A development of repetitious mechanical stress loading system for cultured cells.
| Project/Area Number |
03557078
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
YAMADA Shoji Showa Univ., School of Dentistry, Professor, 歯学部, 教授 (00111617)
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Takanobu Showa Univ., School of Medicine, Lecturer, 医学部, 講師 (70245877)
SAITO Shigeru Showa Univ., School of Dentistry, Assistant, 歯学部, 助手 (20195986)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1993: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1992: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥3,900,000 (Direct Cost: ¥3,900,000)
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| Keywords | OSTEOBLAST / PERIODONTAL LIGAMENT / CELL CULTURE / MECHANICAL STRESS / REPETITIOUS STRETCH / CELL DIVISION / COLLAGEN GEL / ALKALINE PHOSPHATASE / コラ-ゲンゲル |
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| Research Abstract |
At the start of project, we obtained the basic dotards for an appropnate strenght and cycle of mechanical stress to load on bone cells using our hand-made stress loading system. During an endurance test of parts, we realized that the solenoid driving system is not durable in CO_2 incubator, and we change the system to waterproof motor driving-cam transmitting system. This change brought some merits besides a durability. An accelerating process in reciprocating movement became controllable with changing a shape of cam, which was impossible for solenoid driving system. A manufacture for trial and reform were repeated for a year and the system was completed as a machine with features,
1. A repetitious mechanical stress is loaded for cells embedded in thin layr of collagen gel by stretching a nylon mesh, which is a reinforcement for cllagen gel. Not only a distance of stretch and cycle but also an accelerating process in reciprocating movement is controllable with changing the speed of motor and the shape of cam.
2. A collagen gel layr is thin enough (about 1 mm) to neglect the effects of increasing permeability of nutrition into the gel according a repetitious stretch. Moreover, you can set a sample for cyclic control, which moves in the medium reciprocally without any stretch, or a sample for stable control, which standstill in the medium. These features make it possible to distinguish the true effect of mechanical forth.
3. A direct microscopic observation of cells in collagen gel layr during the load of stress is possible. This makes it possible to measure the amount of forth loaded on cells as an amount of deformation of cells, and to compare the data obtained from different machines.
It was confirmed by an endurance test and two years use for test that this system has a good quality.
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Report
(4 results)
Research Products
(3 results)
