| Project/Area Number |
03640637
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物形態・分類学
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| Research Institution | The Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
SAKAIZUMI Mitsuru The Tokyo Metropolitan Institute of Medical Science, Department of Laboratory Animal Science, Researcher, 実験動物研究部門, 研究員 (40175360)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Oryzias latipes / medaka / mitochondrial DNA / RFLP / sequence data / 12srDNA / cytochrome b / ND4 / D-1oop / 制限酵素 |
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| Research Abstract |
Oryzias latipes includes four genetically distinct groups, the Northern population, the Southern population, the East Korean population and the china-West Korean population on the basis of electrophoretic data.
The Northern population and the Southern population were distinguished by RFLP (using eight 6-base enzymes) of mitochondrial DNA. The former included 10 haplotypes that were composed of a few groups closely related to each other. The latter included 89 haplotypes that could be divided into several groups with a lower level of difference. Sequence divergence was estimated to be 10.1% between a "northern" haplotype (haplotype 1) and a "southern" haplotype (haplotype 22).
This value was thought to be very high as those between intraspecific haplotypes.
We made two libraries of wkole mitochondrial DNA from a "northern" haplotype (1) and a "southern" haplotype (32). Three different regions encoding 12srRNA, cyt. b and ND4 were sequenced. The sequence differences between "northern" and " southern" haplotypes were 3.9% (15/586) in 12srDNA, 11.6% (64/554) in cyt. b and 9.9% (22/223) in ND4 region. Although the values differed among different regions, sequence divergence values were close to the estimate (10.1%) from RFLP analyses.
These results indicated that the Northern population and the Southern population are highly differentiated from a view point of molecular phylogeny.
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