| Project/Area Number |
03557044
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Gifu University |
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Principal Investigator |
ORII Tadao Gifu University. Pediatrics. professor, 医学部, 教授 (20045339)
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| Co-Investigator(Kenkyū-buntansha) |
SUKEGAWA Kazuko Gifu University. Pediatrics. Instructor, 医学部, 助手 (60115409)
SUZUKI Yasuyuki Gifu University. Pediatrics. Assistant Professor, 医学部・附属病院, 講師 (00163014)
YAMAGUCHI Seiji Gifu University. Pediatrics. Assistant Professor, 医学部・附属病院, 講師 (60144044)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥10,900,000 (Direct Cost: ¥10,900,000)
Fiscal Year 1993: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1992: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1991: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | Inherited Metabolic Disease / Mucopolysaccharidosis / Mass Screening / DMB Method / Urinary Mucopolysaccharide / マススクリ-ニング |
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| Research Abstract |
We examined the direct quantitative method with 1,9-dimethylmethylene blue (DMB).
1. This method of quantifying excessive excretion of sulfated glycosaminoglycanes was suited to mass screening programs for mucopolysaccharidoses diseases.
2. It required only 12 ul of urine, and many speciments can be assayd in a short time.
3. Test results of the DMB method correlated with those of the CPC/carbazole-borate method.
4. A component in the diaper was highly reactive in the assay.
With this method, we anticipate that all newborns could be tested for MPS disease on a regular basis.
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