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Development of a bead-ELISA kit for rapid identification of enteric bacteria

Research Project

Project/Area Number 03557023
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TAKEDA Yoshifumi  Faculty of Medicine, Kyoto University, Proffessor, 医学部, 教授 (30029772)

Co-Investigator(Kenkyū-buntansha) OKU Yuichi  Nissui Pharmaceut.Co., Director, 開発研究部, 研究員
UESAKA Yoshihiko  nissui Pharmaceut.Co., Director, 開発研究部, 副主任研究員
HONDA Tetsuro  Nissui Pharmaceut.Co., Director, 中央研究所, 所長
KURAZONO Hisao  Faculty of Medicine, Kyoto University, Assistant, 医学部, 助手 (90186487)
NISHIBUCHI Mitsuaki  Faculty of Medicine, Kyoto University, Associate Professor, 医学部, 助教授 (50189304)
本田 徹朗  日水製薬株式会社, 中央研究所, 所長
山崎 伸二  京都大学, 医学部, 助手 (70221653)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥16,700,000 (Direct Cost: ¥16,700,000)
Fiscal Year 1992: ¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1991: ¥9,900,000 (Direct Cost: ¥9,900,000)
Keywordsbead-ELISA / cholera toxin / Campylobacter jejuni / ビ-ズELISA / 耐熱性溶血毒 / Vero毒素 / カンピロバクタ-・ジェジュニ / 迅速同定
Research Abstract

To diagnose bacterial infection, it is necessary to identify the causative bacteria as early as possible. However, conventional methods to identify bacteria by using culture methods takes at shortest 20-30 hours after obtaining clinical materials.
To evaluate a recently developed highly sensitive bead-ELISA to be used for a tool for the rapid identification of the causative bacteria of enteric infection, we cooperate with Institute of Cholera and Enteric Diseases of India and applied the bead-ELISA for stool specimens of cholera patients. The results showed that the bead-ELISA for cholera toxin is a rapid and sensitive method to detect cholera toxin, thus to diagnose cholera patient.
Bead-ELISA system was also developed by using a specific antigen and whole cell antigen to compylobacter jejuni. It was demonstrated that the a developed bead-ELISA is useful to identify Campylobacter jejuni. Thus an attempt will be made to develop a kit for identification of Campylobacter jejuni.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Yoshihiko UESAKA: "Detection of cholera toxin by a highly sensitive bead-enzyme linked immunosorbent assay" Microbiol.Immunol.36. 43-53 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Ramamurthy: "Evaluation of the bead-enzyme linked immunosorbent assay for detection of cholera toxin directly from stool specimens" J.Clin.Microbiol.30. 1783-1786 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Jun TADA: "Detection of the thermostable direct hemolysin gene (tdh) and the thermostable direct hemolysin gene (trh) of Vibrio parahaemolyticus by polymerase chain reaction" Mol.Cellular Probes.6. 477-487 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Jun TADA: "Non--isotopic microtitre plate-based assay for detecting products polymerase chain reaction amplification:Application to detection of the tdh gene of Vibrio parahaemolyticus" Mol.Cellular Probes.6. 489-494 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Amit Pal: "Reassessment of the prevalence of heat-stable enterotoxin (NAG-ST) among environmental Vibrio cholerae non-01 isolates using a NAG-ST DNA probe" Appl.Environ.Microbiol.58. 2485-2489 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Amit Pal: "Virulence traits of Aeromonas strains in relation to species and souce of isolation" Zentralblatta fur Bakteriologie. 276. 418-428 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yoshihiko Uesaka, Yoko Otsuka, Mitsuaki Kashida, Yuichi Oku, Kazuki Horigome, G.Balakrish Nair, S.C.Pal, Shinji Yamasaki, Mitsuaki Nishibuchi and Yoshifumi Takeda: "Detection of cholera toxin by a highly sensitive bead-enzyme linked immunosorbent assay." Microbiol. Immunol.36. 43-53 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Ramamurthy, S.K.Bhattacharya, Yoshihiko Uesaka, Kazuki Horigome, Mousumi Paul, D.Sen. Pal, Tae Takeda, Yoshifumi Takeda and G.Balakrish Nair: "Evaluation of the bead-enzyme linked immunosorbent assay for detection of cholera toxin directly form stool specimens." J.Clin.Microbiol.30. 1783-1786 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Jun Tada, Tetsuo Ohashi, Naoyuki Nishimura, Yoshinari Shirasaki, Hiroko Ozaki, Shigeru Fukushima, Jun Takano, Mitsuaki Nishibuchi and Yoshifumi Takeda: "Detection of thermostable direct hemolysin gene (tdh) and the thermostable direct hemolysin related hemolysin gene (trh) of Vibrio parahaemolyticus by polymerase chain reaction." Mol.Cellular Probes.6. 477-487 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Jun Tada, Tetsuo Ohashi, Naoyuki Nishimura, Hiroko Ozaki, Shigeru Fukushima, Jun Takano, Mitsuaki Nishibuchi and Yoshifumi Takeda: "Non-isotopic microtitre plate-based assay for detecting products of polymerase chain reaction amplification : Application to detection of the tdh gene of Vibrio parahaemolyticus." Mol.Cellular Probes. 6. 489-494 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Amit Pal, T.Ramamurthy, R.K. Bhadra, Tae Takeda, Toshio Shimada, Yoshifumi Takeda. G.Balakrish Nair, S.C.Pal and S.Chakrabarti: "Reassessment of the prevalence of heat-stable enterotoxin (NAG-ST) among environmental Vibrio cholerae non-O1 isolates using a NAG-ST DNA prode." Appl. Environ. Micoribol.58. 2485-2489 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Ramamurthy, Amit Pal, M.K. Bhattacharya, S.K.Bhattacharya, A.Saha Chowdhury, Yoshifumi Takeda, Tae Takeda, S.C.Pal and G.Balakrish Nair: "Serovar, biotype, phagetype, toxigenicity and antibiotic susceptibility patterns of Vibrio cholerae isolated during two consecutive cholera seasons (1989-90) in Calcutta." Indian J.Med. Res. A 95. 125-129 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Amit Pal, T.Ramamurthy, Asit R.Ghosh, Sudhir C.Pal, Yoshifumi Takeda and G.Balakrish Nair: "Virulence traits of Aeromonas strains in relation to species and source of isolation." Zentralblatta fur Bakteriologie. 276. 418-428 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yoshihiko Uesaka: "Detection of cholera toxin by a highly sensitive bead-enzyme linked immunosorbent assay" Microbiol.Immunol.36. 43-53 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Ramamurthy: "Evaluation of the bead-enzyme linked immunosorbent assay for detection of cholera toxin directly from stool specimens" J.Clin.Microbiol.30. 1783-1786 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Jun Tada: "Detection of the thermostable direct hemolysin gene (tdh) and the thermostable direct hemolysin gene (trh) of Vibrio parahaemolyticus by polymerase chain reaction" Mol.Cellular Probes.6. 477-487 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Jun tada: "Non-isotopic microtitre plate-based assay for detecting products polymerase chain reaction amplification:Application to detection of the tdh gene of Vibrio parahaemolyticus" Mol.Cellular Probes.6. 489-494 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Amit Pal: "Reassessment of the prevalence of heat-stable enterotoxin (NAG-ST) among environmental Vibrio cholerae non-01 isolates using a NAG-ST DNA probe" Appl.Environ.Microbiol.58. 2485-2489 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Amit Pal: "Virulence traits of Aeromonas strains in relation to species and souce of isolation" Zentralblatta fur Bakteriologie. 276. 418-428 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yoshihiko Uesaka: "Detection of cholera toxin by a highly sensitive beadenzyme linked immunosorbent assay" Microbiol.Immunol.36. 43-53 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiromasa Shirai: "Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae" J.Clin.Microbiol.29. 2517-2521 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 竹田 美文: "コレラ毒素の検出法" 検査と技術. 19. 532-536 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 竹田 美文: "病原細菌の迅速同定法としてのビ-ズELISAとPCR法" SRL宝函. 15(4). 23-28 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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