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A STUDY OF IDENTIFICATION AND QUANTIFICATION OF MIDDLE REPETITIVE SEQUENCES OF DROSOPHILA GENOME

Research Project

Project/Area Number 03640540
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 遺伝学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

HARADA Ko  KYUSHU UNIV. SCIENCE,ASSISTANT, 理学部, 助手 (40150396)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsmiddle repetitive sequence / Drosophila melanogaster / transposable elements / random clone / in situ hybridilzation / genome structure / in situ hybridization / ランダムクロ-ン / オナジショウジョウバエ
Research Abstract

In order to study the genome structure concerning to the repetitive sequences we mapped random clones extracted from the genomic library of Drosophila melanogaster on the salivary gland chromosome. About 800 clones were randomly chosen from the phage library and chromosomal locations were determined for 231 clones by in situ hybridization. 142 clones were mapped on the unique locations and 89 clones were mapped on the multiple chromosomeal locations. Two groups of clones were distinguished from the distribution of these clones containing sequences which have multiple copies on the chromosome. One group is made of clones which has relatively small copy numbers (2-6) and the others have more than 10 copies on the chromosome. Mapping these clones to the chromosome of Drosopila simulans, the closely related species of D.melanogaster, it was found that the former group of clones were mapped on the same location and/or have the same copy numbers. On the other hand, the latter had generally smaller copy numbers and were mapped on different locations. These results suggest that the former clones are made of mainly multigene families and the latters contains transposons. We are now analyzing detailed molecular structure of these clones.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Harada,K.,Kusakabe,S.and Mukai,T.: "Comparative study of P element activity in two natural populations of Drosophila melanogaster" Japn.J.Genet.66. 725-737 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Harada,K.,Kusakabe,S.,Yamazaki,T.and Mukai,T.: "Spontaneous mutation rates in null and band-morph mutaitons of enzyme loci in Drosophila melnogaster." Genetics.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yamaguchi,Y.,Harada,K.,Takano,T.S.and Yamazaki,T.: "Molecula Analysis of Spontaneous Gpdh null mutations in Drosophila melanogaster." Genetlcs.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Harada,K.,Kusakabe,S.and Mukai,T.: "Comparative study of P element activity in two natural populations of Drosophila melanogaster" Japn.J.Genet. 66. 725-737 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Harada,K.,Kusakabe,S.,Yamazaki,T.and Mukai,T.: "Spontaneous mutation rates in null and band-morph mutaitons of enzyme loci in Drosophila melnogaster." Genetics.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yamaguchi,Y.,Harada,K.,Takano,T.S.and Yamazaki,T.: "Molecula Analysis of Spontaneous Gpdh null mutations in Drosophila melanogaster." Genetlcs. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Harada,K.,Kusakabe,S.and Mukai,T.: "Comparative study of P element activity in two natural populations of Drosophila melanogaster" Japn.J.Genet.66. 725-737 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Harada,K.,Kusakabe,S.,Yamazaki,T.and Mukai,T: "Spontaneous mutation rates in null and band-morph mutaitons of enzyme loci in Drosophila melnogaster." Genetics.

    • Related Report
      1992 Annual Research Report
  • [Publications] Yamaguchi,Y.,Harada,K.,Takano,T.S.and Yamazaki,T.: "Molecula Analysis of Spontaneous Gpdh null mutations in Drosophila melanogaster." Genetlcs.

    • Related Report
      1992 Annual Research Report
  • [Publications] Harada,K.: "Comparative of P element activity in two natural populations of Drosophila melanogaster." Japanese Journal of Genetics. 66. 725-737 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Harada,K.: "Genetic variation in glucose 6ーphosohogluconate dehydrogenase activities induced by spontaneous modifying mutations in Drosophila melanogaster." Genetical Research.

    • Related Report
      1991 Annual Research Report
  • [Publications] Harada,K.: "Spontaneous mutation rates in null and bandーmorph mutations of enzyme loci in Drosophila melanogaster." Proc.Natl.Acad.Sci USA.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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