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Development of slit laser vital-microscope in the parenchymal organ

Research Project

Project/Area Number 03557040
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionUniversity of Tokyo

Principal Investigator

KAMIYA A.  Tokyo Univ Med Sch Professor, 医学部(医), 教授 (50014072)

Co-Investigator(Kenkyū-buntansha) 塩 育  (株)ニコンエンジニアリング, 取締役開発部長
AOKI T.  Tokyo Univ Med Sch Technician, 医学部(医), 教務職員 (00101113)
SHIBATA M.  Tokyo Univ Med Sch Instructor, 医学部(医), 助手 (60158954)
SHIO M.  Nikon Engineering Researcher
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1991: ¥7,400,000 (Direct Cost: ¥7,400,000)
KeywordsVital-microscope / Laser / Parenchymal organ / Microscopic tomography / Permeability / Microcirculation / 実質臓器 / 微小血管3次元像
Research Abstract

The purpose of this research is to develop the new fluorescent vital-microscope to observe the microcirculation of the deep field in the parenchymal organ and to quantify permeability parameters of a single capillary. To obtain the microvascular image in a chosen depth of the tissue, a pair of well-defined slit laser beams (28mum) were used which were focused so as to cross in a chosen depth of the tissue region and to excite the fluorescent tracer intensively at the crossing plane alone. The fluorescent image was visualized on a TV monitor through the microscope, of which the objective focus is adjusted to the crossing plane of slit beams. With this system it was possible to select the vertical zone of the microscopic image in the tissue by setting the crossing zone of slit beams at a desired level of the depth. To evaluate the efficiency of this microscope, we applied it to measure the capillary permeability of macromolecules in the rabbit tenuissimus muscle. The obtained values of permeability at the venular side showed higher than at the capillary area and the convective component of permeability was effective driving force for transcapillary exchange of macromolecules. From these results, it was confirmed that a newly developed microscope is useful to observe the deep field microcirculation without above and below the focal plane, and to clarify mechanisms of transcapillary exchange of macromolecules.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] M.SHIBATA and A.KAMIYA: "Blood flow dependence of local capillary permeability of Cr-EDTA in the rabbit skeletal muscle." Jpn.J.Physiol.42. 631-639 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.SHIBATA,T.TAKAHASHI,A.KAMIYA: "The mechanisms of transcapillary exchange of macromolecules." Microcirculation aannual. 8. 149-150 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.TAKAHASHI,M.SHIBATA,A.KAMIYA: "The concentrating mechanism of macromolecules in the rat mesentery collecting lymphatics during lymph transport." Microcirculation annual. 8. 151-152 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 柴田 政廣.高橋 龍尚,神谷 暸: "蛍光生体顕微鏡による巨大分子の毛細血管透過性計測法." 医用電子と生体工学. 31. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 柴田 政廣.高橋 龍尚,神谷 暸: "スリットレーザー蛍光生体顕微鏡を用いた実質臓器内微小循環の計測." 脈管学会誌. 33. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Shibata and A.Kamiya: "Blood flow dependence of local capillary permeability of Cr-EDTA in the rabbit skeletal muscle" Jpn. J. Physiol. 42. 631-639 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Shibata, T.Takahashi and A.Kamiya: "The mechanisms of transcapillary exchange of macromolecules" Microcirculation annual. 8. 149-150 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Takahashi, M.Shibata and A.Kamiya: "The concentrating mechanism of macromolecules in the rat mesentery collecting lymphatics during lymph transport" Microcirculation annual. 8. 151-152 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] A.Kamiya and J.Ando: "Vascular endothelial cell functions and biomechanics" Endothelium. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] A.Kamiya, H.Wakayama, K.Baba, T.Takahashi and M.Shitaba: "Optimality analysis of vascular-tissue system for oxygen transport in mammal" J. Theoretical Biol.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Shibata and A.Kamiya: "Blood flow dependence of local capillary permeability of Cr-EDTA in the rabbit skeletal muscle." Jpn.J.Physiol.42. 631-639 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Shibata,T.Takahashi,A.Kamiya: "The mechanisms of transcapillary exchange of macromolecules." Microcirculation aannual. 8. 149-150 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Takahashi,M.Shibata,A.Kamiya: "The concentrating mechanism of macromolecules in the rat mesentery collecting lymphatics during lymph transport." Microcirculation annual. 8. 151-152 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 柴田 政廣、高橋 龍尚、神谷 暸: "蛍光生体顕微鏡による巨大分子の毛細血管透過性計測法." 医用電子と生体工学. 31. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 柴田 政廣、高橋 龍尚、神谷 暸: "スリットレーザー蛍光生体顕微鏡を用いた実質臓器内微小循環の計測." 脈管学会誌. 33. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 柴田 政廣,神谷 暸 高橋 龍尚: "蛍光生体顕微鏡による実質臓器の微小血管透過性測定法" 脈管学. 31. 495-500 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Shibata,M.Yoshida,A.Kamiya: "Measurement of local capillary permeability in skeletal muscle by microscopic clearance method" Jpn.J.Physiol. 41. 725-734 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Shibata,A.Kamiya,T.Takahashi: "Measurements of single capillary permeability of macromolecules in the skeletal muscle by deep field laser vital-microscope(DFLM)" Med.Biol.Eng.& Comput.29(Suppl.). 260- (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Shibata,T.Takahashi,H.Wakayama,A.Kamiya: "In vivo measurements of single capillary permeability in the skeletal muscle by deep field laser microscope." Proc of Sth World Congress for microcirculation. 101- (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Shibata,A.Kamiya: "Blood flow dependence of local capillary permeability of Cr-EDTA in the rabbit skeletal muscle" Jap.J.Physiol.

    • Related Report
      1991 Annual Research Report
  • [Publications] 柴田 政廣,神谷 暸 高橋 龍尚: "深部組織観察用生体顕微鏡による単一毛細血管透過性の計測" 日本ME学会. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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