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Studies on spore dimorphism of the microsporidia

Research Project

Project/Area Number 03660057
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 蚕糸学
Research InstitutionNihon University

Principal Investigator

ISHIHARA Ren  Nihon Univ. Agri. & Vet. Med. Prof., 農獣医学部, 教授 (10058852)

Co-Investigator(Kenkyū-buntansha) IWANO Hidetoshi  Nihon Univ. Agri. & Vet. Med. Lecturer, 農獣医学部, 講師 (40120381)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsNosema bombycis / Nosema sp. / Nosema furnacalis / Bombyx mori / Spodoptera deprauata / Microsporidia / spore dimorphism / Vairimorpha sp. / spore dimorphism / 微粒子病
Research Abstract

The purpose of present research was to make sure the development of two types of spores of Nosema bombycis in cells of the silkworm larvae, and examine other microsporidia whether they produce the two types of spores or not. Those spores were discovered first in cultured cells after N . bombycis was inoculated. One type of those spors is characterized by 3 to 5 coils of the polar tube, early development (36 h postinoculation), and the spontaneous discharge of a short polar tube. The other is characterized by 10 to 12 coils of the polar tube and the late development (54 h postinoculation).
The results are summerized as below, 1) The two types of spores were confirmed to develop in midgut epithelial cells of the silkworm larvae after peroral inoculation. 2) A new Nosema sp. was isolated from the lawn grass cutworm, Spodoptera deprauata. This microsporidium produced two types of spores exactly the same ones as found in N.bombycis, while it did not infect the silkworm by oral administration of spores, and its spores did not react with latex particles whic had been sensitized with a monoclonal antibody against spores of N.bombycis. This microsporidium had two nuclei in diplokaryotic arrangement throughout its development. A meront gave rise to two spores, and there was no sporophorous vesicle. Hence it is another Nosema sp. isolated from S.deprauata. 3) We also identified the two types of spores in N.furnacalis developing in cultured cells. Furthermore, one of spore, early developing one, was isolated and successfully inoculated onto cultured cells, giving high rate of infection.
These results indicate that this spore dimorphism is a character shared by microsporidia belonging to the genus Nosema.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 岩野秀俊,石原廉: "家蚕幼虫体内におけるノゼマ・ボンビシス胞子二型の形成" 日本蚕糸学雑誌. 60. 249-256 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 岩野秀俊,石原廉: "Nosema bombycisのスジキリヨトウ成虫からの分離." 日本蚕糸学雑誌. 60. 279-287 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 石原廉,岩野秀俊: "家蚕微粒子病病原の保有宿主としてのスジキリヨトウ." 日本蚕糸学雑誌. 60. 236-237 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 早坂昭二,石原廉: "カイコの微胞子虫Vairimorpha sp.M-12の病原性に及ぼす継代宿主の影響." 蚕糸研究. 5. 17-20 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hidetoshi IWANO,Nobuaki SHIMIZU,Yuji KAWAKAMI and Ren ISHIHARA: "Spore dimorphism and some other biological features of a Nosema sp.isolated from the lawn grass cutworm.Spodoptera depravata Butler" Applied Entomology and Zoology. 29(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kawakami,U.,Inoue,T.,Shimizu,T.,Takayanagi,M.,Sunairi,M.,Ando,T.,and Ishihara,R.: "Primary and secondary structures of 5S ribosomal RNA of Nosema bombycis(Nosematidae,Microsporidia)" J.Sericult.Sci.Jpn.61. 321-327 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Iwano, H.and Ishihara, R.: "Isolation of Nosema bombycis from moths of the lawn grass cutworm, Spodoptera depravata Butler. (in Japanese)" J.Sericult.Sci.Jpn. 60. 279-287 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Iwano, H.and Ishihara, R.: "Dimorphic development of Nosema bombycis spores in gut epithelium of larvae of the silkworm, Bombyx mori. (in Japanese)" J.Sericult.Sci.Jpn. 60. 249-256 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ishihara, R.AND Iwano, H.: "The lawn grass cutworm, Spodoptera depravata Butler, as a natural reservoir of Nosema bombycis Naegeli. (in Japanese)" J.Sericult.Sci.Jpn. 60. 236-237 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Iwano, H., Shimizu N., Kawakami, U.and Ishihara, R.: "Spore dimorphism and some other biological features of a Nosema sp. isolated from the lawn grass cutworm, Spodoptera depravata Butler in press" Appl. Entomol. Zool. 29. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hayasaka, S.and Ishihara, R.: "Influence of host insect on the pathogenicity of microsporidilan Vairimrpha sp. M12 derived from silkwrom, Bombyx mori (in Japanese)" Acta Seric. Entomol. 5. 17-20 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kawakami, U., Inoue, T., Ito, K., Kitamizu, K., Hanawa, C., Sunairi, M., Ando, T., Iwaano, H., and Ishihara, R.: "Comparison of chromosomal DNA from four microsporidia pathogenic to the silkworm (in press)" Appl. Entomol. Zool. 29. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kawakami, U., Inoue, T., Schimizu, T., Takayanagi M., M., Sunairi, M., Ando, T., and Ishihara, R.: "Primary and secondary structures of 5S ribosomal RNA of Nosema bombycis (Nosematidae, microsporida)" J.Sercult.Sci.Jpn. 61. 321-327 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 岩野秀俊、石原廉: "家蚕幼虫体内におけるノゼマ・ボンビシス胞子二型の形成" 日本蚕糸学雑誌. 60. 249-256 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 岩野秀俊・石原廉: "Nosema bombycisのスジキリヨトウ成虫からの分離." 日本蚕糸学雑誌. 60. 279-287 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 石原廉・岩野秀俊: "家蚕微粒子病病原の保有宿主としてのスジキリヨトウ." 日本蚕糸学雑誌. 60. 236-237 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 早坂昭二・石原廉: "カイコの微胞子虫Vairimorpha sp.M-12の病原性に及ぼす継代宿主の影響." 蚕糸研究. 5. 17-20 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hidetoshi IWANO.Nobuaki SHIMIZU.Yuji KAWAKAMI and Ren ISHIHARA: "Spore dimorphism and some other biological features of a Nosema sp.isolated from the lawn grass cutworm.Spodoptera depravata Butler" Applied Entomology and Zoology (in press). 29. ??? (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kawakami,U.,Inoue,T.,Shimizu,T.,Takayanagi,M.,Sunairi,M.,Ando,T.,and Ishihara,R.: "Primary and secondary structures of 5S ribosomal RNA of Nosema bombycis(Nosematidae,Microsporidia)" J.Sericult.Sci.Jpn.61. 321-327 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 岩野 秀俊: "家蚕幼虫体内におけるNosema bonbycis胞子二型の形成" 日本蚕糸学雑誌. 60. 249-256 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岩野 秀俊: "Nosema bombycis以外のNosema属微胞子虫における胞子二型の形成" 日本蚕糸学雑誌.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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