
      「研究成果報告書概要(和文)」より
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      1993 Final Research Report Summary
  • [Publications] 森泉茂樹: "reg遺伝子ファミリーの構造と機能." 糖尿病,Supplement. 36. 160 (1993)

    • Description
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  • [Publications] 那谷耕司: "ヒトPeptide YY cDNAと遺伝子の単離と構造決定." 糖尿病,Supplement. 36. 332 (1993)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] 森泉茂樹: "新しいヒト遺伝子の単離-ヒトreg遺伝子ファミリーの構造・発現・染色体座位." 生化学. 65. 1232 (1993)

    • Description
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  • [Publications] 那谷耕司: "Aplysia ADP-ribosyl cyclaseの遺伝子構造." 生化学. 65. 1232 (1993)

    • Description
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  • [Publications] 森泉茂樹: "reg遺伝子ファミリーに属する新しいヒト遺伝子(Human regI β)の分離と染色体マッピング." 膵臓-日本膵臓学会誌(J.Jpn.Panc.Soc.). 18. 308 (1993)

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  • [Publications] 岡本宏: "Cyclic ADP-riboseをセカンドメッセンジャーとする新しい細胞内情報伝達系." 日本内分泌学会雑誌. 69. 168 (1993)

    • Description
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  • [Publications] 加藤一郎: "ヒトVasoactive Intestinal Peptide(VIP)遺伝子を膵ランゲルハンス島β細胞内で過剰発現するトランスジェニックマウスの作製." 生化学. 65. 812 (1993)

    • Description
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  • [Publications] 渡辺琢夫: "reg蛋白の膵ランゲルハンス島β細胞増殖作用." 生化学. 65. 1082 (1993)

    • Description
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  • [Publications] 宮下曜: "ラットreg遺伝子の構造と発現調節機構." 生化学. 65. 1082 (1993)

    • Description
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  • [Publications] 森泉茂樹: "新しい増殖因子“regファミリー"に属する二つのヒト遺伝子(regIα・regIβ)の構造・発現・染色体座位." 生化学. 65. 1082 (1993)

    • Description
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      1993 Final Research Report Summary
  • [Publications] 那谷耕司: "ADP-ribosyl cyclase cDNA・遺伝子の構造と発現." 生化学. 65. 1088 (1993)

    • Description
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  • [Publications] 高沢伸: "ヒトcyclic ADP-ribose合成系とその調節機構." 生化学. 65. 1088 (1993)

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  • [Publications] Shao-Ying Hua: "Cyclic ADP-ribose modulates Ca^<2+> release channels for activation by physiological Ca^<2+> entry in bullfrog sympathetic neurons." Neuron. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
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  • [Publications] 岡本宏: "膵β細胞破壊と防止についての岡本モデルとその展開." 糖尿病. 37. 99-102 (1994)

    • Description
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  • [Publications] Yonekura,H.: "Identification of the catalytically essential histidine of peptidylglycine monooxygenase." FEBS Letters. (submitted). (1994)

    • Description
      「研究成果報告書概要(和文)」より
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      1993 Final Research Report Summary
  • [Publications] Kato,I.: "Transgenic mice overexpressing human vasoactive intestinal peptide gene in pancreatic β-cells." Journal of Biological Chemistry. (submitted). (1994)

    • Description
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      1993 Final Research Report Summary
  • [Publications] Iida,T.: "Purification and characterization of peptidylglycine monooxygenase and peptidylamidoglycolate lyase from equine serum." Archives of Biochemistry and Biophysics. (submitted). (1994)

    • Description
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  • [Publications] Okamoto,H.: "Mechanisms of destruction of insulin-secreting cells.in Nutrient Regulation of Insulin secretion." Flatt,P.R.,ed.(Portland Press,Colchester,England), 387-406 (1991)

    • Description
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  • [Publications] Okamoto,H.: "Islet B-cell regeneration and reg genes.in Diabetes 1991" H.Rifkin,J.A.Colwell and S.I.Taylor,eds(Excepta Medica,Amsterdam), 268-270 (1991)

    • Description
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  • [Publications] 米倉秀人: "PP(膵ポリ・ペプチド) 新生化学実験講座,第9巻,ホルモン" 日本生化学会編,(東京化学同人), 154-166 (1991)

    • Description
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  • [Publications] Unno,M.: "Islet B-cell regeneration and reg genes.in Pancreatic Islet Cell Regeneration and Growth" Vinik,A.I.,ed.(Plenum Publishing Corp.), 61-66 (1991)

    • Description
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      1993 Final Research Report Summary
  • [Publications] Yonekura,H.: "Structue and expression of reg gene family.in Frontiers of Insulin Secretion and Pancreatic B-Cell Research" Flatt,P.R.,and Lenzan S.,eds(in press), (1994)

    • Description
      「研究成果報告書概要(和文)」より
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      1993 Final Research Report Summary
  • [Publications] Takasawa,S.: "A cyclic ADP-ribose,a NAD+ metabolite,in stimulus-secretion coupling in pancreatic B-cells.in Frontiers of Insulin Secretion and Pancreatic B-Cell Research" Flatt,P.R.,and Lenzan S.,eds(in press), (1994)

    • Description
      「研究成果報告書概要(和文)」より
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      1993 Final Research Report Summary
  • [Publications] Okamoto, H., Kato, I., Noguchi, M., Takahashi, K., Yonekura, H., Anzai, T., Yamamoto, H., Tajima, M., Iida, T.and Yanagi, M.: "Peptide alpha-amidation is achieved by two distinct enzyme molecules encoded in a single mRNA." Proceeding of the Yamada Conference XXVII. 113-116 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] Kato, I., Yonekura, H.and Okamoto, H.: "Two enzymes concerned in peptide hormone alpha-amidation are synthesized from a single mRNA." Seikagaku. 63. 1209-1213 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
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      1993 Final Research Report Summary
  • [Publications] Okamoto, H.: "Mechanisms of destruction of insulin-secreting cells." Nutrient Regulation of Insulin Secretion(Flatt, P.R., ed.), Portland Press, Colchester, England. 387-406 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Okamoto, H.: "Islet B-cell regeneration and reg genes." Diabetes 1991(H.Rifkin, J.A.Colwell and S.I.Taylor, eds) Excepta Medica, Amsterdam. 268-270 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
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      1993 Final Research Report Summary
  • [Publications] Kitagawa, M., Takasawa, S., Kikuchi, N., Itoh, T., Teraoka, H., Yamamoto, H.and Okamoto, H.: "rig encodes ribosomal protein S15. : The primary structure of mammalian ribosomal protein S15." FEBS Lett.283. 210-214 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
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      1993 Final Research Report Summary
  • [Publications] Sugawara, A., Shiga, K., Takasawa, S., Yonekura, H., Yamamoto, H.and Okamoto, H.: "Sequence of the chicken rig gene encoding ribosomal protein S15." Gene. 108. 313-314 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
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      1993 Final Research Report Summary
  • [Publications] Okamoto, S., Okamura, H., Miyake, M., Takahashi, Y., Takagi, S., Akagi, Y., Fukui, K., Okamoto, H.and Ibata, Y.: "A diurnal variation of vasoactive intestinal peptide(VIP) mRNA under a daily light-dark cycle in the rat suprachiasmatic nucleus." Histochemistry. 95. 525-528 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
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      1993 Final Research Report Summary
  • [Publications] Hasegawa, S., Honda, K., Nata, K., Yonekura, H., Okamoto, H.and Hikami, Y.: "Isolation of a cDNA encoding chicken insulin precursor." Anim.Sci.Technol.(Jpn.). 62. 867-869 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] Noguchi, M., Seino, H., Kochi, H., Okamoto, H., Tanaka, T.and Hirama, T.: "The sorce of oxygen atom in the alpha-hydroxyglycine intermediate of peptidylglycine alpha-amidating reaction." Proceeding of the first biennial international conference on amino acid research frontiers and new horizons. 61 (1991)

    • Description
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  • [Publications] Okamoto, H., Kato, I., Yonekura, H., Anzai, T., Tajima, M., Iida, T.and Yanagi, M.: "The reaction mechanism and the enzymes concerned in the carboxyl-terminal alpha-amidation of VIP and related peptides." Biomedical Research. 12 Suppl.1. 3 (1991)

    • Description
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  • [Publications] Noguchi, M., Seino, H., Kochi, H., Okamoto, H., Tanaka, T.and Hirama, M.: "The source of oxygen atom in the alpha-hydroxyglycine intermediate of the peptidylglycine alpha-amidating reaction." Biochem.J.283. 883-888 (1992)

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  • [Publications] Noguchi, M., Seino, H., Kochi, H., Okamoto, H., Tanaka, T.and Hirama, T.: "The source of oxygen atom in the alpha-hydroxyglycine intermediate of peptidylglycine alpha-amidating reaction." Frontiers and new horizons in amino acid research(Takai, K., ed.) Elsevier Science Publishers B.V., Amsterdam. 511-514 (1992)

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  • [Publications] Okamoto, H., Takasawa, H., Nata, K.and Yonekura, H.: "Cyclic ADP-ribose, a novel second messenger for intracellular Ca^<++> mobilization in pancreatic islets." 1st IUBMB Conference Abstracts. 218 (1992)

    • Description
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      1993 Final Research Report Summary
  • [Publications] Unno, M., Itoh, T., Watanabe, T., Miyashita, H., Moriizumi, S., Teraoka, H., Yonekura, H.and Okamoto, H.: "Islet B-cell regeneration and reg gene." Pancreatic Islet Cell Regeneration and Growth (Vinik, A.I., ed.), Plenum Publishing Corp., New York, London, Washington DC, Boston. 61-66 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] Takasawa, S., Yonekura, H., Tohgo, A., Unno, M., Yamamoto, H.and Okamoto, H.: "Structural determination of Saccharomyces cerevisiae rig gene and identification of its product as ribosomal protein S21." FEBS Lett.307. 318-323 (1992)

    • Description
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  • [Publications] Takasawa, S., Tohgo, A., Unno, M., Shiga, K., Yonekura, H.and Okamoto, H.: "The primary structure of rat rig/ribosomal protein S15 gene." Biochim.Biophys.Acta. 1132. 228-230 (1992)

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  • [Publications] Kimura, N., Yamamoto, H., Okamoto, H., Gotoh, K., Sone, M., Mouri, T., Ota, K., Kimura, T., Ohzeki, T.and Miura, Y.: "Detection of multiple hormones and their mRNAs in human neuroblastoma cell line NB-1 using in situ hybridization, immunocytochemistry and radioimmunoassay." Virchows Archiv.B Cell Pathol.62. 321-327 (1992)

    • Description
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  • [Publications] Kimura, N., Yonekura, H., Nagura, H.and Okamoto, H.: "Expression of reg mRNA and its product in human normal and neoplastic pancreas." Cancer. 70. 1857-1863 (1992)

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  • [Publications] Miyazaki, S., Sasano, H., Shiga, K., Sawai, T., Nishihira, T., Okamoto, H.and Mori, S.: "Analysis of c-myc oncogene in human esophageal carcinoma : immunohistochemistry, in situ hybridization and northern and Southern blot studies." Anticancer Research. 12. 1745-1755 (1992)

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  • [Publications] Takasawa, S., Nata, K., Yonekura, H.and Okamoto, H.: "Cyclic ADP-ribose in insulin secretion from pancreatic beta cells." Science. 259. 370-373 (1993)

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  • [Publications] Wulff, B.S., Catipovic, B., Okamoto, H., Gether, U., Schwartz, T.W.and Johansen, T.E.: "Efficient amidation of C-peptide deleted NPY precursors by non-endocrine cells is affected by the presence of Lys-Arg at the C-terminus." Mol.Cell.Endocrinol.91. 135-141 (1993)

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  • [Publications] Unno, M., Yonekura, H., Nakagawara, K., Watanabe, T., Miyashita, H., Moriizumi, S., Itoh, T., Teraoka, H.and Okamoto, H.: "Structure, chromosomal localization, and expression of mouse reg genes, reg I and reg II : A novel type of reg gene, reg II, exists in the mouse genome." J.Biol.Chem.268. 15974-15982 (1993)

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  • [Publications] Kohri, K., Nata, K., Yonekura, H., Nagai, A., Konno, K.and Okamoto, H.: "Cloning and structural determination of human peptide YY cDNA and gene." Biochim.Biophys.Acta. 1173. 345-349 (1993)

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  • [Publications] Satomura, Y., Sawabu, N., Ohta, H., Watanabe, H., Yamakawa, O., Motoo, Y., Okai, T., Toya, D., Makino, H.and Okamoto, H.: "The immunohistochemical evaluation of PSP/reg-protein in normal and diseased human pancreatic tissues." Int.J.Pancreatol.13. 59-67 (1993)

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  • [Publications] Nata, K., Sugimoto, T., Kohri, K., Hidaka, H., Hattori, E., Yamamoto, H., Yonekura, H.and Okamoto, H.: "Structure determination and evolution of the chicken cDNA and gene encoding prepropancreatic polypeptide." Gene. 130. 183-189 (1993)

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  • [Publications] Takasawa, S., Nata, K., Yonekura, H.and Okamoto, H.: "Cyclic ADP-ribose in beta cells." Science. 262. 584-586 (1993)

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  • [Publications] Takasawa, S., Tohgo, A., Noguchi, N., Nata, K., Sugimoto, T., Yonekura, H.and Okamoto, H.: "Synthesis and hydrolysis of cyclic ADP-ribose by human leukocyte antigen CD38 and inhibition of the hydrolysis by ATP." J.Biol.Chem.263. 26052-26054 (1993)

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  • [Publications] Kimura, N., Yamamoto, H., Okamoto, H., Wakasa, H.and Nagura, H.: "Multiple-hormone gene expression in ganglioneuroblastoma with watery diarrhea, hypokalemia and achlorhydria syndrome." Cancer. 71. 2841-2846 (1993)

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  • [Publications] Sasaki, T., Shimura, S., Takasawa, S., Nagaki, M., Satoh, M., Okamoto, H.and Shirato, K.: "Cyclic ADP-ribose, a candidate for a novel Ca^<2+> -mobilizing second messenger, induced Ca^<2+> -dependent current responses in airway submucosal gland cells." American Review of Respiratory Disease. 147. A936 (1993)

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  • [Publications] Okamoto, H., Takasawa, S., Nata, K., Sugimoto, T., Noguchi, N., Takeyama, J.and Yonekura, H.: "ADP-ribosyl cyclase that synthesizes cyclic ADP-ribose, a second messenger for Ca^<2+> mobilization in beta-cells." Diabetes 42, Supplement 1. 76A. (1993)

    • Description
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  • [Publications] Okamoto, H.: "B-cell regeneration : the role of the reg gene" First International Meeting of the Pancreatic Islet Study Group Abstract(Volume II). 111 (1993)

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  • [Publications] Moriizumi, S., Watanabe, T., Unno, M., Nakagawara, K., Suzuki, Y., Miyashita, H., Yonekura, H.and Okamoto, H.: "Isolation, structural determination and expression of a novel reg gene, human regIbeta." Biochim.Biophys.Acta. 1217. 199-202 (1994)

    • Description
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  • [Publications] Watanabe, T., Yonemura, Y., Yonekura, H., Suzuki, Y., Miyashita, H., Sugiyama, K., Moriizumi, S., Unno, M., Tanaka, O., Kondo, H., Bone, A.J., Takasawa, S.and Okamoto, H.: "Pancreatic beta-cell replication and amelioration of surgical diabetes by reg protein." Proc.Natl.Acad.Sci.U.S.A.(in press). (1994)

    • Description
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  • [Publications] Yonekura, H., Unno, M., Watanabe, T., Moriizumi, S., Suzuki, Y., Miyashita, H., Yonemura, Y., Sugiyama, K.and Okamoto, H.: "Structure and expression of reg gene family." Frontiers of Insulin Secretion and Pancreatic B-Cell Research(Flatt, P.R., and Lenzan S., eds). (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
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      1993 Final Research Report Summary
  • [Publications] Takasawa, S., Nata, K., Yonekura, H.and Okamoto, H.: "A cyclic ADP-ribose, a NAD^+ metabolite, in stimulus-secretion coupling in pancreatic B-cells." Frontiers of Insulin Secretion and Pancreatic B-Cell Research(Flatt, P.R., and Lenzan S., eds). (in press). (1994)

    • Description
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  • [Publications] Tohgo, A., Takasawa, S., Munakata, H., Yonekura, H., Hayashi, N.and Okamoto, H.: "Structural determination and characterization of a 40 kDa protein isolated from rat 40S ribosomal subunit." FEBS Lett.340. 133-138 (1994)

    • Description
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  • [Publications] Suzuki, Y., Yonekura, H., Watanabe, T., Unno, M., Moriizumi, S., Miyashita, H.and Okamoto, H.: "Structure and expression of a novel rat reg III gene." Gene. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
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      1993 Final Research Report Summary
  • [Publications] Yonekura, H., Anzai, T., Kato, I., Furuya, Y., Shizuta, S., and Okamoto, H.: "Identification of the catalytically essential histidine of peptidylglycine Monooxygenase." FEBS Lett.(submitted). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kato, I., Suzuki, Y., Akabane, A., Tanaka, O., Kondo, N., Yoshimoto, T., Yonekura, H.and Okamoto, H.: "Transgenic mice overexpressing human vasoactive intestinal peptide gene in pancreatic beta-cells. EVIDENCE FOR IMPROVED GLUCOSE TOLERANCE AND ENHANCED INSULIN SECRETION BY VIP AND PHM-27 IN VIVO." J.Biol.Chem.(submitted). (1994)

    • Description
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      1993 Final Research Report Summary
  • [Publications] Iida, T., Kamimura, T., Tajima, M., Yanagi, M.and Okamoto, H.: "Purification and characterization of peptidylglycine monooxygenase and peptidylamidoglycolate lyase from equine serum : Interactions between two enzymes concerning to the peptidyl-alpha-amidating reaction." Arch.Biochem.Biophys.(submitted). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Okamoto,H.: "Peptide α-amidation is achieved by two distinct enzyme molecules encoded in a single mRNA" Proceeding of the Yamada Conference. 27. 113-116 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kitagawa,M.: "rig encodes ribosomal protein S15.:The primary structure of mammalian ribosomal protein S15." FEBS Letters. 283. 210-214 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sugawara,A.: "Sequence of the chicken rig gene encoding ribosomal protein S15." Gene. 108. 313-314 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Okamoto,S.: "A diurnal variation of vasoactive intestinal peptide(VIP)mRNA under a daily light-dark cycle in the rat suprachiasmatic nucleus." Histochemistry. 95. 525-528 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hasegawa,S.: "Isolation of a cDNA encoding chicken insulin precursor." Anim.Sci.Technol.(Jpn.). 62. 867-869 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Noguchi,M.: "The sorce of oxygen atom in the α-hydroxyglycine intermediate of peptidylglycin α-amidating reaction." Proceeding of the first biennial international conference on amino acid research frontiers and new horizons.61 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 加藤一郎: "ペプチドC末端アミド化反応を触媒する2つの酵素は一本のmRNAより合成される." 生化学. 63. 1209-1213 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Okamoto,H.: "The reaction mechanism and the enzymes concerned in the carboxyl-terminal α-amidation of VIP and related peptides." Biomedical Research,Suppl.1. 12. 3 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 那谷耕司: "膵ホルモン前駆体の構造と進化." 分子糖尿病学. 2. 27-32 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 米倉秀人: "reg遺伝子の構造と組織特異的発現." 日本臨床,増刊号. 49. 752-759 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 志賀清人: "rig遺伝子の構造と発現" 日本臨床,増刊号. 49. 760-768 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 高沢伸: "rig(rat insulinoma gene)のコードする細胞内蛋白の同定." 糖尿病,Supplement. 34. 159 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 渡辺琢夫: "胎生期および生後ラット膵におけるregの発現." 糖尿病,Supplement. 34. 161 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 那谷耕司: "ニワトリPancreatic Polypeptide cDNA及び遺伝子の構造決定;Pancreatic Polypeptide遺伝子のモザイク進化." 糖尿病,Supplement. 34. 174 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 飯田年以: "ペプチドC末端アミド化反応に関与する脱アルキル化酵素の性質." 生化学. 63. 759 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 野口正人: "ペプチドホルモンのC末端アミド化反応においてα-ヒドロキシグリシン中間体にとりこまれる酸素原子の由来." 生化学. 63. 761 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 高沢伸: "酵母rig遺伝子の構造決定とその産物の同定." 生化学. 63. 847 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 志賀清人: "ヒトrig遺伝子の転写調節因子." 生化学. 63. 900 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 海野倫明: "2種のマウスreg遺伝子(reg-I,reg-II)の構造と発現." 生化学. 64. 1012 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 那谷耕司: "Pancreatic Polypeptide遺伝子の分子進化." 生化学. 64. 1012 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 宮崎修吉: "食道癌における原癌遺伝子c-myc発現の多角的解析." Proceedings of the Japanease Cancer Association(Jpn.J.Cancer Res.). 115 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 木村伯子: "Human reg mRNAの局在とその蛋白の膵腫瘍診断における有用性." Proceedings of the Japanease Cancer Association(Jpn.J.Cancer Res.). 309 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 日高浩史: "Pancreatic Polypeptide遺伝子のモザイク進化;ニワトリPancreatic Polypeptide cDNAおよび遺伝子の構造決定." 生化学. 63. 1259 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 志賀清人: "ヒトrig遺伝子の転写調節領域と転写因子." 生化学. 63. 1259 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Noguchi,M.: "The source of oxygen atom in the α-hydroxyglycine intermediate of the peptidylglycine α-amidating reaction." Biochemical Journal. 283. 883-888 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Okamoto,H.: "Cyclic ADP-ribose,a novel second messenger for intracellular Ca^<++> mobilization in pancreatic islets." 1st IUBMB Conference Abstracts. 218 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takasawa,S.: "Structural determination of Saccharomyces cerevisiae rig gene and identification of its product as ribosomal protein S21." FEBS Letters. 307. 318-323 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takasawa,S.: "The primary structure of rat rig/ribosomal protein S15 gene." Biochimica et Biophysica Acta. 1132. 228-230 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kimura,N.: "Detection of multiple hormones and their mRNAs in human neuroblastoma cell line NB-1 using in situ hybridization,immunocytochemistry and radioimmunoassay." Virchows Archiv.B Cell Pathol.62. 321-327 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kimura,N.: "Expression of reg mRNA and its product in human normal and neoplastic pancreas." Cancer. 70. 1857-1863 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 海野倫明: "The reg gene family(regIとregII)の構造と発現." 分子糖尿病学. 3. 19-25 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 海野倫明: "膵ラ島再生蛋白(reg蛋白)とPancreatic stone protein." 最新医学. 47. 2096-2097 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 海野倫明: "reg遺伝子ファミリーの構造と発現の組織特異性." 糖尿病,Supplement. 35. 138 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 那谷耕司: "Pancreatic Polypeptide family遺伝子の分子進化:ニワトリNeuropeptide Y cDNAの構造決定." 糖尿病,Supplement. 35. 143 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 安斎高穂: "変異体を用いたペプチドホルモンC末端アミド化酵素活性中心の解析." 生化学. 64. 1162 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 東郷暁: "ラットInitiation Factor 3(IF3)γ subunitの精製と構造決定." 生化学. 64. 1163 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 海野倫明: "マウス膵・胆嚢におけるreg遺伝子ファミリーの発現." 膵臓-日本膵臓学会誌(J.Jpn.Panc.Soc.). 7. 395 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 鈴木由久: "reg遺伝子ファミリーに属する新しい遺伝子の分離とその発現." 生化学. 64. 681 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 飯田年以: "ペプチドC末端アミド化反応に関与するリアーゼの構造と機能." 生化学. 64. 715 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 東郷暁: "いわゆる“68kDaラミニン結合蛋白質のcDNA"の実体について." 生化学. 64. 746 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 安斎高穂: "ヒト神経芽細胞種NB-1におけるペプチドホルモンC末端アミド化酵素遺伝子の発現誘導" 生化学. 64. 865 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 米倉秀人: "ペプチドホルモンC末端アミド化酵素活性中心.-部位特異的変異法を用いた解析-" 生化学. 64. 866 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 郡和宏: "ヒトPeptide YY前駆体cDNAの単離と構造決定." 生化学. 64. 909 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 高沢伸: "cyclic ADP-ribose:インスリン産生細胞の新しい細胞内情報伝達物質." 生化学. 64. 921 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 那谷耕司: "cyclic ADP-ribose合成酵素(ADP-ribosyl cyclase)の精製." 生化学. 64. 921 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takasawa,S.: "Cyclic ADP-ribose in insulin secretion from pancreatic β cells." Science. 259. 370-373 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Unno,M.: "Structure,chromosomal localization,and expression of mouse reg genes,regI and regII:A novel type of reg gene,regII,exists in the mouse genome." Journal of Biological Chemistry. 268. 15974-15982 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takasawa,S.: "Cyclic ADP-ribose in β cells." Science. 262. 584-586 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takasawa,S.: "Synthesis and hydrolysis of cyclic ADP-ribose by human leukocyte antigen CD38 and inhibition of the hydrolysis by ATP." Journal of Biological Chemistry. 268. 26052-26054 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Wulff,B.S.: "Efficient amidation of C-peptide deleted NPY precursors by non-endocrine cells is affected by the presence of Lys-Arg at the C-terminus." Molecular and Cellular Endocrinology. 91. 135-141 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nata,K.: "Structure determination and evolution of the chicken cDNA and gene encoding prepropancreatic polypeptide" Gene. 130. 183-189 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Miyazaki,S.: "Analysis of c-myc oncogene in human esophageal carcinoma:immunohistochemistry,in situ hybridization and northern and Southern blot studies." Anticancer Reseach. 12. 1745-1755 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kimura,N.: "Multiple-hormone gene expression in ganglioneuroblastoma with watery diarrhea,hypokalemia and achlorhydria syndrome." Cancer. 71. 2841-2846 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sasaki,T.: "Cyclic ADP-ribose,a candidate for a novel Ca^<2+>-mobilizing second messenger,induced Ca^<2+>-dependent current responses in airway submucosal gland cells." American Review of Respiratory Disease. 147. A936 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kohri,K.: "Cloning and structural determination of human peptide YY cDNA and gene." Biochimica et Biophysica Acta. 1173. 345-349 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Satomura,Y.: "The immunohistochemical evaluation of PSP/reg-protein in normal and diseased human pancreatic tissues." International Journal of Pancreatology. 13. 59-67 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Okamoto,H.: "ADP-ribosyl cyclase that synthesizes cyclic ADP-ribose,a second messenger for Ca2+ mobilization in β-cells." Diabetes. 42. 76A (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Okamoto,H.: "Β-cell regeneration:the role of the reg gene" 1st International Meeting of the Pancreatic Islet Study group Abstract. 111 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Watanabe,T.: "Pancreatic β-cell replication and amelioration of surgical diabetes by reg protein." Proceedings of the National Academy of Sciences,U.S.A.in press. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Moriizumi,S.: "Isolation,structural determination and expression of a novel reg gene,human regIβ." Biochimica et Biophysica Acta. 1217. 199-202 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tohgo,A.: "Structural determination and characterization of a 40 kDa protein isolated from rat 40S ribosomal subunit." FEBS Letters. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Suzuki,Y.: "Structure and expression of a novel rat regIII gene." Gene. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 森泉茂樹: "膵の再生とreg遺伝子ファミリー" 医学の歩み. 別冊. 135-138 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 高沢伸: "膵β細胞の新しいセカンドメッセンジャー cyclic ADP-ribose." 分子糖尿病学. 4. 1-6 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 高沢伸: "膵β細胞機能発現の新しい細胞内情報伝達物質 cyclic ADP-ribose." 糖尿病記録号1993. 247-251 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 加藤一郎: "膵ランゲルハンス島β細胞でVIP遺伝子を過剰発現するトランスジェニックマウスの作製と導入遺伝子による血糖降下作用." 分子糖尿病学. 5(印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 海野倫明: "ラット膵切除後・実験膵炎時に発現する新しいreg遺伝子について." 日本外科学会誌,総会号. 17. 152 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 高沢伸: "膵β細胞機能発現の新しい細胞内情報伝達物質 cyclic ADP-ribose." 糖尿病,Supplement. 36. 139 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 森泉茂樹: "reg遺伝子ファミリーの構造と機能." 糖尿病,Supplement. 36. 160 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 那谷耕司: "ヒトPeptide YY cDNAと遺伝子の単離と構造決定." 糖尿病,Supplement. 36. 332 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 森泉茂樹: "新しいヒト遺伝子の単離-ヒトreg遺伝子ファミリーの構造・発現・染色体座位." 生化学. 65. 1232 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 那谷耕司: "Aplysia ADP-ribosyl cyclaseの遺伝子構造." 生化学. 65. 1232 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 森泉茂樹: "reg遺伝子ファミリーに属する新しいヒト遺伝子(Human regIβ)の分離と染色体マッピング." 膵臓-日本膵臓学会誌(J.Jpn.Panc.Soc.). 18. 308 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 岡本宏: "Cyclic ADP-riboseをセカンドメッセンジャーとする新しい細胞内情報伝達系." 日本内分泌学会雑誌. 69. 168 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 加藤一郎: "ヒトVasoactive Intestinal Peptide(VIP)遺伝子を膵ランゲルハンス島β細胞内で過剰発現するトランスジェニックマウスの作製." 生化学. 65. 812 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 渡辺琢夫: "reg蛋白の膵ランゲルハンス島β細胞増殖作用." 生化学. 65. 1082 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 宮下曜: "ラットreg遺伝子の構造と発現調節機構." 生化学. 65. 1082 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 森泉茂樹: "新しい増殖因子“regファミリー"に属する二つのヒト遺伝子(regIα・regIβ)の構造・発現・染色体座位." 生化学. 65. 1082 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 那谷耕司: "ADP-ribosyl cyclase cDNA・遺伝子の構造と発現." 生化学. 65. 1088 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 高沢伸: "ヒトcyclic ADP-ribose合成系とその調節機構." 生化学. 65. 1088 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shao-Ying Hua.: "Cyclic ADP-ribose modulates Ca^<2+> release channels for activation by physiological Ca^<2+> entry in bullfrog sympathetic neurons." Neuron. in press. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 岡本宏: "膵β細胞破壊と防止についての岡本モデルとその展開." 糖尿病. 37. 99-102 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yonekura,H.: "Identification of the catalytically essential histidine of peptidylglycine monooxygenase." FEBS Letters. (投稿中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kato,I.: "Transgenic mice overexpressing human vasoactive intestinal peptide gene in pancreatic β-cells." Journal of Biological Chemistry. (投稿中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Okamoto,H.: "Mechanisms of destruction of insulin-secreting cells.in Nutrient Regulation of Insulin secretion." Flatt,P.R.,ed.(Portland Press,Colchester,England), 387-406 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Okamoto,H.: "Islet B-cell regeneration and reg genes.in Diabetes 1991" H.Rifkin,J.A.Colwell and S.I.Taylor,eds(Excepta Medica,Amsterdam), 268-270 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 米倉秀人: "PP(膵ポリペプチド)新生化学実験講座,第9巻,ホルモン" 日本生化学会編,(東京化学同人), 154-166 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Unno,M.: "Islet B-cell regeneration and reg genes.in Pancreatic Islet Cell Regeneration and Growth" Vinik,A.I.,ed.(Plenum Publishing Corp.), 61-66 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yonekura,H.: "Structue and expression of reg gene family.in Frontiers of Insulin Secretion and Pancreatic B-Cell Research" Flatt,P.R.,and Lenzan S.,eds(in press), (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takasawa,S.: "A cyclic ADP-ribose,a NAD+ metabolite,in stimulus-secretion coupling in pancreatic B-cells.in Frontiers of Insulin Secretion and Pancreatic B-Cell Research" Flatt,P.R.,and Lenzan S.,eds(in press), (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Noguchi,M.: "The source of oxygen atom in the α-hydroxyglycine intermediate of the peptidylglycine α-amidating reaction." The Biochemical Journal. 283. 883-888 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takasawa,S: "Cyclic ADP-ribose in insulin secretion from pancreatic cells." Science. 259. 370-373 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nata,K.: "Structure determination and evolution of the chicken cDNAand gene encoding prepropancreatic polypeptide" Gene. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kohri,K.: "Cloning and structural determination of human peptide YY cDNA and gene." Biochim. Biophys. Acta. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 安斎 高穂: "ヒト繊維芽細胞種NB-1におけるペプチドホルモンC末端アミド化酵素遺伝子の発現誘導." 生化学. 64. 865- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 米倉 秀人: "ペプチドホルモンC末端アミド化酵素活性中心.-部位特異的変異法を用いた解析-" 生化学. 64. 866- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Okamoto,H.: "Mechanisms of destruction of insulin-secreting cells.in Nutrient Regulation of Insulin secretion.(Flatr,P.R.,ed.)" Portland Press,Colchester,England., 387-406 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Unno,M.: "Islet B-cell regeneration and reg gene.in Pancreatic Islet Cell Regeneration and Growth.(Vinik,A.I.,ed.)" Plenum Publishing Crop.,New York,London,Washington DC,Boston, 61-66 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Noguchi,M,et al.: "The source of oxygen atom in the α-hydroxyglycine intermediate of the peptidylglycine α-amidating reaction." The Biochemical Journal. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Okamoto,H.,et al.(Yamamoto,S.,Nozaki,M.and Ishimura,Y.,eds.): "Peptide α-amidation is achieved by two distinct enzyme molecules encoded in a single mRNA." Proceeding of the Yamada Conference,Yamada Science Foundation,Osaka.27. 113-116 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Okamoto,H.,et al.: "The reaction mechanism and the enzymes concerned in the carboxyl-terminal α-amidation of VIP and related peptides." Biomedical Research. 3,Suppl.1. 3- (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 加藤 一郎,米倉 秀人,岡本 宏: "ペプチドC末端アミド化反応を触媒する2つの酵素は一本のmRNAより合成される." 生化学. 63. 1209-1213 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yonekura,H.,et al.: "Site-directed mutagenesis of rat peptidylglycine α-hydroxylase." The Journal of Biological Chemistry. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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