| Project/Area Number |
03640560
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
生態学
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| Research Institution | National Institute for Environmental Studies |
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Principal Investigator |
KOHATA Kunio National Institute for Environmental Studies Regional Environment Division Senior Researcher, 地域環境研究グループ, 主任研究員 (30132848)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | carotenoid / chlorophyll a / chlorophyll a contribution / high-performance liquid chromatography / HPLC / photosynthetic pigments / phytoplankton / 網別存在量 / 高速液体クロマトグラフィ- |
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| Research Abstract |
Algal photosynthetic pigments, chlorophyll (Chl) a, Chl c and carotenoids were determined by high-performance liquid chromatography (HPLC). A reversed-phase HPLC method was developed by optimizing a solvent system for the highly sensitive determination of algal pigments in marine in situ samples. The proposed HPLC system can handle injection volumes grater than 400 mul without a significant loss in the resolution between chlorophyllide a and chlorophyll c_1+c_2. The system also reduces the sample volumes required for the analysis of typical pigments of oligotrophic sea water to 500 ml. Carotenoids characteristic to algal classes such as the Bacillariophyceae, Dinophyceae, Haptophyceae, Cryptophyceae, and Raphidophyceae were analyzed by multiple regression analysis to obtain Chl a contributions of the algal classes to total Chl a. The Chl a contributions of diatoms, raphidophycean flagellates, and dinoflagellates were compared with those calculated from cell counts and observed cell sizes ; there was a good correlation for the estimated algal-classes abundances between pigment and cell count.
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