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ELECTRICAL AND CHEMICAL STIMULATION OF SPINAL CORD FOR TREATMENT OF URINARY DISTURBANCE

Research Project

Project/Area Number 03557068
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionDEPT.OF UROLOGY, AKITA UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

TSUCHIDA Seigi  Akita Univ., Dept.of Urology, Professor, 医学部, 教授 (00004549)

Co-Investigator(Kenkyū-buntansha) NOTO Hiromitsu  Akita Univ., Dept.of Urology, Assistant Prof., 医学部, 助手 (60164711)
NISHIZAWA Osamu  Akita Univ., Dept.of Urology, Assistant Prof., 医学部, 講師 (60091815)
HARADA Tadashi  Akita Univ., Dept.of Urology, Assistant Prof., 医学部, 講師 (00108953)
OGAWA Tetsurou  Akita Univ., 1st Dept.of Physiology, Professor, 医学部, 教授 (80004555)
鈴木 隆志  秋田大学, 医学部, 助手 (70179224)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥15,800,000 (Direct Cost: ¥15,800,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1992: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1991: ¥9,100,000 (Direct Cost: ¥9,100,000)
Keywordsmicturition / electrical stimulation / chemical stimulation / spinal cord / alpha-adrenergic mechanism / GABArgic mechanism / opiate / nitric oxide / アドレナリン作動性機構 / Naloxone / 排尿反射 / 電気刺激 / 化学刺激 / α刺激剤 / α遮断剤 / 脊髄 / GAVA / バクロフェン / ファクロフェン / セロトニン / 神経電気刺激 / 脊髄排尿中枢
Research Abstract

For the purpose of evaluation of the chemical stimulation of sacral spinal cord to modulate the micturition reflex, intrathecal administration of various drugs was performed using decerebrate dogs. GABA (0.1-0.3 mg), muscimol (0.01-0.2 mg), bacrofen (0.01-0.3 mg) increased bladder capacity 65%, 142%, 95% respectively. Phaclofen did not have a significant effect. Picrotoxin (0.05-0.3 mg) inhibited the effect of muscimol, and picrotoxin alone decreased bladder capacity 28%. These results indicate that there are both GABA-A and -B receptors in the sacral spinal cord, and that intrinsic GABA acts though GABA-A receptor. Intrathecal administration of phenylephrine (0.01-0.43 mg) increased bladder capacity and external urethral sphincter activity. Prazocin (0.01-0.3 mg) did not change bladder activity but decreased external urethral sphincter activity. Clonidine (0.07-0.32 mg) did not change bladder activity but decreased external urethral sphincter activity. Yohimbine (0.07-0.45 mg) decreas … More ed bladder contraction pressure. These results suggest that alpha-adrenergic mechanism in the sacral spinal cord increases bladder capacity and increases the external urethral sphincter activity through alpha-1 receptor, and increases bladder contractility and decreases external urethral sphincter activity through alpha-2 receptor.
Electrical stimulation of the sacral cord as well as sacral roots induced both bladder and urethral sphincter contractions. Bladder contraction was dominant when S-2 was stimulated while external urethral sphincter contraction was dominant when S-1 was stimulated. Potable stimulator (2.5 x 6 x 9 cm, 10-50 Hz, 0.2-1.0 msec duration, 0-50 V) was made for functional electrical stimulation. Electrical stimulation in the pontine micturition center (PMC) induced urethral relaxation as well as bladder contraction. Internal urethral sphincter relaxation was not inhibited by propranolol or atropine, but was abolished by hexamethonium or methylene blue. L-arginine increased bladder capacity and decreased bladder contraction pressure. These responses were partially reversed by methylene blue. Methylene blue enhanced bladder contraction evoked by PMC stimulation and induced spontaneous bladder contraction. These results indicate that internal urethral sphincter relaxations in mediated by non-adrenergic and non-cholinergic mechanism, and that L-arginine - NO pathway has a role in controlling the micturition reflex. Less

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] Noto,H.et al.: "Internal urethral sphincter relaxation mediated by a non-adrenergic and non-cholinergic mechanism in decerebrate cats." J.Urol.149. 341A (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shimoda,N.et al.: "Role of sacral alpha-adrenoceptive mechanisms in micturition reflex in the decerebrate dog." Proc.23rd ICS. 23. 108 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Noto,et al.: "Effect of L-arginine and methylene blue on the micturition reflex in decerebrate dogs." Proc.23rd ICS. 23. 99 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 佐藤敬悦: "橋排尿中枢による内尿道括約筋調節機構に関する研究" 日泌尿会誌. 84. 497-506 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 高橋徳男: "除脳イヌの反射性排尿におけるα-アドレナリン作動性機構に関する研究" 日泌尿会誌. 84. 711-719 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 西沢理: "脊髄内排尿機構とその障害" 排尿障害プラクティス. 1. 69-78 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 土田正義: "熊本悦明編,21世紀への泌尿器科学の展望,神経泌尿器科" ライフメディコム, 253(p102-111) (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tsuchida, S.: "Neurourology." Jap.J.Urol.81. 2007-2009 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sugaya, K., Nishizawa, O., Noto, H., Tsukada, T., Kohama, T., Shimoda, N.: "Identification of the regions of the pons that repond to baclofen, inaperisone and oxybutynin, which inhibit the micturition reflex." The autonomic nervous system. 28. 513-521 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sugaya, K., Nishizawa, O., Noto, H., Tsukada, T., Kohama, T., Shimoda, N., Harada, T.and Tsuchida, S.Morikawa, K.: "Identification of effective region of the pons in response to inaperisone which facilitates urine storage." Acta Urol.Jpn.37. 1639-1644 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nishizawa, O., Sugaya, K., Shimoda, N., Otomo, R.and Tsuchida, S.: "Role of the nucleus reticularis pontis oralis in the dorsolateral pontine tegmentum on micturition of cat." Neurourol.Urodyn.10. 363-364 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Noto, H., Roppolo, J.R., de Groat, W.C., Nishizawa, O., Sugaya, K.and Tsuchida, S.: "Opiate modulation of the micturition reflex at level of the pontine micturition center." Urol.Int.47(Suppl.1). 19-22 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tsukada, T., Noto, H., Nishizawa, O.and Tsuchida, S.: "Gabanergic modulation of the micturition reflex at the level of the sacral spinal cord in decerebrate dogs." Neurouro.Urodyn.10. 366-367 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Noto, H.: "Inhibitory modulation of the micturition." Jap.J.Urol.83. 808-811 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Noto, H., Nishizawa, O.and Tsuchida, S.: "Micturition. -functional anatomy and physiology-" Brain Nursing. 8. 865-871 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kohama, T.: "Neuroanatomical studies on the urine storage facilitatory areas in the cat brain. Part I.Input teuronal structures to the nuleus locus subcoeruleus and the nucleus reticularis pontis oralis." Jap.J.Urol.83. 1469-1477 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kohama, T.: "Neuroanatomical studies on pontine urine storage facilitatory areas in the cat brain. Part II.Output neural structure from the nucleus locus subcoeruleus and the nucleus reticularis pontis oralis." J.Urol.83. 1478-1483 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Satoh, K., Noto, H., Nishizawa, O., Sugaya, K., Suzuki, T., Shimoda, N., Otomo, R.and Tsuchida, S.: "Brainstem control of the internal and external urethral sphincter activity in decerebrate dog." J.Urol.147. 348A (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Satoh, K.: "Pontine control of the internal urethral sphincter activity on decerebrate dogs." Jap.J.Urol.84. 497-506 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takahashi, T.: "Alpha adrenergic mechanism on the reflex micturition in the male decerebrate dog." Jap.J.Urol.84. 711-719 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nishizawa, O., Sugaya, K., Suzuki, T., Satoh, K., Kohama, T., Shimoda, N., Otomo, R., Harada, T., Tsuchida, S., Mori, S.: "Effect of YM-12617 (AMSULOSIN HYDROCHLORIDE) on lower urinary tract function in the female decerebrate dog." Acta Urol.Jan.38. 891-895 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Noto, H., Fujieda, N., Sasaki, R., Kawahara, T., Shimoda, N., Nishizawa, O., Harada, T., Tsuchida, S.and Yamagishi, R.: "Effect of L-arginine and methylene blue on the micturition reflex in decerebrate dogs." Proc.23rd ICS. 23. 99 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kawahara, T., Nishizawa, O., Noto, H., Shimoda, N., Suzuki, K., Fujieda, N., Yamashiro, S., Harada, T.and Tsuchida, S.: "Effect of the inhibitors of nitric oxide/cyclic GMP pathway on the internal urethral relaxation in anesthetized rats." Proc.23rd ICS. 23. 102 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shimoda, N., Noto, H., Satoh, K., Nishizawa, O., Harada, T.and Tsuchida, S.: "Role of sacral alpha-adrenoceptive mechanisms in micturition reflex in the decerebrate dog." Proc.23rd ICS. 23. 108 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Noto, H., Kawahara, T., Shimoda, N., Suzuki, T., Nishizawa, O., Harada, T.and Tsuchida, S.: "Internal urethral sphincter relaxation mediated by a non-adrenergic and non-cholinergic mechanism in decerebrate cats." J.Urol.149. 341A (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Harada,T.,et al.: "The effect of alpha-2 agonists and antagonists on the lower urinary tract of the rat." J.Urol.149. 159-164 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Noto,H.,et al.: "Internal urethral sphincter relaxation mediated by a non-adrenergic and non-cholinergic mechanism in decerebrate cats." J.Urol.149. 341A (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nishizawa,O.,et al.: "Beta-adrenergic subtype function on the vesicourethral activity of the rat." Proc.23rd ICS. 23. 274 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shimoda,N.,et al.: "Role of sacral alpha-adrenoceptive mechanisms in micturition reflex in the decerebrated dog." Proc.23rd ICS. 23. 99 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 佐藤 敬悦: "橋排尿中枢による内尿道括約筋調節機構に関する実験的研究" 日泌尿会誌. 84. 497-506 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 西沢 理: "脊髄内排尿機構とその障害" 排尿障害プラクティス. 1. 69-78 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 土田 正義: "熊本悦明編,21世紀への泌尿器科学の展望,神経泌尿器科." ライフメディコム, 253(p102-111) (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 土田 正義: "腎盂,尿管機能" 中本総合印刷, 174 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nishizawa,O.,Sugaya,K.,Satoh,K.,Shimada,N.and Tsuchida,S.: "Alpha 1 adrenergic function on the micturition cycle in the female decerebrate dog." J.Urol.147. 348A- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Satoh,K,.Noto,H.,Nishizawa,O.,Sugaya,K.,Suzuki,T.,Shimada,N.,Otomo,R.and Tsuchida,S.: "Brainstem control of the internal and external urethral sphincter activities in decerebrate dog." J.Urol.147. 348A- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nishizawa,O.,Sugaya,K.,Takahashi,T.,Shimada,N.,Noto,H.,Harada,T.and Tsuchida,S.: "Alpha-2 adrenergic effect on the vesicourethral function of the male decerebrate dog." Neurouro Urodyn. 11. 350-352 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 土田 正義.西沢 理 能登 宏光: "機能からみた解剖・生理ー排泄する(排尿)ー" ブレインナーシング. 8(10). 865-871 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 小浜 丈夫(大学院): "ネコの橋内蓄尿促進部位に関する神経解剖学的研究 第1報青斑下核および吻側橋網様核への入力神経経路について" 日泌尿会誌. 83(9). 1469-1477 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 小浜 丈夫(大学院): "ネコの橋内蓄尿促進部位に関する神経解剖学的研究 第2報青斑下核および吻側橋網様核への出力神経経路について" 日泌尿会誌. 83(9). 1478-1483 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 土田 正義.能登 宏光: "病因と分類 泌尿器科MOOK尿失禁治療のキーポイント" 金原出版, 28-39 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nishizawa,O.,Sugaya,K.,Shimoda,N.,Otomo,R.,: "Role of the nucleus reticularis pontis oralis in the dorsolateral pontine tegmentum on micturition of cat." Neurourology and Urodynamics. 10. 363-364 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Noto,H.,Nishizawa,O.,Sugaya,K.,Shimoda,N.,and Tsuchida,S.: "Urodynamic evaluation of the effect of centrally acting muscle relaxant inaperisone hydrochloride on the micturition reflex in the decerebrate dog." Neurourology and Urodynamics. 10. 364-366 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tsukada,T.,Noto,H.,Nishizawa,O.and Tsuchida,S.: "GABAergic modulation of the micturition reflex at the level of the sacral spinal cord in decerebrate dogs." Neurourology and Urodynamics. 10. 366-367 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nishizawa,O.,Sugaya,K.,Satoh,K.,Shimoda,N.and Tsuchida,S.: "Alpha 1 adrenergic function on the micturition cycle in the female d decerebrate dog." Journal of Urology. 147. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Satoh,K.,Noto,H.,Nishizawa,O.,Sugaya,K.,Suzuki,T.,et al.: "Brainstem control of the internal and external urethral sphincter activities in decerebrate dogs." Journal of Urology. 147. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 土田 正義: "神経泌尿器科学" カレント・テラピ-. 10. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 土田正義編,土田 正義・他著: "尿失禁治療のキ-ポイント" 金原出版, (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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