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Detection of Germ Cell Line in Marine Invertebrate

Research Project

Project/Area Number 03640604
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionOkayama University

Principal Investigator

YAMAMOTO Masamichi  Fac.Sci., Okayama Univ.Prof., 理学部, 教授 (50011565)

Co-Investigator(Kenkyū-buntansha) SHIRAI Hiroko  Fac.Sci., Okayama Univ.Assoc.Prof., 理学部, 助教授 (50090478)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Keywordscephalopod / ascidian / starfish / germ cell / gonad / meiosis / ovary / testis / 減数分裂 / 始原生殖細胞 / ヤムシ / ユ-レイボヤ / 生殖巣 / 微細構造 / 幼生 / 変態
Research Abstract

1. The origin of the germ cell and the early processes of the gonad formation were clarified in a octopus, Octopus ocellatus. The earlygerm cells apear in the pericardial cavity. In the female, the germ cells begin meiosis just before hatching while in the females the germ cells enter into meiosis 7 weeks after hatching.
2. The origin of the germ cell and the process of sex differentiation of the gonads were elucidated in a solitary ascidian, Ciona intestinalis. The germ cells first appear in the juvenile just after metamorphosis in contact with the degenerating tissue mass which arises near the esophagus. The form a spherical body (early gonad), which divides into the testis and the ovary during migration along the wall of the stomach to the pyloric portion.
3. The origin of the germ cell and the process of gonad formation became clear in a star fish, Asterina pectinifera. The germ cells, about 10 in number, originate in the third coelomic pouch of the brachyolaria larvae. The enter into the haemal system and form the gonads after metamorphosis.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] M.A.B.Catalan: "The effect of pH on meiosis reinitiation in oocyte of the prosobranch mollusc,Ccttana nigrolineata." Invert.Repro.Dev.23. 211-213 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.A.B.Catalan: "Annual reproductive cycle of the prosobranch limpet,cellana nigrolineata." Invert.Repro.Dev.24. 127-136 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] C.Inoue: "germ cell differentiation in starfish:The posterior entero coel as the origin of germ cells." Develop.Growth Differ. 34. 413-418 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Kiyomoto: "Reconstruction of starfish eggs by electric fusion" Develop.Growth Differ.35. 107-114 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Kiyomoto: "The determination for archenteron formation in starfish." Develop.Growth Differ.35. 99-105 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Takenaka: "Remodeling of the nauplius eye into adult oolli during metamorphosis of the barnacle." Develop.Growth Differ. 35. 245-255 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Okada: "Identification of early oogenetic cells in the solitary ascidians." Develop.Growth Differ.35. 495-506 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Katayama: "phylogenetic position of acoel turbellarians inferred from partial 18S rDNA sequence" Zool.Sci.10. 536 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Sakairi: "Possible MIS production by follicle cells in spontaneous oocyte maturation of the ascidion." Develop.Growth Differ. 33. 155-162 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] C.Inoue: "Origin of germ cells and early differentiation of gonads in starfish." Develop.Growth Differ. 33. 217-226 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.A.B.Catalan: "Annual reproductive cycle of two Japanese species of Sipunculans:" Pacific Science. 48. 145-157 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Sakairi & Shirai: "Possible MIS production by follicle cells in spontaneous oocyte maturation of the ascidian." Develop, Growth Differ.33. 155-162 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] C.Inoue & H.Shirai: "Origin of germ cells and early differentiation of gonads in the starfish." Develop, Growth Differ.33. 217-226 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] C.Inoue, M.Kiyomoto & H.Shirai: "Germ cell differentiation in starfish : The posterior enterocoel as the origin of germ cells." Develop, Growth Differ.34. 413-418 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Katayama, M.Yamamoto et al.: "Phylogenetic position of acoel turbellarians inferred from partial 18SrDNA sequence" Zool.Sci.10. 529-536 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Okada & M.Yamamoto: "Identification of early oogenetic cells in the solitary ascidians Ciona savignyi and Ciona intestinalis." Develop.Growth Differ.35. 495-506 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Takenaka, M.Yamamoto et al.: "Remodeling of the naulpius eye into the adult ocelli during metamorphosis of the barnacle." Develop.Ggowth Differ.35. 245-255 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Kiyomoto & H.Shirai: "The determinant for archenteron formation in starfish : Co-culture of an animal egg fragment-derived call cluster" Develop.Growth Differ.35. 89-98 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Kiyomoto & H.Shirai: "Reconstruction of starfish eggs by electric cell fusion : a new method to detect the cytoplasmic determinant." Develop.Growth Differ.35. 99-106 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Catalan & M.Yamamoto: "The effect of pH on meiosis reinitiation in oocytes of the prosobranch mollusc Cellana nigrolineata." Invert.Repro.Dev.23. 211-213 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Catalan & M.Yamamoto: "The annual reproductive cycle of the prosobranch limpet Cellana nigrolineata." Invert.Repro.Dev.24. 127-136 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Catalan & M.Yamamoto: "Annual reproductive cycle of two Japanese species of Sipunculans : Siphonosoma cumanense and Phascolosoma scolops." Pacific Sci.48. 145-157 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Okada: "Identification of Early Oogenetic cells in the Solitary Ascidians,Ciona Savignyi and ciona intestinalis." Develop.Growth Differ.35. 495-506 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Takenaka: "Remodeling of the nauplius eye into adult ocelli during metamorphosis of the barnacle,Balamus amphitrite hawaiiensis." Develop.Growth Differ.35. 245-255 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Katayama: "Phylogenetic position of acoel turbellarians interred from partial 18S γDNA sequences" Zool.Sci. 10. 529-536 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.A.B.Catalan: "The effect of pH on meiosis reinitiation in oocyte of the prosobranch mollusca cellana nigrolineata(Reeves)." Invert.Repro.Dev.23. 211-213 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.A.B.Catalan: "Annual reproductive cycle of the prosobranch limpet,cellana nigrolineata(Reeves)." Invert.Repro.Dev.24. 127-136 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] C.Inoue: "Germ cell differantiation in starfish:The posterion enterocoel as the origin of germ cells in Asterina pectinitera" Develop.Growth Differ. 34. 413-418 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Kiyomoto: "The determination for archenteron formation in starfish" Develop.Growth Differ. 35. 99-105 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Kiyomoto: "Reconstruction of starfish eggs by electric cell fusion" Develop Growth Differ. 35. 107-114 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T. Hara et.al.: "Localization of retinochrome in the retina of a tetrabranchiate cephalopod." Zoological Science. 9. 211-217 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] A.B.Catalan M.Yamamoto: "The annual reproductive cycles in two species of the Sipuncula." Contributions Ushimado Marine Laboratory. 30. 1-9 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] C.Inoue, M.Kiyomo H.Shirai: "Germ cell differentiation in star fish" Develop. Growth Differ.34. 413-418 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Sakairi: "Possible MIS Production by Follicle Cells in Spontaneous Ooayte Maturation of the Ascidian,Halocynthia roretzi." Develop.Growth Ditler.33. 155-162 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] C.Inoue: "Origin of Germ Cells and Early Differentiation of Gonads in the Star fish,Asterina pectinifera." Develop.Growth Differ.33. 217-226 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Okada: "Immuno electronmicroscopic Study of germ cells in Ciona savignyi" Zool.Sci.8. 1067 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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