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BACTERIAL CHEMOSENSORY TRANSDUCTION AND DEVELOPMENT OF A RAPID ASSAY TECHNIQUE FOR CHEMOATTRACTANTS

Research Project

Project/Area Number 03650793
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 発酵工学
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

OHTAKE Hisao  HIROSHIMA UNIV., Fac. of Eng., Professor, 工学部, 教授 (10127483)

Co-Investigator(Kenkyū-buntansha) KATO Junichi  HIROSHIMA UNIV., Fac. of Eng., Research Associate, 工学部, 助手 (90231258)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsBacteria / Chemotaxis / Transducer / Attractant / Sensory / 微生物 / 刺激感知機能 / 行動活性物質
Research Abstract

We investigated the sensory transduction in Pseudomonas aeruginosa. A widely used method for measuring chemotactic responses in bacteria is the capillary assay technique. Although this assay technique provides quantitative information on chemotaxis, it is time-consuming and tends to show great variability from trial to trial. We developed, therefore, the computer-assisted version of the capillary assay technique so that the bacterial response to an attractant could be assessed within a few minutes.
With this quick technique, we found that P. aeruginosa strain PAOI exhibited phosphate taxis when grown under phosphate limitation. In parallel with alkaline phosphatase activity, the strength of phosphate taxis was observed to increase during growth under phosphate limitation. We isolated mutants constitutive for alkaline phosphatase. All of the mutants showed the response to phosphate, regardless of whether the cells were starved for phosphate. This result suggests that phosphate taxis in P … More .aeruginosa is under the control of the same set of regulatory genes as phoA. To study further this point, we isolated a phoB mutant of P. aeruginosa and examined its ability to respond to phosphate. However, the phosphate taxis of the phoB mutant was still inducible under phosphate limitation, showing the phosphate taxis is not under the regulatory control of phoB. We further attempted to clone P.aeruginosa genes that are involved in its sensory transduction. A hybridization probe was prepared using the S. typhimurium tar transducer gene. The probe hybridized well with the EcoRI-digested genomic DNA of P. aeruginosa strain PAOI, and 5.4-kb EcoRI DNA fragment, to which the probe strongly hybridized, was cloned into a high-copy-number plasmid pUC18. After subcloning, we determined the nucleotide sequence of a 1.98-kb region of the cloned fragment. Nucleotide sequence analysis showed that this fragment contained an open reading frame which shared a high degree of homology with that of S.typhimurium tar. Less

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Junichi KATO,Ayumi ITO Toshiyuki NIKATA Hisao OHTAKE: "Phosphate taxis in Pseudomonas aeruginosa" J.Bacteriol.174. 5149-5151 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Toshiyuki NIKATA Ken SUMIDA,Junichi KATO,Hisao OHTAKE: "Rapid method for analyzing bacterial behavioral responses to chemical stimuli" Appl.Environ.Microbiol.58. 2250-2254 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hisao OHTAKE Junichi KATO Toshiyuki NIKATA: "Sensory transduction in Pseudomonas aeruginosa" Proc.International Symposium on Advanced Computing for Life-Science. 167-169 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hisao OHTAKE HARDOYO: "New biological method for detoxification and removal of hexavalent chromium" Wat.Sci.Tech.25. 395-402 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 大竹 久夫: "細菌の刺激感知機能の迅速計測システム" バイオサイエンスとバイオインダスより. 50. 7-8 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hisao OHTAKE,Junichi KATO Toshiyuki NIKATA: "Biochemical Engineering for 2001(S.Furukaki,I.Endo and R.Matsuno(Eds.)" Springer-Verlag, 452 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Junichi KATO: "Phosphate taxis in Pseudomonas aeruginosa" J.Bacteriol. Vol.174. 5149-5151 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tosiyuki NAKATA: "Rapid method for analyzing bacterial behavioral responses to chemical stimuli" Appl.Environ.Microbiol. Vol.58. 2250-2254 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hisao OHTAKE: "Sensory transduction in Pseudomonas aeruginosa" Proc.International Symposium on Advanced Computing for Life-Science. 167-169 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hisao OHTAKE: "New biological method for detoxification and removal of hexavalent chromium" Wat.Sci.Tech. Vol.25. 395-402 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hisao OHTAKE: SPRINGER-Verlag. Biochemical Engineering for 2001(S. Furukaki, I. Endo and R.Matsuno(Eds.), 452 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Junichi KATO,Ayumi ITO Toshiyuki NIKATA Hisao OHTAKE: "Phosphate taxis in Pseudomonas aeruginosa" J. Bacteriol.174. 5149-5151 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Toshiyuki NIKATA Ken SUMIDA, Junichi KATO,Hisao OHTAKE: "Rapid method for analyzing bacterial behavioral responses to chemical stimuli" Appl. Environ. Microbiol.58. 2250-2254 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hisao OHTAKE Junichi KATO Toshiyuki NIKATA: "Sensory transduction in Pseudomonas aeruginosa" Proc.International Symposium on Advanced Computing for Life-Science. 167-169 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hisao OHTAKE HARDOYO: "New biological method for detoxification and removal of hexavalent chromium" Wat. Sci. Tech.25. 395-402 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 大竹 久夫: "細菌の刺激感知機能の迅速計測システム" バイオサイエンスとバイオインダストリ. 50. 7-8 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hisao OHTAKE,Junichi KATO Toshiyuki NIKATA: "Biochemical Engineering for 2001 (S.Furukaki,I.Endo and R.Matsuno(Eds.)" Springer-Verlag, 452 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Nikata,K.Sumida,J.Kato and H.Ohtake: "A rapid method for analyzing bacterial behavioral responses to chemical stimuli" Appl.Environ.Microbiol.

    • Related Report
      1991 Annual Research Report
  • [Publications] J.Kato,A.Ito,T.Nikata and H.Ohtake: "Phosphate taxis in Pseudomonas seruginosa" J.Bacteriol.

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Kitamura,T.Nikata,J.Kato and H.Ohtake: "Chemotaxis toward amino acids in Pseudomonas aeruginosa" J.Gen.Microbiol.

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Ohtake,J.Kato and T.Nikata: "A new method for analyzing bacterial activities to sense and respond to chemical stimuli" Proc.AsiaーPacific Biochemical Enginer.Symp.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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