| Project/Area Number |
03660121
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用微生物学・発酵学
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| Research Institution | NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES |
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Principal Investigator |
YAGI Osami NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES WATER AND SOIL ENVIRONMENT DIVISION SECTION CHIEF, 水土壌圏環境部, 室長 (40132865)
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| Co-Investigator(Kenkyū-buntansha) |
IWASAKI Kazuhiro ibid.RESEARCHER, 地域環境研究グループ, 研究員 (30193717)
TOMIOKA Noriko ibid.RESEARCHER, 水土壌圏環境部, 研究員 (40168399)
UCHIYAMA Hiroo ibid.CHIEF RESEARCHER, 水土壌圏環境部, 主任研究員 (00185042)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | VOLATILE ORGANOCHLORINATED COMPOUNDS / SOIL CLEANUP / GROUNDWATER POLLUTION / TRICHLOROETHYLENE / TETRACHLOROETHYLENE |
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| Research Abstract |
A methane-utilizing bacterium, Methylocystis sp. M (strain M) was isolated from soil. This strain degrades trichloroethylene(TCE). Strain M can degrade TCE at a relatively high concentration fo 35 mg/L and as well as various halogenated aliphatic compounds. Bioremediation of TCE-contaminated soil by strain M was investigated in soil microcosms, using 70 ml of serum bottles and 450-ml glass columns as the soil microcosms. Strain M was added to the microcosms filled with a TCE-contaminated soil and water mixture with TCE concentration ranging from 0.1 to 1 ppm. More than 95% of TCE of 0.1 ppm was degraded by the addition of strain M, and degradation of 1,1-, cis- and trans-dichloroethylene (DCE) was accelerated by the strain. H_2O_2 addition with strain M significantly promoted TCE degradation. Strain M proved to be very effective to cleaning up the TCE-contaminated soil.
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