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Three dimensional growth of animal cell by new immobilization technique and its application for artificial liver

Research Project

Project/Area Number 03650784
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 反応工学
Research InstitutionDepartment of Biotechnology, Nagoya University

Principal Investigator

IIJIMA Shinji  Professor Department of Biotechnology Nagoya University, 工学部, 教授 (00168056)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Takeshi  Professor Department of Biotechnology Nagoya University, 工学部, 教授 (10043324)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsArtificial organ / Liver cell / Immobilization / Endothelial cells / 肝美細胞 / 肝実質細胞 / 幼若肝実質細胞 / 肝細胞の培養
Research Abstract

In order to establish artificial liver, long term passage of liver cells has been tried. Using liver cells isolated by collegenase method, effects of several vitamins, and amino acids for cell maintenance were studied. Among them, proline which is main component of collagen, and activated ascorbate which is a cofactor of collagen biosynthesis, facilitated albumin biosynthesis at least for one week. On the other hand, super oxide dismutase which decompose toxic super oxide anion also facilitate albumin secretion.
We also studied the suitable materials for immobilization of liver cells and developed a macroporous fiber which bound to heparin or collagen as cell attachment ligands. Vero cell attached very well to both of matrix and cell density was more than 10^7/ml support. This result indicated that the support may be suitable for artificial liver construction.
For the construction of artificial blood vessel, we isolated human endothelial cells. By the infection of a temperature sensitive mutant of SV40 virus, life span of cells was elongated for more than 200days at 33゚C. These cells died at 37゚C since the temperature sensitive tumor protein was inactivated at this temperature. Since these cells expressed endothelial cell specific function, they are useful for the analyses of blood vessel related disorder.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] S.Iijima,M.Ishida,S.Nakajima-Iijima,T.Hishida,H.Watanabe,T.Kobayashi: "Immobilization of Human endothelial Cells by Origin-Defective Simian Virus 40 DNA." Agric.Biol.Chem.55,. 2847-2853 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 真野 隆,満田 伸二郎,熊沢 栄太郎,川西 悟生,飯島 信司,小林 猛: "アルギン酸とポリウレタンを用いた固定化動物細胞に対するゼラチン添加の効果" 化学工学論文集. 17. 931-936 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Kamihira,J.Wang,M.Hata,S.Iijima,T.Kobayashi: "Immobilization of Animal Cells Using Photo-Crosslinkable Resin." J.Ferment.Bioeng.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 寺嶋 修司,小川 達也,上平 正道,安田 公昭,飯島 信司,小林 猛: "多孔性セルロール担体によるハイブリドーマ細胞の固定化とモノクローナル抗体の連続生産," 生物工学会誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.Iijima, M.Ishida, S.Nakajima-Iijima, Y.Hishida, H.Watanabe and T.Kobayashi: "Immobilization of Human endoghelial Cells by Origin-Defective Simian Virus 40 DNA." Agric. Biol. Chem.55. 2847-2853 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Mano, S.Mitsuda, E.Kumazawa and G.kawanishi: "Effect of Gelatin Addition on Immobilized Alginate Gel Beads with Polyrethane for Mammalian Cells." Kagakukougaku Ronbunshu. 17. 931-936 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Kamihira, J.Wang, M.Hata, S.Iijima and T.Kobayashi: "Immobilization of Animal Cells Using Photo-Crosslinkable Resin." J. Ferment. Bioeng.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.Terashima, T.Ogawa, M.kamihira, K.Yasuda, S.Iijima and T.Kobayashi: "Immobilization of Hybridopma Cells with Macroporous Celluylosic Support and Improves Production of Monoclonal Antibldy." Hakkou Kougakukaishi.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 寺嶋 修司、小川 達也、上平 正道、安田 公昭、飯島 信司、小林 猛: "多孔性セルロース担体によるハイブリドーマ細胞の固定化とモノクローナル抗体の連続生産" 生物工学会誌.

    • Related Report
      1992 Annual Research Report
  • [Publications] Masamichi Kamihira,Jin Wang,Mikiko Hata,Shinji Iijima and Takeshi Kobayashi: "Immobilization of Animal Cells Using Photo-Crosslinkable Resin" Journal of Fermentation and Bioengineering.

    • Related Report
      1992 Annual Research Report
  • [Publications] 真野 隆: "アルギン酸とポリウレタンを用いた固定化動物細胞に対するゼラチン添加の効果" 化学工学論文集. 17. 931-936 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Iijima: "Immortalization of human endothelial cells by originーdefective simian virus 40 DNA" Agric.Biol.Chem.55. 2847-2853 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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