Functions and behavior of a new type of ATPase(phosphatase) in halophytes
| Project/Area Number |
03660087
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用生物化学・栄養化学
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| Research Institution | Kinki University |
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Principal Investigator |
HIRAYAMA Osamu Kinki University, Food Nutrition, Professor, 農学部, 教授 (80032580)
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| Co-Investigator(Kenkyū-buntansha) |
横田 一成 島根大学, 農学部, 助教授 (90158361)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | halophyte / ATPase / phosphatase / membrane-bound enzyme / salt-resistant enzyme / ミクロソ-ム / 膜結合型酵素 |
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| Research Abstract |
A new type of ATPase or phosphatase was found in a halophyte, Solsola komaroli. It occurred in two forms of plasma membrane-bound and free, their activity ratio being 4/96. Both forms of the enzyme hydrolyzed phosphate ester of substrates in the following order ; p-nitrophenylphosphate > pyrophosphate > ADP * ATP > AMP. The activities of both enzymes were stimulated by mono- and di-valent cations, and they were strongly inhibited by molybdate and vanadate. From these properties, this enzyme should be accepted as a new type of acid phosphatase. Both forms of the enzyme were resistant to salinity, particularly the membrane-bound form was stimulated by high concentrations of salt. The present enzyme was also found in non-halophytes, soybean and cucumber, and it was distributed at an increasing concentration with an increase in salt tolerance of plants.
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Report
(3 results)
Research Products
(5 results)
