| Project/Area Number |
03557086
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
外科・放射線系歯学
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| Research Institution | The Department of Oral and Maxillofacial Surgery 2, Faculty of Dentistory, Tokyo Medical and Dental University |
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Principal Investigator |
ENOMOTO Shoji Tokyo Medical and Dental University, Dentistory, Professor, 歯学部, 教授 (40013940)
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| Co-Investigator(Kenkyū-buntansha) |
IMAI Yoji Tokyo Medical and Dental University, Institute for Medical and Dental Engineerin, 医用器材研究所, 教授 (50013975)
MIYATA Teruo Koken Bioscience Institute, Director, 日本医用高分子研究所, 所長
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 1992: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1991: ¥8,700,000 (Direct Cost: ¥8,700,000)
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| Keywords | Biodegradable / Bone substitute materials / Bone Morphogenetic Protein : BMP / Poly-L-Lactic Acid / Porus / Recovery of bone defect / Calcium content / Liquid chromatography / 頭骨再建 / 吸収性人工骨 / ポリ乳酸-グリコ-ル酸 / ハイドロキシアパタイト / True Bone Ceramics(TBC) / アテロコラ-ゲン |
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| Research Abstract |
There are many cases to make bone defects in the oral and maxillo-facial region, for example cysts, cancers and so on. So Bone Morphogenetic Protein (BMP) is needed for clinical usage. And a lot of studies have been done to purify the BMP and to investigate the physiological function of that. Several reports suggested the possibility that BMP (Osteogenin, BMP-3 from demineralized bone matrix have stronger osteoinductive activity rather than rhBMP -2. Therefore the BMP was purified from the demineralized bone extract by gel filtration on Sephacry1 S-200HR, cation exchange with Mono-s, Heparin affinity column and finally by c1/8 reverse phase chromatography. The BMP preparation obtained in this study induced ectopic bone and marrow tissue in ectopic site in 2 weeks after implantation into the thoracic subcutaneous tissue. The results of 15% SDS-PAGE revealed the molecular weight in reduced condition is half that in non-reduced condition as reported before. Peptide sequence analysis revealed that the purified BMP fraction contained "BMP-3" reported by Wozney et al.
A skull defect which is 8 mm in diamenter was prparated, and porus poly-L-lactic acid disc including the BMP was implanted into the defect. Osteoinductive activity induced by the disc was evaluated by means of soft X-ray image, calcium content, and histological observation. 2 weeks after implantation, bone tissue was induced and large value of calcium was detected in the excised discs. 4 weeks after implantation, also bone tissue was induced and larger value of calcium was detected from the excised discs. These results supported that the porus poly-L-lactic acid disc will be effective for recovery of bone defect.
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