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Genetic and enzymatic analyses of C-P bond cleavage enzyme in bacteria

Research Project

Project/Area Number 03660113
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・発酵学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MURATA Kousaku  Kyoto University Res. Inst. Food Sci. Associate professor, 食糧科学研究所, 助教授 (90142299)

Co-Investigator(Kenkyū-buntansha) INOUE Yoshiharu  Kyoto University Res. Inst. Food Sci. Research Associate, 食糧科学研究所, 助手 (70203263)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1992: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1991: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsC-P bond / C-P bond cleavage enzyme / C-P hydrolase / C-P lyase / CーP結合 / CーP結合解裂酵素 / CーP結合加水分解酵素 / CーPリア-ゼ
Research Abstract

A direct carbon-phosphorus (C-P) bond is highly stable and resistant to chemical hydrolysis, thermal decomposition and photolysis. Therefore, biological cleavage of the C-P bond in natural habitats assumes importance as a mean to prevent the accumulation of phosphonates. Some bacterial cells are known to use various phosphonates for growth. However, all attempts to detect C-P bond cleavage activity in cell-free systems have resulted in failure, and the problem of how and by what means these bacteria can cleave the C-P bond in phosphonates remains unsolved. We have succeeded, for the first time, in the detection of C-P bond cleavage activity in cell-free extracts of a bacterium Enterobacter aerogenes, and isolated an enzyme responsible for the activity. The enzyme catalyzed the C-P bond cleavage and produced inorganic phosphate. Formation of alkane was not observed. The enzyme was then designated C-P hydrolase to descriminate from C-P lyase and phosphonatase. The enzyme required two proteins (E2 and E3) for activity. E2 was 650 kDa in molecular size. The function of E2 is to convert stable C-P bond into unstable one. E3 was a homodimer of a subunit with M.W. of 55 kDa. The function of the protein is to hydrolyze C-P bond being unstabled by E2 protein. The entire gene covering E2 and E3 structural regions was cloned from the organism and its structure was also analyzed.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Kousaku Murata: "Detection of carbon-phosphorus lyase activity in cell extracts of Enterobacter aerogenes" Biochemical and Biophysical Research Communication. 157. 190-195 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kousaku Murata: "Carbon-phoaphorus hydrolase:Some properties of the enzyme in cell extracts of Enterobacter aerogenes" Ageicultural and Biological Chemistry. 53. 1225-1229 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kousaku Murata: "A microbial carbon-phosphorus bond cleavage enzyme requires two protein components for activity" Journal of Bacteriology. 171. 4504-4506 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kousaku Murata: "Carbon-phosphorus hydrolase:Functional association of two different components for the enzyme activity in Enterobacter aerogenes" Agricultural and Biological Chemistry. 53. 1419-1420 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nobue Osamura: "Cytosolic C-P bond cleavage activity in bacterial cells isolated from soil" Journal of Fermentation and Bioengineering. 71. 128-130 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 村田 幸作: "細菌のC-P結合解裂酵素" 日本生化学会誌. 64. 100-105 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kousaku Murata, Noriko Higaki and Akira Kimura: "Detection of carbon-phosphorus lyase activity in cell free extracts of Enterobacter aerogenes." Biochem. Biophys. Res. Commun.157. 190-195 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kousaku Murata, Noriko Higaki and Akira Kimura: "Carbon-phosphorus hydrolase: Some properties of the enzyme in cell extracts of enterobacter aerogenes" Agric. Biol. Chem.,. 53. 1225-1229 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kousaku Murata, Noriko Higaki and Akira Kimura: "A microbial carbon-phosphorus bond cleavage enzyme requires two protein components for activity." J. Bacteriol.171. 4504-4506 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kousaku Murata, Noriko Higaki and Akira Kimura: "Carbon-phosphorus hydrolase: Functional association of two different components for the enzyme activity in Enterobacter aerogenes." Agric. Biol. Chem.,. 53. 1419-1420 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nobue Osamura, Kousaku Murata and Akira Kimura: "Cytosolic C-P bond cleavage activity in bacterial cells isolated from soil." J. Ferment. Bioeng.71 (2). 128-130 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kousaku Murata: "C-P bond cleavage enzyme in bacteria." Biochemistry (Tokyo). 64(2). 100-105 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kousaku Murata: "Detection of carboh-phosphorus lyase activity in cell extracts of Enterobacter aerogenes" Biochemical and Biophysical Research Communication. 157. 190-195 (1988)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kousaku Murata: "Carbon-Phosphorus hydrolase:Some properties of the enzyme in cell extracts of Enterobacter aerogenes" Agricultural and Biological Chemistry. 53. 1225-1229 (1989)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kousaku Murata: "A microbial carbon-phosphorus bond cleavage enzyme requires two protein components for activity" Journal of Bacteriology. 171. 4504-4506 (1989)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kousaku Murata: "Carbon-phosphorus hydrolase:Functional asso-ciation of two different components for the enzyme activity in Enterobacter aerogenes" Agricultural and Biological Chemistry. 53. 1419-1420 (1989)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nobue Osamura: "Cytosolic C-P bond cleavage activity in bac-terial cells isolated from soil" Journal of Fermentation and Bioengineering. 71. 128-130 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 村田 幸作: "細菌のC-P結合解裂酵素" 日本生化学会誌. 64. 100-105 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kousaku Murata: "Detection of carbonーphosphorus lyase activity in cell free extracts of Enterobacter aerogenes" Biochemical and Biophysical Research Communication. 157. 190-195 (1988)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kousaku Murata: "Carbonーphosphorus hydrolase:Some properties of the enzyme in cell extracts of Enterobacter aerogenes" Agricultural and Biological Chemistry. 53. 1225-1229 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kousaku Murata: "Carbonーphosphorus hydrolase:Functional association of two different components for the enzyme activity in Enterobacter aerogenes" Agricultural and Biological Chemistry. 53. 1419-1420 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kousaku Murata: "A microbial carbonーphosphorus bond cleavage enzyme requires two protein components for activity" Journal of Bacteriology. 171. 4504-4506 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] 福田 民子: "CーP結合を切断する酵素" 日本醗酵工学会誌. 68. 44 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kousaku Murata: "CーP bond cleavage enzyme in bacteria" Bulletin of Research Institute for Food Science,Kyoto University. 53. 53-55 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 村田 幸作: "細菌のCーP結合解裂酵素" 日本農芸化学会誌. 65. 1501-1504 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nobue Osamura: "Cytosolic CーP bond cleavage activity in bacterial cells isolated from soil" Journal of Fermentation and bioengineering. 71. 128-130 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 村田 幸作: "細菌のCーP結合解裂酵素" 日本生化学会誌. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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