| Project/Area Number |
03640614
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | Keio University |
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Principal Investigator |
MATSUMOTO Jiro Keio University, Law and Political Science, Professor, 法学部, 教授 (80051241)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZAKI Katsumi Keio University, Law and Political Science, Assistant, 法学部, 嘱託
宮崎 勝巳 慶應義塾大学, 法学部, 嘱託
広瀬 裕一 慶応義塾大学, 法学部, 嘱託
秋山 豊子 慶應義塾大学, 法学部, 助教授 (30118898)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Transgenic fish / Orange-colored medaka / Tyrosinase gene / Melanogenesis / Melanophore / Melanosome / Mendelian law / Electroporation / メラニン形成 / トラススジェニックメダカ / チロシナ-ゼ遺伝子 / メラノフォア(黒色素胞) / メラノソ-ム |
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| Research Abstract |
Transgenic fish bearing the gene for mouse tyrosinase are produced by microinjecting it into the oocyte nucleus of or by introducing it by electroporation into the fertilized egg of an orange-colored variant of medaka (Oryzias latipes). We successfully obtain 41 transgenic fish exhibiting brown skin pigmentation, 3 fish by the former method and 38 by the latter. Brown skin pigmentation in these transgenic fish is found to result from melanogenesis in "amelanotic melanophores", which are distributing in an orange-colored variant of this species. immunofluorescence using the anti-mouse tyrosinase antibody discloses and apparent distribution of mouse-type tyrosinase in these melanophores. Electron microscopy on the skin of transgenic fish discloses that the melanophores are installed with numerous, unevenly sized melanosomes having a lattice-like internal structure, which is different from "multivesicular" ones common to fish melanophores, and that little melanin deposition is detectable in xanthophores and leucophores. All these findings strongly suggest that the gene transfected is expressed under a cell-type specific manner in medaka. Crossing between a transgenic fish and a fish from an orange-colored variant yields offsprings having wild-type brown and orange-colored pigmentation at an approximate rate of 1 : 1, suggesting transmission of the transfected gene according to the Mendelian law.
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