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Genetic Study of Nitrate Transport across the Plasma Membrane of Root Epidermal Cells in Some Crop Plants

Research Project

Project/Area Number 03660007
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionUniversity of Osaka Profecture

Principal Investigator

HASEGAWA Hiroshi  University of Osaka Prefecture, Assistant Professor, 附属研究所, 講師 (00090457)

Co-Investigator(Kenkyū-buntansha) INOUE Masayoshi  Kyoto Prefectural University, Professor, 農学部, 教授 (90090456)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
KeywordsNitrate uptake / Barley / Rice / Wheat / Km and Vmax / Mutant / Low-input cultivation / Seedling / 誘導時間 / 温度 / ライムギ
Research Abstract

Breeding of crops with efficient use of plant nutrient is one of the goals for low-input crop production. In this study, two kinetic parameters, Km and Vmax, for high affinity nitrate uptake by young seedlings were determined in several crop plants for evaluating the expression of nitrate uptake gene (s). Summary of the results are shown as follows.
1) In twenty Japanese barley cultivars, Km and Vmax were 50.9-111.7 mu M and 2.24-4.99 mu mol/g/h, respectively. IIowever, these results were thought to be underestimated because of experimental procedure. 2) In barley genotypes with different water tolerance at seedling stage, Km and Vmax were 143.0-209.4 mu M and 3.37-7.85 mu mol/g/h, respectively. No relationships between the kinetic parameters and water tolerance was observed. 3) In sixteen rice cultivars including both indica and japonica, Km and Vmax were 75.7-130.3 mu M and 4.30-7.17 mu mol/g/h, respectively. In the cultivars of indica and japonica showing similar level of Km, indica cultivars tended to show lower Vmax than japonica cultivars. 4) Km and Vmax in several genotypes of wheat, rye and Triticale were similar level of those of barley and rice. 5) In four rice mutants affecting nitrate assimilation, M605, a chlorate hypersensitive mutant, showed significantly higher Km than that of the wild type, Norin 8. Furthermore, there was difference in ratio of nitrate uptake ability from 40 mu M KNO_3 to that from 400 mu M KNO_3 in 145 rice genotypes, suggesting variability in the kinetic parameters.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Masahiko ICHII,Toyomasa KATAGIRI and Hiroshi HAEGAWA: "Mutants with low nitrate reductase activity selected from seedlings expressing nitrogen deficiency symptoms in rice COryza sativa L-3" Japanese Journal of Breeding. 43. 123-127 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 長谷川博・矢頭治・一井真比古: "塩素酸抵抗性・感受性を指標とした作物の硝酸代謝能の評価" 近畿作物の育種研究. 38. 21-25 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 森田和恵・長谷川博・中村明夫: "タバコ葯培養に及ぼすガンマ線の効果" 近畿作物育種研究. 38. 61-64 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 中村明夫・高島 賢・長谷川 博・松本 豪: "タバコ半数体幼苗のコルヒチン処理による簡易で効率的な染色体倍加法" 育種学雑誌. 43. 603-612 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masahiko ICHll,Toyomasa KATAGIRI and Hiroshi HAEGAWA: "Mutants with low nitrate reductase activity selected from seedlings expressing nitrogen deficiency symptoms in rice coryza sativa〓‐)" Japanese Journal of Breeding. 43. 123-127 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 長谷川博・矢頭治・一井真比古: "塩素酸抵抗性・感受性を指標とした作物の硝酸代謝能の評価" 近〓作物育種研究. 38. 21-25 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 森田和恵・長谷川博・中村明夫: "タバコ葯培養に及ぼすガンマ線の効果" 近〓作物育種研究. 38. 61-64 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 中村明夫・高島賢・長谷川博・松本豪: "タバコ半数体幼苗のコルヒチン処理による簡易で効率的な染色体倍加法" 育種学雑誌. 43. 603-612 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Hasegawa,T.Katagiri,S.Ida,O.Yatou and M.Ichi: "Characterization of a rice(Oryza sativa L.)mutant deficient in the heme domain of nitrate reductase" Theoretical and Applied Genetics. 84. 6-9 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 長谷川 博: "わが国オオムギ品種における硝酸イオン吸収のミハエリス=メンテン式による解析" 日本作物学会紀事. 61. 251-256 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 長谷川 博・川崎 俊己 市橋 秀樹・渡辺 洋: "数種作物の根における硝酸イオンキャリアーの特性.ミハエリス=メンテン定数および誘導時間について." 近畿作物・育種研究. 37. 95-101 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 長谷川 博: "わが国オオムギ品種における硝酸イオン吸収のミハエリス=メンテン式による解析" 日本作物学会紀事. 61. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 長谷川 博,川崎 俊己,市橋 秀樹,渡辺 洋: "数種作物の根における硝酸吸収キャリア-の特性。ミハエリス=メンテン定数および誘導時間について。" 近畿作物・育種研究. 37. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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