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Design and Improvement of Food Proteins by the Introduction of New Function

Research Project

Project/Area Number 03660134
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 製造化学・食品
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

YOSHIKAWA Masaaki  Kyoto Univ., Dept. Food Sci. & Technol., Assoc. Prof., 農学部, 助教授 (50026572)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsfood protein / casein / peptide / casoxin / bradykinin / endothelium / opioid / gluten
Research Abstract

Biologically active peptides are screened for in enzymatic digests of food proteins. Derivatives of theses peptides were synthesized and their structure-activity relationships were studied to design orally effective biologically active peptides, which could be introduced into food proteins by protein engineering. Casoxin C, Tyr- Ile-Pro-Ile-Gln-Tyr-Val-Leu-Ser-Arg, which is released from bovine kappa-casein by the action of trypsin is a multifunctional peptide. Homology is found between the penta- peptide sequence at the carboxyl terminus of casoxin C and the laminin penta-peptide, Tyr-Ile-Gly-Ser-Arg. Unlike laminin penta-peptide, casoxin C or pepta-peptide at its carboxyl terminus did not inhibit tumor metastasis. The [Ile^7,Gly^8]-casoxin C, which has laminin penta-peptide sequence at the carboxyl terminus inhibited tumor metastasis in mice.
Casoxin D, Tyr-Val-Pro-Phe-Pro-Pro-Phe, is a vaso-relaxing peptide derived from human alpha_<51>-casein. Casoxin D binds to bradykinin BK1-receptors of endothelium. The endothelium-derived relaxing factor released by casoxin D stimulusis is prostacyclin. Among casoxin D derivatives synthesized, Tyr-Pro-Phe-Pro-Pro-Phe, Val-Val-Phe-Pro- Pro-Phe and Tyr-Val-Phe-Pro-Pro-Phe showed vaso-relaxing activity at the concentrations 1/4, 1/7 and 1/10 that of casoxin D. Tyr-Pro-Phe-Pro-Pro-Phe lowered blood pressure of spontaneously hypertensive rats after oral administration while other peptides were active only after intravenous administration. Another derivative Tyr- Pro-Phe-Pro-Pro-Leu showed vaso-relaxing activity at the concentration 1/10,000 that of casoxin D. Mode of action of this peptide was different from that of casoxin D.
Opioid peptides were isolated from enzymatic digests of wheat gluten and potent derivatives were obtained by substitution of single amino acid residues.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] S.FUKUDOME: "Opioid Peptides Derived form Wheat Gluten:Their Isolation and Character ization." FEBS Lett. 296. 107-111 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.YOKOYAMA: "Peptid Inbibitors for Angiotensin I-converting Enzyme from Thermolysin Digest of Dried Bonito." Biosci.Biotech.Biochem.56. 1541-1545 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 吉川 正明: "ランダムペプチドライブラリー-活性ペプチドの新しいスクリーニング系" 化学. 47. 72-73 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.FUKUDOME: "Gluten Exorphin C,A Nobel Opioid Peptide Derived from Wheat Gluten." FEBS Lett.316. 17-19 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.YOSIKAWA: "Encycloped ia of Food Science and Technology" Wiley & Song Inc., 6 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.YOSIKAWA: "Frontiers and New Horizons in AminoAc id Research." Elsevier Science Publishers ., 7 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S. Fukudome and M. Yoshikawa,: "Opioid Peptides Derived from Wheat Gluten: Their Isolation and Characterization" FEBS Lett.296. 107-111 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K. Yokoyama, H. Chiba and M. Yoshikawa,: "Peptide Inhibitors for Angiotensin I-converting Enzyme from Thermolysin Digest of Dried Bonito" Biosci., Biotech., Biochem.56(10). 1541-1545 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M. Yoshikawa: "Random Peptide Libraries, a New Screening System for Bio-active Peptides (in Japanese)" Kagaku. 7(12). 72-73 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S. Fukudome and M. Yoshikawa,: "Gluten Exorphin C, A Nobel Opioid Peptide Derived from Wheat Gluten" FEBS Lett.316(1). 17-19 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M. Yoshikawa and H. Chiba: Willey & Sons Inc.Peptides "Encyclopedia of Food Science and Technology", 2043-2048 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M. Yoshikawa and H. Chiba: Elsevier Science Publishers. Biologically Active Peptides Derived from Food and Blood Proteins "Frontiers and New Horizons in Amino Acid Research", 403-409 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.Fukudome: "Opioid Peptides Derived from Wheat Gluten:Their Isolation and Characterization." FEBS Lett.296. 107-111 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Yokoyama: "Peptide Inhibitors for Angiotensin I-converting Enzyme from Thermolysin Digest of Dried Bonito." Biosci.Biotech.Biochem.56. 1541-1545 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 吉川 正明: "ランダムペプチドライブラリー-活性ペプチドの新しいスクリーニング系" 化学. 47. 72-73 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Fukudome: "Gluten Exorphin C, A Nobel Opioid Peptide Derived from Wheat Gluten." FEBS Lett.316. 17-19 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Yoshikawa: "Encyclopedia of Food Science and Technology" Wiley & Sons Inc., 6 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Yoshikawa: "Frontiers and New Horizons in Amino Acid Research." Elsevier science Publishers., 7 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Fukudome: "Opioid Peptides Derived from Wheat Gluten:Their Isolation and Characterization" FEBS Lett.296. 107-111 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Yokoyama: "Peptide Inhibitors for Angiotensin IーConverting Enzyme from Thermolysin Digest of Dried Bonito" Biosci.Biotech.Biochem.56. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Yoshikawa: "Amino Acid Research,Frontiers and New Horizons" Elsevier, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Yoshikawa: "βーCasomorphin and Related Peptides" Spektrum Akademischer Verlag, (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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