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A study on the temperature-dependent modes of selective transmission of mitochondria in Drosophila

Research Project

Project/Area Number 03640538
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 遺伝学
Research InstitutionOchanomizu University

Principal Investigator

MATSUURA Etsuko T.  (Instructor) Dept. of Biology Ochanomizu University, 理学部, 助手 (00111691)

Co-Investigator(Kenkyū-buntansha) NIKI Yuzo  (Associate Professor) Dept. of Biology Ibaraki University, 理学部, 助教授 (00134164)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsDrosophila / Mitochondrial transmission / Mitochondrial DNA / Heteroplasmy / ヘチロプラズミー / ヘテロプラズミ-
Research Abstract

The temperature-dependent modes of selective transmission of mitochondrial DNA (mtDNA) were investigated in heteroplasmic lines which were established by germ-plasm transplantation in Drosophila melanogaster. The effects of the nuclear genome on the temperature- dependency were demonstrated in two sets of heteroplasmic lines: one established by reciprocal transplantation of germ plasm in D. melanogaster and the other derived from two D. melanogaster heteroplasmic lines possessing D. mauritiana mtDNA in which the nuclear genomes were reciprocally substituted. In each set, the temperature- dependent modes of selective transmission of the same two types of mtDNA were different depending on the nuclear genomes. Involvement of propagation of mitochondria and/or mtDNA replication in selective transmission was suggested from three observations, using heteroplasmic lines with appropriate combinations of two types of mtDNA and the nuclear genome. First, the intensity of selection was strongly affected by the temperature at the stage of oogenesis among the various developmental stages. Secondly, the intensity of selection was affected by some nucleotide sequences in the A+T-rich region containing the origin of replication. Thirdly, in heteroplasmic lines possessing two different sizes of mtDNA, the smaller one was selectively transmitted. In the last two cases,the effects of temperature were not clear. These results indicate that the nuclear genome is involved in determining the temperature-dependent mode of the selective transmission of mtDNA. They also suggest that the selective transmission is based on the differences in mtDNA replication. The present results will greatly contribute to clarify the molecular mechanisms of the regulation of mitochondrial transmission in eucaryotic cells.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] MATSUURA,E.T.,FUKUDA,H.and CHIGUSA,S.I.: "Mitochondrial DNA heteroplasmy maintained in natural populations of Drosophila simulans in Reunion." Genetical research. 57. 123-126 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] MATSUURA,E.T.,NIKI,Y.and CHIGUSA,S.I.: "Selective transmission of mitochondrial DNA in heteroplasmic lines for intra- and interspecific combinations in Drosophila melanogaster." Japanese Journal of Genetics. 66. 197-207 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] NAGATA,Y.and MATSUURA,E.T.: "Temperature-dependency od electron-transport activity in mitochondria with exogenous mitochondrial DNA in Drosophila." Japanese Journal of Genetics. 66. 255-261 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TSUJIMOTO,Y.,NIKI,Y.and MATSUURA,E.T.: "Further study on selective transmission of mitochondrial DNA in heteroplasmic lines of Drosophila melanogaster." Japanese Journal of Genetics. 66. 609-616 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] MATSUURA,E.T.: "Selective transmission of mitochondrial DNA in Drosophila." Japanese Journal of Genetics. 66. 683-700 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KONDO,R.,MATSUURA,E.T.and CHIGUSA,S.I: "Further obseravation of paternal transmission of Drosophila mitochondrial DNA by PCR selective amplification method." Genetical Research. 59. 81-84 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsuura,E.T.: "Mitochondrial transmission in Drosophila.pp.213-221 in mechanisms of Molecular Evolution edited by N.Takahata and A.G.Clark" Japan Scientific Societies Press, Tokyo and Sinauer, Sunderland,U.S.A., 1993, 250 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsuura, E. T., Fukuda, H. and Chigusa, S. I.: "Mitochondrial DNA heteroplasmy maintained in natural populations of Drosophila simulans in Reunion." Genetical Research. 57. 123-126 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsuura, E. T., Niki, Y. and Chigusa, S. I.: "Selective transmission of mitochondrial DNA in heteroplasmic lines for intra- and interspecific combinations in Drosophila melanogaster." Japanese Journal of Genetics. 66. 197-207 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nagata, Y. and Matsuura, E. T.: "Temperature-dependency of electron-transport activity in mitochondria with exogenous mitochondrial DNA in Drosophila." Japanese Journal of Genetics. 66. 255-261 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tsujimoto, Y., Niki, Y. and Matsuura, E. T.: "Further study on selective transmission of mitochondrial DNA in heteroplasmic lines of Drosophila melanogaster." Japanese Journal of Genetics. 66. 609-616 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsuura, E. T.: "Selective transmission of mitochondrial DNA in Drosophila." Japanese Journal of Genetics. 66. 683-700 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kondo, R., Matsuura, E. T. and Chigusa, S. I.: "Further observation of paternal transmission of Drosophila mitochondrial DNA by PCR selective amplification method." Genetical Research. 59. 81-84 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsuura, E. T.: "Mitochondrial transmission in Drosophila." Mechanisms of Molecular Evolution, edited by N. Takahata and A. G. Clark. Japan Scientific Societies Press, Tokyo and Sinauer, Sunderland, U.S.A.213-221 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kondo, R.,Matsuura,E.T. and Chigusa, S.I.: "Further observation of paternal transmission of Drosophila mitochondrial DNA by PCR selective amplification method." Genetical Research. 59. 81-84 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Matsuura, E.T.: "Mitochondrial transmission in Drosophila.pp.213-221 in Mechanisms of Molecular Evolution edited by N. Takahata and A.G. Clark" Japan Scientific Societies Press, Tokyo and Sinauer, Sunderland, U.S.A., 250 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Matsuura,E.T.: "Mitochondrial DNA heteroplasmy maintained in natural populations of Drosophila simulans in Reunion." Genetical Research. 57. 123-126 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Matsuura,E.T.: "Selective transmission of mitochondrial DNA in heteroplasmic lines for intraーand interspecific combinations in Drosophila melanogaster." Japanese Jouranal of Genetics. 66. 197-207 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nagata,Y.: "Temperatureーdependency of electronーtransport activity in mitochondria with exogenous mitochondrial DNA in Drosophila." Japanese Journal of Genetics. 66. 255-261 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tsujimoto,Y.: "Further study on selective transmission of mitochondrial DNA in heteroplasmic lines of Drosophila melanogaster." Japanese Journal of Genetics. 66. 609-616 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kondo,R.: "Further observation of paternal transmission of Drosophila mitochondrial DNA by PCR selective amplification method." Genetical Research. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Matsuura,E.T.: "Selective transmission of mitochondrial DNA in Drosophila." Japanese Journal of Genetics. 66. 683-700 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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