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DEVELOPMENT OF A BIOSUPRAMOLECULAR NMR SYSTEM WITH A SUPERCONDUCTING MAGNET

Research Project

Project/Area Number 03558029
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 生物物性学
Research InstitutionYOKOHAMA NATIONAL UNIVERSITY (Y. N. U.)

Principal Investigator

AKUTSU Hideo  Y. N. U., Faculty of Engineering, Professor, 工学部, 教授 (60029965)

Co-Investigator(Kenkyū-buntansha) KURODA Yukio  Bruker Japan, Dpt of Technical Service, Head, 技術サービス部, 部長
NIKI Katsumi  Y. N. U., Faculty of Engineering, Professor, 工学部, 教授 (00017899)
河合 剛太  東京大学, 工学部, 助手 (70211860)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 1992: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥11,300,000 (Direct Cost: ¥11,300,000)
KeywordsSolid-State NMR / Biosupramolecular System / Deuterium NMR / Solid-State Probe head / Deuterated Phospholipids / Superconducting Magnet NMR / 固体用プロ-ブ
Research Abstract

A multinuclear observation system was built into our AM-400 NMR spectrometer (Bruker). Then, a solid-state probe head for deuterium NMR was developed. The quadrupole echo pulse sequence was used for the measurements. At first, a solenoid coil was built in a probe head, originally used for solution NMR. The 90゚ pulse was 12mus. The circuits of the probe head and matching box were simplified. After the modification, the 90゚ pulse became to be about 4mus. Thus, we have established the solid-state NMR system, which can be used for the start point of this project. phospholipids specifically deuterated in the glycerol backbone were obtained from E. coli cells cultured in the presence of perdeuterated glycerol.
Since biological samples contain water, the sample tube should be held vertically. Although the power is very efficient in the solenoid coil, the sample tube is kept in horizontal. So we decided to change the coil design. A saddle type coil was attached on the surface of a glass tube with 5 mmphi, which was put in the vertical direction. The 8-turn coil fits to 0.94 cm^3 sample volume. The 90゚ pulse of this coil was 7.8mus. In order to get more power, a high power amplifier was connected. However, arching took place in the probe head on the use of this amplifier. Since the bore size of our magnet is narrow, the probe is not enough to avoid the arching. The probe was examined without the amplifier. By using 60 mg deuterated phospholipids, a ^2H-NMR spectrum in good quality was obtained. The spin-lattice relaxation time was measured using the inversion-recovery method. Whole region of the power pattern spectrum was inverted by the 180゚ pulse. These results show that this coil can be used for the investigation of the phospholipid bilayers. Thus, we have succeeded in establishing a solid-state NMR system suitable for biomembrane studies.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] H.AKUTSU,T.NAGAMORI: "Conformational analysis of the polar head groapin phosphatidgl bila ers" 30. 4510-4516 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 日本生化学会編: "新生化学実験講座「生体膜と膜輸送上」" 東京化学同人, 424 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 水島・宇井編: "バイオメンブレンシステム-生体膜と生命現象" 講談社, 182 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H. Akutsu and T. Nagamori: "Conformational analysis of the polar head group in phosphatidylcholine bilayers." Biochemistry. VOL. 30. 4510-4516 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Akutsu,T.Nagamori: "Conformational analysis of the polar headgroupin phosphatidylcholine bilayers" Biochemistry. 30. 4510-4516 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 日本生化学会編: "新生化学実験講座6「生体膜と膜輸送上」" 東京化学同人, 424 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 水島・宇井編: "バイオメンブレンシステム-生体膜と生命現象" 講談社, 182 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Akutsu,T.Nagamori: "Conformational analysis of the polar head groupin phosphatidgecholine bilayers:A structural change induced by cations." Biochemistry. 30. 4510-4516 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] J.-S.Park,K.Kano,Y.Morimoto,H.Higuchi,N.Yasuoka,M.Ogata,K.Niki and H.Akutsu: "HNMR studies on ferricytochrome c_3 from D.unlgaris Miyazaki F and its interaction with terredoxin I" J.Biomol.NMR,. 1. 271-282 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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