• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on the mitochondrial nucleoids in Euglena

Research Project

Project/Area Number 03640583
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物形態・分類学
Research InstitutionNara Women's University

Principal Investigator

UEDA Katsumi  Nara Women's Uni. Professor, 理学部, 教授 (00031641)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsnucleoid / mitochondria DNA / immunoelectron microscopy / histochemistry / fluorescence microscopy / algae / ヌクレオイド / エチジウムブロミド / クロラムフェニコ-ル / ユ-グレナ / 細胞小器官
Research Abstract

(1) Concerning the effects of ethidium bromide and chloramphenicol on the mitochondrial nucleoids.
Ethidium bromide (EB) stopped the multiplication of cells and decreased their respiratory activity by 55% after treatment for 10 days. Most of the mitochondria became slender with few cristae and some became cup-shaped with stacked cristae. Mitochondrial nucleoids decreased markedly in number after treatment with EB for more than 2 days. After treatment for 3 days with EB, mitochondrial nucleoids could not be detected in about half of all cells examined. Treatment with CAP for 10 days reduced the respiratory activity by 47%. CAP did not decrease the number of mitochondrial nucleoids but it increased the number of cristae and the volume of mitochondria.
(2) Concerning the detection of DNA in the nucleoids of chloroplasts and mitochondria by immunoelectron microscopy.
Nucleoids in chloroplasts appeared to be composed of twisted threads of 50-70nm in diameter. The twisted threads were entangled to form thicker nodes of 100-200nm in diameter. Most nucleoids in mitochondria were spherical or ovoid, 70-130nm in diameter. Nucleoids both in chloroplasts and in mitochondria contained cores with which DNA threads were in tight contact. These apparent structures of nucleoids were very different from those previously observed by conventional electron microscopy.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] CHIDA,Y.and UEDA,K.: "Changes in the lysosome structure during the formation of zoospores in Trebouxia potteri." Protoplasma. 171. 19-27 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] CHIDA,Y.and UEDA,K.: "Detection of actin on organelles of Trebouxia potteri,in particular on the surface of lysosomes." Protoplasma. 171. 28-33 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] HAYASHI,Y.and UEDA,K.: "Effects of ethidium bromide and chloramphenicol on mitochondrial nucleoids in Euglena gracilis." Physiologia Plantarum. 86. 57-62 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] UEDA,K.and NONAKA,M.: "Division of chloroplasts in a green alga,Pedias trum duplex." Annales of Botany. 69. 113-118 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] CHIDA,Y.and UEDA,K.: "Division of chloroplasts in a green alga,Trebouxia potteri." Annales of Botany 67. 67. 435-442 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Chida, Y. and Ueda, K.: "Changes in the lysosome structure during the formation of zoospores in Trebouxia potteri." Protoplasma. 171. 19-27 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Chida, Y. and Ueda, K.: "Detection of actin on organelles of Trebouxia potteri, in particular on the surface of lysosomes." Protoplasma. 171. 28-33 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hayashi, Y. and Ueda, K.: "Effects of ethidium bromide and chloramphenicol on the mitochondrial nucleoids in Euglena gracilis." Physiol. Plant.86. 57-62 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ueda, K. and Nonaka, M.: "Division of chloroplasts in a green alga, Pediastrum duplex." Ann. Bot.69. 113-118 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Chida, Y. and Ueda, K.: "Division of chloroplasts in a green alga, Trebouxia potteri." Ann. Bot.67. 435-442 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Chida,Y.and Ueda,K.: "Changes in the lysosome structure during the formation of zoospores in Trebouxia potteri." Protoplasma. 171. 19-27 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Chida,Y.and Ueda,K.: "Detection of actin on organelles of Trebouxia po tteri,in particular on the surface of lysosomes." Protoplasma. 171. 28-33 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hayashi,Y.and Ueda,K.: "Effects of ethidium bromide and chloramphenicol on mitochondrial nucleoids in Euglena gracilis." Physiologia Plantarum. 86. 57-62 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ueda,K.and Nonaka,M.: "Division of chloroplasts in a green alga,Pedias trum duplex." Annales of Botany. 69. 113-118 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Chida,Y.and Ueda,K.: "Division of chloroplasts in a green alga,Trebouxia potteri." Annales of Botany 67. 67. 435-442 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] HAYASHI,Y.and Ueda,K.: "Effects of ethidium bromide and chloramphenicol on the mitochondrial nucleoids in Euglena gracilis" Physiologia Plantarum. (1992)

    • Related Report
      1991 Annual Research Report

URL: 

Published: 1991-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi