| Project/Area Number |
03660193
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General fisheries
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
MUROGA Kiyokuni Faculty of Applied Biological Science, Hiroshima University, Professor, 生物生産学部, 教授 (30011993)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIZAWA Toyohiko Faculty of Applied Biological Science, Hiroshima University, Research Associate, 生物生産学部, 助手 (10222184)
NAKAI Toshihiro Faculty of Applied Biological Science, Hiroshima University, Associate Professor, 生物生産学部, 助教授 (60164117)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Seed production / Viral disease / Striped jack / Redspotted grouper / Nodaviridae / ELISA / PCR / 幼生期の病気 / ヘルペスウイルス / ヒラメ |
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| Research Abstract |
In this research project, viral epidermal hyperplasia(VEH) of flounder larvae and viral nervous necrosis(VNN) of striped jack larvae were studied. The results obtained are summarized as follows:
VEH A diagnostic measure using fluorescent antibody technique was developed for this viral infection.
VNN 1) The disease was confirmed to be caused by a virus and this virus (SJNNV) was identified to Nodaviridae based on its morphology and nucleic acid constitution. 2) A diagnostic method using ELISA was applied for this disease, and the detection of viral antigens from eggs indicates a vertical transmission of the disease. 3) The spawners of striped jack proved to be widely infected with the virus(SJNNV), and VNN occurred in larvae obtained from both antibody (anti-SJNNV)-positive and -negative spawners. 4) PCR (polymerase chain reaction) method was applied for the detection of SJNNV by using primers which were composed from analysis of RNA-2 sequence. Selection of virus-free eggs and larvae became possible by using this detection method.
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