| Project/Area Number |
03640567
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物生理学
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| Research Institution | Kanazawa University |
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Principal Investigator |
HOSHINA Satoshi Kanazawa University, Faculty of Science Research Associate, 理学部, 助手 (50019486)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Cross-linking / Iron-sulfur center / P700 / Photoinhibition / Photosystem I / Polypeptide / Reaction center / Trypsin / 活性酵素 / クロロフィル / 閃光分光 / ESRスペクトル / 鉄ー硫黄クラスタ- / 熱 / エチレングリコ-ル処理 |
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| Research Abstract |
1. Photosystem I particles (PSI-200) prepared by the solubilization of spinach thylakoids with Triton X-100 have been treated with a cross-linker, dithiobis(succinimidylpropionate) (DSP) which reacts specifically with amino groups. The cross-linking with DSP is concluded to give a simple method for stabilization of the iron-sulfur centers A and B in PSI-200.
2. Irradiation of PSI-200 with high intensity of white light caused a decrease of the electron transport activity from DCIPH_2 to methylviologen and an irreversible bleaching of chlorophyll. The content of P700 was not affected by this treatment. No photoinhibition was, however, shown in PSI complex (Chl/P700 = 40) which was prepared by the solubilization of PSI-200 with Triton X-100, followed by column chromatography on hydroxyl-apatite gel. The column chromatography may give the simple method of PSI preparation for studies on the primary photochemistry.
3. PSI reaction center complex was prepared by the heat-treatment of PSI-200 in the presence of ethylene glycol. The complex was mainly composed with three proteins, PSI-A, PSI-B and PSI-K. The trypsin-treatment of the complex reconstituted into liposomes resulted in the formation of three chlorophyll-protein bands with smaller molecular weight than that of the reaction center complex. Two of them contained P700 and A_1. Characterization of the bands thus obtained is now under investigation.
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