• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Structural Elucidation of Photosynthetic antenna and Its Application for Light Harvesting System.

Research Project

Project/Area Number 03650638
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 工業物理化学・複合材料
Research InstitutionTohoku University

Principal Investigator

NOZAWA Tsunenori  Tohoku Univ., Dept. Biochem. & Engin., Professor, 工学部, 教授 (10006322)

Co-Investigator(Kenkyū-buntansha) MORISHITA Yumi  Tohoku Univ., Dept. Biochem. & Engin., Research Associate, 工学部, 助手 (70239648)
OSAWA Masatoshi  Tohoku Univ.,Dept. Mol. Chem. & Engin., Associate Professor, 工学部, 助教授 (00108466)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsThermostability / Photosynthetic Bacteria / Photosynthetic Antenna / CP / MAS^<13>C NMR / Chlorophyll / Excitation Energy Transfer / FTIR / Time Resolved Fluorescence / NMR / 光合成色素 / ラマン / IR / 会合構造 / CP / MAS NMR
Research Abstract

We have succeeded in preparing photosynthetic antennas from photosynthetic membranes of Chloroflexus aurantiacus and Chlorobium tepidum. The isolated antenna chlorosomes were studied by a solid state high resolution (cross polarization / magic angle spinning (CP/MAS)) ^<13>CNMR. We have clarified the cooridation states of the bacteriochlorophyll (BChl) c's, the interactions between BChl c's and the interactions of BChl c's with surrounding amino acid residues in native states and in situ. We have purified various BChl c and d derivatives by extraction with organic solvent and HPLC. The structures of the pigments were determined with one and two dimensional ^1H and ^<13>C NMR and FTIR. Excitation energy transfers in the antenna systems and also between the antennas and reaction centers were investigated with a pico-second time resolved fluorescence spectrometry. The results gave an evidence to elucidate the structures and mechanisms for the antenna systems to collect and utilize the light energy. The model antenna systems which resembled the native chlorosome antenna have been prepared for the first time from the purified BChl c and d derivatives with clarified structures. The organization of the synthesized model antenna were investigated with CP/MAS^<13>C NMR and FTIR. These investigations offered a guide line to construct a model antenna system and to utilize light energy efficiently.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] T.NOZAWA,M.SUZUKI,S.KANNO and S.SHIRAI: "CP/MAS^<13>CNMR Studies on the Structure of Bacteriochlorophyll c in Chlorosomes from Chloroflexus aurantiacus" Chem.Lett. 1805-1808 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.NOZAWA,T.NOGUCHI and M.TASUMI: "Resonance Raman Studies on the Structure of Bacteriochlorophyll c in Chlorosomes from Chloroflexus aurantiacus" J.Biochem.108. 737-740 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.NOZAWA,M.SUZUKI,K.OHTOMO,Y.MORISHITA,H.KONAMI and Michael T.Madigan: "Aggregation Structure of Bacteriochlorophyll c in Chlorosomes from Chlorobium tepidum" Chem.Lett.1641-1644 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.NOZAWA,K.OHTOMO,M.SUZUKI,Y.MORISHITA,H.KONAMI and Michael T.Madigan: "Structures of Bacteriochlotrophyll c′s in Chlorosomes from a New Thermophilic Bacterium Chlorobium tepidum" Chem.Lett.1763-1766 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.NOZAWA,K.OHTOMO,N.TAKESHITA,Y.MORISHITA,M.OHSAWA and Michael T.Madigan: "Substituent Effects on the Aggregation of Bacterochlorophyll d Homologues Purified from Chlorobium limicola" Bull.Chem.Soc.Jpn.65. 3493-3494 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.NOZAWA,K.OHTOMO,Y.MORISHITA,H.KONAMI and Michael T.Madigan: "Dimer Structures of Bacteriochlorophyll c from Chlorobium tepidum in CDCl_3" Chem.Lett. 261-264 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.NOZAWA,K.OHTOMO,M.SUZUKI,Y.MORISHITA and Michael T.Madigan: "Structures and Organization of Bacteriochlorophyll c′s in Chlorosomes from a New Thermophilic Chlorobium tepidum" Bull.Chem.Soc.Jpn.66. 1-7 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Nozawa,K.Ohtomo,M.Suzuki,Y.Morishita and H.Konami: "Progress in Photosynthesis Research C/MAS ^<13>CNMR Studies on Antenna Structures in Chlorosomes in Green Bacteria" Kluwer Academic Publishers, 4 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Nozawa, M.Suzuki, S.Kanno and S.Shirai: "CP/MAS^<13>C NMR Studies on the Structure of Bacteriochlorophyllc in Chlorosomes from Chloroflexus aurantiacus" Chem. Lett.1805-1808 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Nozawa, T.Noguti and M.Tasumi: "Resonance Raman Studies on the Structure of Bacteriochlorophyll c in Chlorosomes from Chloroflexus aurantiacus" J. Biochem.108. 737-740 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Nozawa, M.Suzuki, K.Ohtomo, Y.Morishita, H.Konami and Michael T.Madigan: "Aggregation Structure of Bacteriochlorophyll c in Chlorosomes from Chlorobium tepidum" Chem. Lett.1641-1644 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Nozawa, K.Ohtomo, M.Suzuki, Y.Morishita, H.Konami and Michael T.Madigan: "Structures of Bacteriochlorophyll c's in Chlorosomes from a New Thermophilic Bacterium Chlorobium tepidum" Chem. Lett.1763-1766 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Nozawa, K.Ohtomo, N.Takeshita, Y.Morishita, M.Ohsawa and Michael T.Madigan: "Substituent Effects on the Aggregation of Bacteriochlorophyll d Homologues Purified from Chlorobium limicola" Bull. Chem. Soc. Jpn.65. 3493-3494 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Nozawa, K.Ohtomo, Y.Morishita, H.Konami and Michael T.Madigan: "Dimer Structures of Bacteriochlorophyll c from Chlorobium tepidum in CDCl_3" Chem. Lett.261-264 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Nozawa, K.Ohtomo, M.Suzuki, Y.Morishita and Michael T.Madigan: "Structures and Organization of Bacteriochlorophyll c's in Chlorosomes from a New Thermophilic Bacterium Chlorobium tepidum" Bull. Chem. Soc. Jpn.66. 1-7 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Nozawa, K.Ohtomo, M.Suzuki, Y.Morishita and H.Konami: "CP/MAS^<13>C NMR Studies on Antenna Structures in Chlorosomes in Green Bacteria" Progress in Photosynthesis Research. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Nozawa,M.Suzuki,S.Kanno and S.Shirai: "CP/MAS^<13>CNMR Studies on the Structure of Bacteriochlorophyll c in Chlorosomes from Chloroflexus aurantiacus" Chem.Lett. 1805-1808 (1990)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Nozawa,T.Noguti and M.Tasumi: "Resonance Raman Studies on the Structure of Bacteriochlorophyll c in Chlorosomes from Chloroflexus aurantiacus" J.Biochem.108. 737-740 (1990)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Nozawa,M.Suzuki,K.Ohtomo,Y.Morishita,H.Konami and Michael T.Madigan: "Aggregation Structure of Bacteriochlorophyll c in Chlorosomes from Chlorobium tepidum" Chem.Lett.1641-1644 (1990)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Nozawa,K.Ohtomo,M.Suzuki,Y.Morishita,H.Konami and Michael T.Madigan: "Structures of Bacteriochlorophyll c's in Chlorosomes from a New Thermophilic Bacterium Chlorobium tepidum" Chem.Lett.1763-1766 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Nozawa,K.Ohtomo,N.Takeshita,Y.Morishita,M.Ohsawa and Michael T.Madigan: "Substituent Effects on the Aggregation of Bacteriochlorophyll d Homologues Purified from Chlorobium limicola" Bull.Chem.Soc.Jpn.65. 3493-3494 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Nozawa,K.Ohtomo,Y.Morishita,H.Konami and Michael T.Madigan: "Dimer Structures of Bacteriochlorophyll c from Chlorobium tepidum in CDCl_3" Chem.Lett.261-264 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Nozawa,K.Ohtomo,M.Suzuki,Y.Morishita and Michael T.Madigan: "Structures and Organization of Bacteriochlorophyll c's in Chlorosomes from a New Thermophilic Bacterium Chlorobium tepidum" Bull.Chem.Soc.Jpn.66. 1-7 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Nozawa,K.Ohtomo,M.Suzuki,Y.Morishita and H.Konami: "Progress in Photosynthesis Research CP/MAS ^<13>C NMR Studies on Antenna Structures in Chlorosomes in Green Bacteria" Kluwer Academic Publishers, 4 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tsunenori Nozawa,Manabu Suzuki,Syu Kanno,S.Shirai: "CP/MAS ^<13>CーNMR Studies on the Structure of Bacteriochloro phyll c in Chlorosomes from Chloroflexus aurantiacus" Chem.Lett.1805-1808 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Nozawa,M.Suzuki,K.Ohtomo,Y.Morishita,H.Konami,and Michael T.Madigan: "Aggregation Structure of Bacteriochlorophyll c in Chloro somes from Chlorobium tepidum" Chem.Lett.1641-1644 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Nozawa,K.Ohtomo,M.Suzuki,Y.Morishita,and Michael T.Madigan: "Structures of Bacteriochlorophyll c's in Chlorosomes from a New Thermophilic Bacterium Chlorobium tepidum" Chem.Lett.1763-1766 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Nozawa,K.Ohtomo,Y.Morishita,H.Konami and Michael T.Madigan: "Dimer Structures of Bacteriochlorophyll c from Chlorobium tepidum in CDCl_3" Chem.Lett.261-264 (1992)

    • Related Report
      1991 Annual Research Report

URL: 

Published: 1991-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi