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Analyzer or photo-emitting biomaterials

Research Project

Project/Area Number 03557105
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionHokkaido University

Principal Investigator

KOYAMA Tomiyasu  Hokkaido Univ., Res.Inst.Electr.Sci., Prof., 電子科学研究所, 教授 (50001681)

Co-Investigator(Kenkyū-buntansha) ARAISO Tsunehisa  Hokkaido Univ., Res.Inst.Electr.Sci., As.Prof., 電子科学研究所, 助教授 (30151145)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1991: ¥5,100,000 (Direct Cost: ¥5,100,000)
KeywordsPhoto-emitting biomaterials / Molecular oscillation / Phospholipids / Phosphorous headportion / Glycerol skeleton / Phase transition / Hexagonal II phase / Langmuir-Blodget membrane / LB膜 / 傾斜角 / 揺動角 / 拡散速度 / 脂質二重層 / ヘキサゴナルII相(HII) / ジアシルグリセロール / 回転緩和時間 / 顕微鏡式時間分解蛍光偏光解消計 / ピコ秒時間分解蛍光計 / 燐脂質二重層 / 燐脂質頭部の運動 / 極微弱発光
Research Abstract

A new system for analyzing photo-emitting biomaterials was constructed with a sensitive photodetector, optic devices and mode-locked pulsed laser. Applications of this system were made for the analysis of the dynamic microstructure of suspended and fixed materials.
1. Fluorescent particles accumulating in cardiac tissues of aged rats were collected from ventricular tissues and suspended in salt solution. The obtained results suggested that the size of fluorescent component was only several hundred Daltons and oscillated in a wide range.
2. Suspension studies on liposomes, which are experimental models for biomembranes, showed that the head groups and the glycerol skeletons of phospholipids oscillated with a rotational diffusion constant of the order of 10^<-7>/sec.
3. Interesting results were obtained on the transition of bilayr to hexagonal phases of phosphlipid layrs. The phase transition induced by incubation of lipisomes with diacylglycerol produced a decrease in head group oscillation and an increase in fatty acyl chain movement, indicating curvature changes of lipid layrs.
4. A new idea of fractioned polarization was proposed for the analysis of the polarized light emitted from fixed materials. With the aids of this idea, the main angle of obliquely standing phospholipid molecules, wobbling angle around the main angle and rotational diffusion constant could be calculated from the polarized fluorescent light emitted from fluorophore-labeled Langmuir-Blodget lipid membranes.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Saito H,Araiso T,Shirahama H,Koyama T: "Dynamics of the bilayer-water interface of phospholipid vesicles and the effect of cholesterol:a picosecond fluorescence anisotropy study" J Biochem. 109. 559-565 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kimura N,Tsuneta R,Araiso T et al.: "Fluorescence depolarization to define wobbling motion of oriented molecules in Langmuir-Blodgett films" Chemistry and Physics of Lipids. 57. 39-47 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mukasa K,Araiso T,Kimura N: "Estimation of molecular motion in the LB film using time-resolved fluorescence depolarization in order to control the film structure" New Functionality Materials,vol C,Elsvier Science Publishers. 705-710 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 長沼睦雄: "ジアシルグリセロールにより誘導されるリポソーム膜における液晶-逆ヘキサゴナル相転移の蛍光分光学的解析。" 北海道医学雑誌. 69. 65-71 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 荒磯恒久: "膜脂質の流動性と膜タンパク質の動態。" 膜. 19. 3-13 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kimura N,Tsuneta R,Araiso T,Mukasa K: "Time-resolved fluorescence depolarization to study the molecular motion of organic thain films" Submitted to the journal “Langmuir".

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Koyama T,Araiso T,Jin T: "Subnanosecond analysis of the fluorescent component of pigments accumulating in rat cardiac muscle" in preparation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Saito H, Araiso T, Shirahama H, Koyama T: "Dynamics of the bilayr-water interface of phospholipid vesicles and the effect of cholesterol : a picosecond fluorescence anisotropy study." Journal of Biochemistry. 109. 559-565 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kimura N, Tsuneta R, Araiso T et al.: "Fluorescence depolarization to define wobbling motion of oriented molecules in Langmuir-Blodgett films." Chemistry and Physics of Lipids. 57. 39-47 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mukasa K, Araiso T, Kimura N: "Estimation of molecular motion in the LB film using time-resolved fluorescence depolarization in order to control the film structure." New Functionality Materials. (Elsvier Science Publishers). vol V. 705-710 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Naganuma M: "Fluorometric analysis of liquid-crystalline to inverted hexagonal phase transition induced by diacylglycerol in liposomes" Hokkaido Journal of Medical Science (in Japanese). 69. 65-71 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Araiso T: "Fluidity in membrane lipid layr and protein dynamics." Membrane (in Japanese). 19. 3-13 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kimura N, Tsuneta R, Araiso T, Mukasa K: "Time-resolved fluorescencs depolarization to study the molecular motion of organic thin films." the journal "Langmuir". (submitted to).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Koyama T, Araiso T, Jin T: "Subnanosecond analysis of the fluorescent component of pigments accumulating in rat cardiac muscle." (in preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mukasa K,Araiso T,Kimura N: "Estimation of molecular motion in the LB film using time-resolved fluorescence depolarization in order to control the film structure" New Functionality Materials,vol C,Elsvier Science Publishers. 705-710 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 荒磯恒久: "膜脂質の流動性と膜タンパク質の動態" 膜. 19. 3-13 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 荒磯 恒久: "蛍光偏光法による燐脂質二重層のグリセロール骨格部の運動測定" 第69回日本生理学会大会予稿集. P231 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Araiso Tsunehisa: "A fluorescence dpolarization study of the motion of the glycerol skeletal moiety in phospholipid bilayer" Jpn J Physiol Suppl. 42. P297 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 長沼 睦雄: "ジアシルグリセロールによって誘導される膜融合における燐脂質分子運動の変化の蛍光法による解析" 第72回北海道医学大会生理分科会抄録集. P21 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 荒磯 恒久: "ジアシルグリセロールによって誘導される膜融合における燐脂質分子運動変化" 日本生物物理学会第30回年会講演予稿集. S54 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Saito H.,Araiso T.Shirahama H.Koyama T.: "Dynamics of the bilayer - Water interface of phospholipid vesicles and the effect of cholesterol : A picosecond fluorescence anisotropy study." J.Biochem.109. 559-565 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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