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Primary structure of the myoglobins from the invertbrate and lower vertebrate and its molecular evolution.

Research Project

Project/Area Number 03640607
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionKochi University

Principal Investigator

FURUKOHRI Takahiro  Kochi Univ., Fac.Sci., Professor, 理学部, 教授 (30036553)

Co-Investigator(Kenkyū-buntansha) SUZUKI Tomohiko  Kochi Univ., Fac.Sci., Assistant Professor, 理学部, 助教授 (60145109)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsMyoglobin / Hemoglobin / Mollusca / Amino Acid Sequence / Phylogenetic Tree / Molecular Evolution / Liolophura / barbatia
Research Abstract

Globins from the chiton, Liolophura japonica and blood clam, Barbatia virescens were investigated.
1. The blood clam B.virescens has a heterodimeric hemoglobin in erythrocytes. The complete amino acid sequence of chain I of B.virescens has been determined. The sequence was mainly determined from CNBr-peptides and their subpeptides, an the alignment of the peptides was confirmed by sequencing of PCR-amplified cDNA for B.virescens chain I.The cDNA-derived amino acid sequence matched completely with the sequence proposed from protein sequencing. An evolutionary tree for Anadara and Barbatia chains clearly indicates that all of the chains are evolved from one ancestral glolin gene, and that the divergence of chains has occurred in each clam after the speciation. The evolutionary rate for clam hemoglobins was estimated to be about four times faster than that of vertebrate hemoglobin. We suggest that blood clam hemoglobin is a physiologically less important molecule when compared with vertebr … More ate hemoglobins, and so it evolved rapidly and resulted in a remarkable diversity in quaternary and subunit structure within a relatively short period.
2. Myoglobin was isolated from the radular muscle of the chiton, L.japonica, a primitive archigastropodic mollusc. Liolophura contains three monomeric myoglobins(I,II,III), and the complete amino acid sequence of myoglobin I has been determined. The E7 distal histidine is conserved in Liolophura myoglobin. The autoxidation rate at physiological conditions indicated that Liolophura oxymyoglobin is fairly stable when compared with other molluscan myoglobins, A phylogenetic tree was constructed from 19 molluscan globin sequences. Liolophura myoglobin was placed on the branch of monomeric myoglobin lineage, showing that it diverged earlier from other monomeric myoglobins. The hemoglobin cluster is also divided into two subclusters. One cluster contains homodimeric, heterodimeric, tetrameric, and didomain chains of erythrocyte hemoglobins of the blood clam. Other cluster consists of three hemoglobin chains derived from the bacterial symbiont-harboring clams Calyptogena and Lucina, in which hemoglobins are suppossed to play an important role in maintaining the symbiosis withsulfide bacteria. Less

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 鈴木知彦: "Primary structure of chain I of the heterodimenic hemoglobin from the blood clam Barbatia virescens." J.Protein Chemistry.11. 629-633 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 鈴木知彦: "Amino acid sequence of myoglobin from the chiton Liolophura japonica and a phylogenetic tree for molluscan globins." J.Protein Chemistry.12. 45-50 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Suzuki, Tomohiko: "Primary structure of chain I of the heterodimeric hemoglobin from the blood clam Barbatia virescence" J.Protein Chem.11(6). 629-633 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Suzuki, Tomohiko: "Amino acid sequence of myoglobin from the chiton Liolophura japonica and a phylogenetic tree for molluscan globins." J.Protein Chem.12(1). 45-50 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 鈴木知彦: "Amino acid sequence of myoglob-in from the Chiton Liolophura japonicaand a phylogcnetic tree for Molluscanglobins." Jouvnal of Protein Chemistry. 12. 45-50 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 鈴木 知彦: "Primary Structure of Chain I of the Heterodimeric Hemoglobin from the Blood clam Barbatia virescens." Jouvnal of Protein Chemistry. 11. 629-633 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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