| Project/Area Number |
03660190
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General fisheries
|
|---|
| Research Institution | Mie University |
|---|
Principal Investigator |
HIDAKA Iwao Mie University, Faculty of Bioresources, Professor, 生物資源学部, 教授 (80046341)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MORISHITA Tatsuo Mie University, Faculty of Bioresources, Professor, 生物資源学部, 教授 (30024839)
|
|---|
| Project Period (FY) |
1991 – 1992
|
| Project Status |
Completed (Fiscal Year 1992)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Keywords | Fish / Yellowtail / Feeding stimulants / Extracts / Taste |
|---|
| Research Abstract |
The feeding-stimulatory effectiveness of a water extract of the jack mackerel Trachurus japonicus white muscle for the young yellowtail Seriola quinqueradiata was studied with the aid of gel chromatography, ion exchange chromatography and other analytical methods. The feeding-stimulatory effectiveness of test fractions of the extract was evaluated by applying them to the young yellowtail in starch pellets and counting the number of pellets eaten by the fish. (1) Gel fractions of the extracts on a column of Sephadex-15 The water extract of the muscle was ultrafiltrated through a filter membrane (UK-15, Advantic, M.W. cut off 10,000) and the ultrafiltrate was fractionated on a column of Sephadex G-15 from 0.05 M sodium chloride. The fractions obtained were divided into groups and pooled. Then the pooled eluate was freeze-dried and the residues were dissolved in sea water and applied to the fish. The results showed that effective compounds were included in fractions of molecular weight of several hundreds or lower. (2) Fractions of the extracts obtained by treating with DEAE-Sephadex and CM-Sephadex The ultrafiltrate of the muscle extract was treated with (a) DEAE-Sephadex A-25 anion exchanger, (b) CM-Sephadex C-25 cation exchanger or (c) both of the two ion exchangers in batch procedures. The results obtained showed that (a) reduced the stimulatory effectiveness of the extract. This suggests that some of the effective constituents are adsorbed to the DEAE-Sephadex;(b) had no apparent influence on the effectiveness of the extract; (c) resulted in a sever depression of the effectiveness, suggesting that constituents adsorbing to the cation exchanger also show a feeding- stimulatory effect in cooperation with constituents in the (c)-treated extract.
|
