• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation on synaptic architecture of insect deutocerebrum with intracellular staining and imunoelectron microscopical methods.

Research Project

Project/Area Number 03640619
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionFUKUOKA UNIVERSITY

Principal Investigator

TOMINAGA Yoshiya  FUKUOKA UNIV., BIOLOGICAL LAB., Professor, 理学部, 教授 (70078591)

Co-Investigator(Kenkyū-buntansha) SHIMOHIGASHI Miki  FUKUOKA UNIV., BIOLOGICAL LAB., Research Associate, 理学部, 助手 (60078590)
NISHIKAWA Michiko  FUKUOKA UNIV., BIOLOGICAL LAB., Research Associate, 理学部, 助手 (30078563)
ITO Tunao  FUKUOKA UNIV., BIOLOGICAL LAB., Lecturer, 理学部, 講師 (40131809)
YOKOHARI Fumio  FUKUOKA UNIV., BIOLOGICAL LAB., Professor, 理学部, 教授 (20117287)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsHygroreception / Thermoreception / Antennal lobe / Interneuron / CNS / Deutocelebrum / Glomerulus / Macro-glomerulus complex / 湿度受容 / 温度受容 / 介在神経繊維 / 触角葉 / 前大脳 / 電気生理
Research Abstract

Hygro- and thermoreception is closely related to survival of animals, and particularly so in insects. As the ratio of surface area to body size is large, much water evaporates from the body surface and body temperature is much affected by their environment. Therefore hygro- and thermoreception is as important for life as other sensory reception. The hygro- and thermoreceptors are present on antennae of insects. It is well known that most and probably all axons of antennal olfactory receptor cells in the third segment, the flagellum, have terminal arborizations in the antennal lobe. The antennal lobe comprises two types of glomeruli, ordinary glomeruli (OGs) and a macro-glomerulus complex (MGC) in male. Axons of pheromone receptor cells terminate in MGC and other axons of olfactory cells terminate in OGs. However, there were no reliable data about the terminations of axons of the hygro- and thermoreceptors and olfactory receptors other than specific receptors such as pheromone receptors … More . We stained electrophoretically the axons of these receptors and an enanthic acid receptor (olfactory receptor) from the sensilla on Periplaneta antennae. We have found that the axons of the hygro- and thermoreceptors have terminal arborizations in OG specific to each type of receptors. Furthermore, the axon of the enanthic acid receptor also teminates in other specific OG. These results suggest that each receptor axon terminates in specific glomerulus, i.e. sensory information is simultaneously processed in the glomerulus specific to each receptor. Besides primary afferents, there are three types of interneurons in the antennal lobe : local interneurons (LNs), projection neurons (PNs) and centrifugal neurons. The sensory information flows from LNs to PNs in a traditional working hypothesis. However, some LNs reacted to humidity or temperature change with a long delay after stimulations and some PNs reacted quickly in our experiment. This suggests that such PNs are directly connected to some axons of hygro- and thermal receptor cells. Less

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] 西川 道子ら(西川 道子): "Response characteristics of two types of cold receptors on the antenna of Periplaneta americana." Journal of Compartive Physiology A. 171. 299-307 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 伊東 綱男ら(伊東 綱男): "External Morphology of sensilla in the sacculus of an antennal flagellum of the fruit fly,Drosophila melanogaster Meigen(Diptera,Drosophilidae)." International Journal of Insect Morphology and Embryology. 20. 235-243 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] D.Blaustein ら (D.Blaustein): "Ultrastructural localization of odorant(AMP)binding sites on the dendrites of olfactory receptor cells of the spiny lobster." Chemical Sense.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 伊東 綱男ら(伊東 綱男): "Responses to humidity change of deutocerebral interneurons of the honeybee,Apis mellifera L." Naturwissenschaften. 78. 320-322 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 西川 道子ら(西川 道子): "Deutocerebral interneurons responding to thermal stimulation on the antennae of the cockroach,Periplaneta americana L." Naturwissenschaften. 78. 563-565 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 藤村 幸一ら(藤村 幸一): "Classification of antennal olfactory receptor of the cockroach,Periplaneta americana." Zoological Science. 8. 243-255 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 横張 文男ら(分担執筆)(横張 文男): "Primary process of hygroreception and humidity information processing in brain of insects.In:Perspectives in Neuroethology pp.101-114" Kyoto Univ.Press, 160 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Ito, F,Yokohari and Y.Tominaga: "Response to humidity change of deutocerebrum of the honeybee, Apis mellifera L" Naturwissenschaften. 78. 320-322 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Nishikawa, F.Yokohari and T.Ishibashi: "Deutocerebral interneurons reponding to thermal sitmulation on the antennae of the cockroach, Periplaneta americana L" Naturwissenschaften. 78. 563-565 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Itoh, F.Yokohari, T.Tanimura and Y.Tominaga: "External morphology of sensilla in the sacculus of an antennal flagellum of the fruit fly Drosophila melanogaster Meigen (Diptera : Drosophilidae)" Int.J.Insect Morphol. & Embryol. 20. 235-243 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Fujimura, F.Yokohari and H.Tateda: "Classification of antennal olfactory receptors of the cockroach, Periplaneta americana L" Zoological Science. 8. 243-255 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Nishikawa, F.Yokohari and T.Ishibashi: "Response characteristics of two types oJ.f cold receptors on the antennae of the cockroach, Periplaneta americana L" J.Comp. Physiol.A. 171. 299-307 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] D.N.Blaustein, R.B.Simmons, M.F.Burgess, C.D.Derby, M.Nishikawa and K.S.Olson: "Ultrastructural locarization of 5' AMP odorant receptor sites on the dendrites of olfactory receptor neurons of the spiny lobster" Chemical Sense.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] F.Yokohari, T.Itoh and M.Nishikawa: "Primary process of hygroreception and humidity information processing in brains of insects" In : Perspectives in neuroethology Kyoto univ.Press. 101-114 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 西川 道子 ら: "Response characteristics of two types of cold receptors on the antenna of Periplaneta americana." Journal of Compartive Physiology A. 171. 299-307 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] D.Blaustein ら: "Ultrastructural localization of odorant(AMP)binding sites on the dendrites of olfactory receptor cells of the spiny lobster." Chemical Sense. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 伊東 綱男 ら: "Humidity-responsive interneurons in the deutocerebrum of the honeybee" 3rd International Congress of Comp.Physiol.and Biochem.(abstract). 126- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 西川 道子 ら: "Central projection of antennal thermoreceptor axons and thermo-responsive interneurons in deutocerebrum of cockroach." 3rd International Congress of Comp.Physiol.and Biochem.(abstract). 165- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 下東 美樹 ら: "Spectral receptor types in the compound eye and their projection to optic neuropiles in the cabbage butterfly." 3rd International Congress of Comp.Physiol.and Biochem. (abstract). 148- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 横張 文男 ら(分担執筆): "Primary Process of hygroreception and humidity information processing in brain of insects.In:Perspectives in Neuroethology pp.101-114" Kyoto Univ.Press, 160 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Fujimura,K.,Yokohari,F.& Tateda,H.: "Classification of antennal olfactory receptors of the cockroach,Periplaneta americana L." Zoological Science. 8. 243-255 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Itoh,T.,Yokohari,F.,Tanimura,T.& Tominaga,Y.: "External Morphology of sensilla in the sacculus of an antennal flagellum of the fruit fly Drosophila melanogaster Meigen (Diptera:Drosophilidae)." Int.J.Insect Morphol.& Embryol.20. 235-243 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Itoh,T.,Yokohari,F.& Tominaga,Y.: "Response to humidity change of deutocerebral interneurons of the honeybee,Apis mellifera L." Naturwissenschaften. 78. 320-322 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nishikawa,M.,Yokohari,F.& Ishibashi,T.: "Deutocerebral interneurons responding to thermal stimulation on the antennae of the cockroach,Periplanata americana L." Naturwissenschaften. 78. 563-565 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shimohigashi,M. Tominaga,Y.: "Identification of UV,green and red receptors,and their projection to Iamina in the cabbage butterfly,Pieris rapae." Cell Tissue Res.263. 49-59 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nishikawa,M.,Yokohari,F.& Ishibashi,T.: "Response characteristics of two types of cold receptors on the antennae of the cockroach,Periplanata americana L." J.Comp Physiol.A. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yokohari,F.,Itoh,T.& Nishikawa,M.: "Animal behavior and neuroethology Ed.K.Kubota" Kyoto Univ.Press, (1992)

    • Related Report
      1991 Annual Research Report

URL: 

Published: 1991-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi