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Identification and isolation of pyruvate translocator protein of the envelope membranes of C_4 mesophyll chloroplasts.

Research Project

Project/Area Number 03640564
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionSaitama University

Principal Investigator

OHNISHI Jun-ichi  Saitama University, Faculty of Science, Ass. Professor, 理学部, 助教授 (40143242)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsC_4 plants / Sorghum bicolor / Mesophyll cells / Chloroplasts / Envelope membranes / Pyruvate / Translocator / NBD-C1 / NBD-Cl
Research Abstract

A) A comparative study of the plausible mechanism of pyruvate transport into mesophyll chloroplasts, i.e., either Na^+- or H^+- cotransport, was invesitigated in many C_4 species from both dicots and monocots (Gramineae). Light enhancement of pyruvate transport was mimicked in the dark by a H^+-jump only in Andropogeneae and Arundinelleae (12 species) and by a Na^+ jump in all the other species tested (21 species). The result together with our earlier demonstration of Na^+/pyruvate cotransport in a few C_4 species suggests that pyruvate is cotransported either with Na^+ or H^+ depending on C_4 species.
B) The above inferred mechanism of pyruvate transport, H^+/pyruvate cotransport into mesophyll chloroplasts of limited C_4 species was tested by measuring the medium pH change on pyruvate addition in Sorghum and Coix. A good 1 to 1 correlation of the initial rates of pyruvate uptake and H^+ uptake was established, even when pyruvate transport was partially inhibited by NEM or NBD-C1.
C) By incubating Sorghum mesophyll chloroplasts with [^<14>C]NBD-C1 with and without pyruvate or a pyruvate analogue (a cinnamic acid derivative), a peptide band was differentially labelled suggesting the protection from the labelling by substrate. This band was tentatively identified as pyruvate translocator, and is presently being purified.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] N.AOKI: "Two different mechanisms for transport of pyruvate of pyruvate into mesophyllchloroplasts of C_4plants-A comparative study." Plant&Cell Physiology. 33. 805-809 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] J.OHNISHI: "Pyruvate transport into mesophy11 chloroplasts of variousC_4:4plants." Research in Photosynthesis,Vo1.III.Kluwer Academic Publishers.737-740 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] N. Aoki: "Two different mechanisms for transport of pyruvate into mesophyll chloroplasts of C_4 plants - A comparative study." Plant & Cell Physiol.33. 805-809 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] J. Ohnishi: "Pyruvate transport into mesophyll chloroplasts of various C_4 plants. Kluwer Academic Publishers." Research in Photosynthesis, Vol.III, Murata, N. (ed.).737-740 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] N.Aoki: "Two different mechanisms for transport of pyruvate into mesophyll chloroplasts of C_4 plants-A comparative study" Plant & Cell Physiology. 33. 805-809 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] J.Ohnishi: "Transport of pyruvate into mesophyll chloroplasts of various C_4 plants." Research in Photosynthesis.Proceedings of the 9th International Congress on Photosynthesis Nagoya,August-September,1992.

    • Related Report
      1992 Annual Research Report
  • [Publications] J.Ohnishi: "Transport of pyruvate into mesophyll chloroplasts of various C_4 plants." Research in Photosynthesis.Proceedings of the 9th International Congress on Photosynthesis Nagoya,August-September,1992.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] J.Ohnishi,N.Aoki and R.Kanai: "Two different mechanisms of pyruvate transport into C_4 mesophyll chloroplastsーーImplications of comparative study." Plant & Cell Physiology.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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