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Cellular Biological Analysis of Secretion Mechanism under Nutrient Stress.

Research Project

Project/Area Number 03660065
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 土壌・肥料
Research InstitutionThe University of Tokyo

Principal Investigator

NISHIZAWA Naoko  The Univ. of Tokyo Agriculture assistant, 農学部, 助手 (70156066)

Co-Investigator(Kenkyū-buntansha) MORI Satoshi  The Univ. of Tokyo, Agriculture associate professor, 農学部, 助教授 (90011915)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsMugineic Acid / Nicotianamine / Iron-deficiency stress / Barley / オオムギ根
Research Abstract

Fe-deficiency induces the biosynthesis of mugineic acid family phytosiderophores (MAs) in graminaceous plants. Using a cell free system derived from root tips of iron deficient barley, we clarified the biosynthetic pathway of deoxymugineic acid (DMA) from methionine in vitro [ Methionine S-adenosyl methionine Nicotianamine(NA) (3"-ketoform) DMA].
In order to isolate the genes encoding the biosynthetic enzymes of MAs, we tried to purify the enzymes which synthesize nicotianamine from SAM (NA synthase) and unidentified 3"-ketoform from NA. They were partially purified by using hydrophobic chromatography, gel filtration, ion exchange, and native electrophoresis.
The characteristics of both enzymes clarified until now are as fallows.
1. Biosynthetic activity of both processes were markedly induced by iron deficiency stress.
2. Both enzymes show the same optimum PH (9).
3. Molecular weight of NA synthase is 40-50KD.
4. The enzyme synthesizing 3"-ketoform needs 2-oxoglutarate and pyridoxal phosphate (PLP) as a cofactor, and its molecular weight is about 80KD.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] NISIZAWA,N.K.: "Zxpression of an Iron-deficiency Specific Clone(Ids 3)in Roots of Hordeum Vulgare" Plant & Cell Physiol.34. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] NISHIZAWA,N.K.: "Iron Deficiency Specific cDNA (Ids 1)with two Homo-logous Cysteinle rich MT Domains from the Roots of Barloy" J.of Plant Nutrition. 15. 2157-2172 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] MORI,S.: "Development and Characterization of a Monoclonal Antibody to Phytosiderophores." Plant & Call Physiol. 33. 151-156 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] MORI,S.: "Iron Extraction Efficiency of Plant borne Mugineic Acid Family Phytosideropbores in Indian Calcorious Soils" J.of Plant Natrition. 15. 1625-1645 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] NISHIZAWA,N.K.: "An Iron-Deficiency Specific cDna from barely roots baving two homologous Cystein-ricli MT Domains." Plant Molecular Biology. 17. 531-533 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] NISHIZAWA,N.K.: "Cross-reactivities of Arcti-nucotiananine antibody with mugineic aud and Related Conpounds" Soil Sci.Plant Natri.37. 159-162 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Okumura, N., Nishizawa, N. K., Umehara, Y., Ohata, T., Mori, S.: "Iron deficiency specific cDNA (Ids 1) with two homologous cysteine rich MT domains from the roots of barley." J. of Plant Nutrition. 15. 2157-2172 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Mihashi, S., Sikora, L. J., Buyer, J. S., Fushiya, S., Mori, S.: "Development and characterization of a monoclonal antibody to phytosiderophores." Plant & Cell Physiol.33. 151-156 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Singh, K., Chino, M., Nishizawa, N. K., Goto, T., Nakanishi, T., Takagi, S., Mori, S.: "Iron extraction efficiency of plant borne mugineic acid family phytosiderophores in indian calcarious soils." J. of Plant Nutrition. 15. 1625-1645 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Okumura, N., Nishizawa, N. K., Umehara, Y., Mori, S.: "An iron deficiency-specific cDNA from barley roots having two homologous cysteine-rich MT domains." Plant Molecular Biology. 17. 531-533 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shojima, S., Nishizawa, N. K., Fushiya, S., Nozoe, S., Takagi, S., Mori, S., Chino, M.: "Cross-reactivities of Anti-nicotianamine antibody with mugineic acid and Related compounds." Soil Sci. Plant Nutr.37. 159-162 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakanishi, H., Okumura, N., Umehara, Y., Nishizawa N. K., Chino, M, Mori, S.: "Expression of an Iron-deficiency specific clone (Ids 3) in roots of Hordeum vulgare." Plant & Cell Physiol.34.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nishizawa,N.K.: "Expression of an Iron-deficiency Specific clone(Ids3) in Roots of Hordeum vulgare" Plent & Cell Physiol.34. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nishizawa,N.K.: "Iron Deficiency Specific cDNA(Ids1) with two Homo-logous Cysteine rich MT Domains from the Roots of Barley" J.of Plant Nutrition. 15. 2157-2172 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Mori,S.: "Develapment and Cbaracterization of a Monoclonal Anitibody to Phytosideropbores." Plant & Cell Physiol. 33. 151-156 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Mori,S.: "Iron Extraction Efficiency of Plant borne Mugineic Acid Family Phytosideropbores in Indian Calcarious Soils" J.of Plant Nutrition. 15. 1625-1645 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nishizawa,N.K.: "An Iron-deficiency Specific cDNA from barely roots Baving two homo lo gous Cyutein-rich MT Domains." Plant Molecular Biology. 17. 531-533 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nishizawa,N.K.: "Cross-reactivities of Anti-micotianamine antibody with mugineic acid and Related Compounds" Soil Sci.Plant Nutri.37. 159-162 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nishizawa,N.K.: "Crossーreactivities of Antiーnicotianamine antibody with mugineic acidaud Related compounds." Soil Sci.Plaut Nutri.37. 159-162 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nishizawa,N.K.: "An iron deficiencyーspecific cDNA from barley roots having two homologous cysteineーrich MT domains." Plant Molecular Biology. 17. 531-533 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nishizawa,N.K.: "ImmunoーcytoChemical detection of mugineic acid in Feーdeficient barley root cells." Abst.VIth Int.Iron Symp.(1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nishizawa,N.K.: "Why are young rice plants highly susceptible to Feーdeficiency." Plant&Soil. 130. 143-156 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mori,S.: "Enhancement of ferricーmugineic acid up take by iron deficient barley roots in the preseuce of excess free nugineic acid in the medium." Plant&Soil. 130. 135-141 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mori,S.: "Development and Characterization of a Monoclonal Antibody to phytosiderophores." Plaut&Cell Physiol.(1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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