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Functions and behavior of a new type of ATPase(phosphatase) in halophytes

Research Project

Project/Area Number 03660087
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionKinki University

Principal Investigator

HIRAYAMA Osamu  Kinki University, Food Nutrition, Professor, 農学部, 教授 (80032580)

Co-Investigator(Kenkyū-buntansha) 横田 一成  島根大学, 農学部, 助教授 (90158361)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordshalophyte / ATPase / phosphatase / membrane-bound enzyme / salt-resistant enzyme / ミクロソ-ム / 膜結合型酵素
Research Abstract

A new type of ATPase or phosphatase was found in a halophyte, Solsola komaroli. It occurred in two forms of plasma membrane-bound and free, their activity ratio being 4/96. Both forms of the enzyme hydrolyzed phosphate ester of substrates in the following order ; p-nitrophenylphosphate > pyrophosphate > ADP * ATP > AMP. The activities of both enzymes were stimulated by mono- and di-valent cations, and they were strongly inhibited by molybdate and vanadate. From these properties, this enzyme should be accepted as a new type of acid phosphatase. Both forms of the enzyme were resistant to salinity, particularly the membrane-bound form was stimulated by high concentrations of salt. The present enzyme was also found in non-halophytes, soybean and cucumber, and it was distributed at an increasing concentration with an increase in salt tolerance of plants.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] O.HIRAYAMA,M.NODA and T.TETSUO: "Characterization and distributation of acid phosphatase in a halophyte,saltwort" Phytochemistry,.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] O.HIRAYAMA and K.NAGAOKA: "Separation of inside-out and right-side-out membrane vesicles from plasma- and tonoplast-membranes of soybean and saltwort" Plant cell Physiol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] O.Hirayama,M.Noda and T.Tetsuo: "Characterization and distribution of acid phosphatase in a halophyte,saltwort" Phytochemistry,.

    • Related Report
      1992 Annual Research Report
  • [Publications] O.Hirayama and K.Nagaoka: "Separation of inside-out and right-side-out membrane vesicles from plasma-and tonoplast-membranes of soybean and saltwort" Plant cell Physiol.

    • Related Report
      1992 Annual Research Report
  • [Publications] 平山 修,横田 一成,野田 政之,本多 哲郎: "塩生植物オカヒジキにおける膜結合性ホスファタ-ゼの存在とその特性" Agricultural and Biological chemistry.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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