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Research on the primitive complement system in agnathan cyclostomes

Research Project

Project/Area Number 03640631
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物形態・分類学
Research InstitutionHiroshima Women's University

Principal Investigator

FUJII Tamotsu  Hiroshima Women's University, Faculty of Home Economics, Associate Professor, 家政学部, 助教授 (10181314)

Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsCyclostome / Hagfish / Primitive complement system / Alternative pathway / Third component of complement (C3) / Irregular cleavage of pro-C3 / Opsonin / アイソタイプ / 代替(第2)経路 / 補体第3成分
Research Abstract

A protein from hagfish serum that cross-reacted with the third component of hagfish complement (C3-1) was purified to homogeneity and its structural properties were compared with those of two-subunit-chain (115 kDa and 72 kDa) C3-1. This protein (termed C3-2), when purified from the plasma, consisted of three disulfide-linked polypeptide chains (77 kDa, 72 kDa and 30kDa). On immunoelectrophoresis, purified C3-2 had a more anodal mobility compared to the beta mobility of C3-1. However, immunochmical analysis revealed that C3-2 after the initial step in the purification consisted of two disulfide-linked polypeptide chains (105 kDa and 72 kDa). Treatment of C3-2 with methylamine, prior to the spectrophotometric titration of free SH content in this molecule, did not significantly affect the titration end point, suggesting the disruption of a thiol ester bond in C3-2. Amino acid sequence analysis of the amino-termini of the subunits of C3-2 revealed that 77 amino acid residues at the amino-terminus of native alpha-chain were missing from the 77-kDa as well as 105-kDa polypeptides from C3-2. These results indicate that C3-2 isolated in this study is analogous to C3b. Furthermore, sequencing data indicate that most of the native C3 from hagfish occur in the serum with an irregular two-subunit (alpha + gamma and beta) chain structure, as a result of the one-sided processing of putative hagfish pro-C3 at the beta-alpha processing site exclusively, and, moreover that only the molecular features of degenerated hagfish C3 (C3-2) are altered during its purification to generate a three-chain structure

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 藤井 保: "補体系の系統発生" 細胞. 23. 543-547 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 藤井 保: "円口類の生体防御機構" 臨床免疫. 23. 1625-1635 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujii,T.: "Isolation and characterization of a protein from hagfish serum that is homologous to the third component of the mammalian complement system." Journal of Immunology. 148. 117-123 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Holland,N.D.: "Engrailed expression during development of a lamprey,Lampetra japonica:a possible clue to homologies between agnathan and gnathostome muscles of the mandibular arch." Development,Growth & Differentiation. 35. 153-160 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujii,T.: "Unique structure of hagfish complement C3:Occurrence of immature C3 as a result of irregular cleavage of pro-C3." Journal of Immunology. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujii, T.: "Phylogeny of complement system (in Japanese)" Saibou. 23. 543-547 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujii, T.: "Host-defense mechanisms in cyclostomes (in Japanese)" Rinshou Men-eki. 23. 1625-1634 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujii, T., T.Nakamura, A.Sekizawa, and S.Tomonaga: "Isolation and characterization of a protein from hagfish serum that is homologous to the third component of the mammalian complement system" Journal of Immunology. 148. 117-123 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Holland, N.D., L.Z.Holland, Y.Honma, and T.Fujii: "Engrailed expression during development of a lamprey, Lampetra japonica : a possible clue to homologies between agnathan and gnathostome muscles of the mandibular arch" Development, Growth and Differentiation. 35. 153-160 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujii, T., T.Nakamura, and S.Tomonaga: "Unique structure hagfish complement component C3 : Ocuurence of immature C3 as a rusult of irregular cleavage of pro-C3" Submitted for publication to Journal of Immunology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Holland, N.D., L.Z. Holland,Y. Honma, and T. Fujii: "Engrailed expression during development of a lamprey, Lampetra japonica: a possible clue to homologies between agnathan and gnathostome muscles of mandibular arch." Development, Growth and Differentiation. 35. 153-160 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fujii, T., T. Nakamura, S. Tomonaga: "Identification and characterization of a variant of the third component of complement (C3) in hagfish serum." Zoological Science. 10, (Supplement). 83 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fujii, T., S. Tomonaga, R.Fujii, and A. Sekizawa: "Characterization of opsonins occurring in normal hagfish serum." Developmental and Comparative Immunology. 18, (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fujii,T.,T.Nakamura A.Sekizawa and S.Tomonaga: "Isolation and characterization of a protein from hagfish serum that is homologous to the third component of the mammalian complement system." Journal of Immunology. 148. 117-123 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 藤井 保: "円口類の生体防御機構" 臨床免疫. 23. 1625-1634 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 藤井 保: "補体系の系統発生" 細胞. 23. 543-547 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Jujii,T.,T.Nakamura,A.Sekizawa and S.Tomonaga: "Isolation and characterization of a protein from hagfish serum that is homologous to the third component of the mammalian complement system" Journal of Immunology. 148. 117-123 (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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